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CAPZB Antibody

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Western Blot: CAPZB Antibody [NBP1-85922] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: CAPZB Antibody [NBP1-85922] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & vesicles.
Immunohistochemistry-Paraffin: CAPZB Antibody [NBP1-85922] - Staining of human Testis shows strong cytoplasmic and membranous positivity in cells in seminiferous ducts and Leydig cells.
Western Blot: CAPZB Antibody [NBP1-85922] - Analysis in human cell line A-431.
Immunohistochemistry-Paraffin: CAPZB Antibody [NBP1-85922] - Staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: CAPZB Antibody [NBP1-85922] - Staining of human Placenta shows strong cytoplasmic and membranous positivity in trophoblastic and decidual cells.
Immunohistochemistry-Paraffin: CAPZB Antibody [NBP1-85922] - Staining of human Skeletal muscle shows very weak cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: CAPZB Antibody [NBP1-85922] - Staining of human Tonsil shows strong cytoplasmic and membranous positivity in germinal center and non-germinal center cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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CAPZB Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CALDLMRRLPPQQIEKNLSDLIDLVPSLCEDLLSSVDQPLKIARDKVVGKDYLLCDYNRDGDSYRSPWSNKYDPP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAPZB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
CAPZB Protein (NBP1-85922PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CAPZB Antibody

  • Cap Z
  • CAPB
  • CAPPB
  • capping protein (actin filament) muscle Z-line, beta
  • CapZ beta
  • CAPZ
  • F-actin capping protein beta subunit
  • F-actin-capping protein subunit beta
  • MGC104401
  • MGC129749
  • MGC129750

Background

CAPZB is involved in the regulation of growth of the actin filament by capping the barbed end of growing actin filaments

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CAPZB