![Western Blot: Biotin Antibody [NB120-6643] - Analysis using the Texas Red conjugate of NB120-6643. Detection of Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane.](http://images.novusbio.com/fullsize/Biotin-Antibody-Western-Blot-NB120-6643-img0005.jpg "Western Blot: Biotin Antibody [NB120-6643] - Analysis using the Texas Red conjugate of NB120-6643. Detection of Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, ELISA, IHC, KO |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NB120-6652 | |||
| FITC | NBP1-97372 | |||
| HRP | NB120-6651 | |||
| PE | NBP1-97335 | |||
| Rhodamine | NBP1-97383-0.5mg | |||
| Texas Red | NB110-3647 | |||
| Description | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin. This product is stable for several weeks at 4C as an undiluted liquid. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. |
| Immunogen | Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This product has been tested by ELISA and SDS-PAGE and is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Optimal concentrations in other immunoassays should be determined by the researcher. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NB120-6643 | Applications | Species |
|---|---|---|
| Zhang F, Bazzar W, Alzrigat M, Larsson LG Methods to Study Myc-Regulated Cellular Senescence: An Update Methods in molecular biology (Clifton, N.J.) 2021-05-21 [PMID: 34019294] (IHC-P) | IHC-P |
Secondary Antibodies |
Isotype Controls |
Research Areas for Biotin Antibody (NB120-6643)Find related products by research area.
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![Western Blot: Biotin Antibody [NB120-6643] - Analysis using the Fluorescein conjugate of NB120-6643. Detection of Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane.](http://images.novusbio.com/fullsize/Biotin-Antibody-Western-Blot-NB120-6643-img0004.jpg "Western Blot: Biotin Antibody [NB120-6643] - Analysis using the Fluorescein conjugate of NB120-6643. Detection of Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane.")
![ELISA: Biotin Antibody [NB120-6643] - Analysis using BIOTIN Antibody DyLight 488.](http://images.novusbio.com/fullsize/Biotin-Antibody-ELISA-NB120-6643-img0001.jpg "ELISA: Biotin Antibody [NB120-6643] - Analysis using BIOTIN Antibody DyLight 488.")
![ELISA: Biotin Antibody [NB120-6643] - ELISA results of purified Goat anti-Biotin Antibody Peroxidase Conjugated tested against purified biotin. Each well was coated in duplicate with 1.0 ug of Bovine Albumin Biotin Conjugated. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3 percent fish gelatin as blocking buffer and TMB substrate. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Biotin Antibody-ELISA-NB120-6643-img0007.jpg "ELISA: Biotin Antibody [NB120-6643] - ELISA results of purified Goat anti-Biotin Antibody Peroxidase Conjugated tested against purified biotin. Each well was coated in duplicate with 1.0 ug of Bovine Albumin Biotin Conjugated. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3 percent fish gelatin as blocking buffer and TMB substrate. Image using the HRP form of this antibody.")
