![Western Blot: Bestrophin 1 Antibody (E6-6) - Azide and BSA Free [NBP2-80593] - Western blot analysis of the normal and mutant human Best1 protein in transiently transfected MDCK cells. Best1 proteins are detectable as a 68 kDa band in all transfected cells, but not in non-transfected controls (MDCK lane). Actin bands are shown to indicate equal loading of cell lysates. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/14/7/15121), licensed under a CC-BY license. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Bestrophin-1-Antibody-E6-6-Azide-and-BSA-Free-Western-Blot-NBP2-80593-img0002.jpg "Western Blot: Bestrophin 1 Antibody (E6-6) - Azide and BSA Free [NBP2-80593] - Western blot analysis of the normal and mutant human Best1 protein in transiently transfected MDCK cells. Best1 proteins are detectable as a 68 kDa band in all transfected cells, but not in non-transfected controls (MDCK lane). Actin bands are shown to indicate equal loading of cell lysates. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/14/7/15121), licensed under a CC-BY license. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Po, Ca, Pm, Rt(-)Species Glossary |
| Applications | WB, ICC/IF, IHC, IP, Dual ISH-IHC |
| Clone | E6-6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptide conjugated to KLH corresponding to the C-terminus of human Bestrophin 1 (KDHMDPYWALENRDEAHS) [Uniprot: O76090] |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BEST1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Western blot, this antibody recognizes a band at ~68 kDa representing Bestrophin. Please see protocol for treatment of cell extracts. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Bestrophin 1 Antibody (NBP2-80593)Find related products by research area.
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Vision Infographic: Do you see how I see? Vision involves several parts of the eye processing light which send signals to the brain via the optic nerve to process information. Learn more about the vision process and related ocular proteins in the infographic below. Novus Biological... Read full blog post. |
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Bestrophin 1: Implications in Progressive Vision Loss The human Bestrophin family has four members, Best1, Best2, Best3 and Best4. These transmembrane proteins can function as chloride channels, and can also regulate calcium channels (1). The Bestrophins all have a conserved domain which begins at the N-... Read full blog post. |
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"I can see clearly now": Targeting Bestrophin 1 to treat Bestrophinopathies Encoded by the VMD2 gene on chromosome 11q13 Bestrophin 1 is the prototypic member of the RFP family of proteins which are more commonly called "bestrophins". The protein family was originally identified in Caenorhabditis elegans based on a conserved ... Read full blog post. |
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| Gene Symbol | BEST1 |
![Immunocytochemistry: Bestrophin 1 Antibody (E6-6) - Azide and BSA Free [NBP2-80593] - Bestrophin 1 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Bestrophin 1 (E6-6) Monoclonal Antibody (Catalog # NB300-164) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse](http://images.novusbio.com/fullsize/Bestrophin-1-Antibody-E6-6-Azide-and-BSA-Free-Immunocytochemistry-NBP2-80593-img0005.jpg "Immunocytochemistry: Bestrophin 1 Antibody (E6-6) - Azide and BSA Free [NBP2-80593] - Bestrophin 1 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human Bestrophin 1 (E6-6) Monoclonal Antibody (Catalog # NB300-164) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse")
![Immunohistochemistry: Bestrophin 1 Antibody (E6-6) - Azide and BSA Free [NBP2-80593] - Bestrophin 1 was detected in immersion fixed paraffin sections of human small intestine using t Mouse Anti-Human Bestrophin 1 Monoclonal Antibody (Catalog # NB300-164) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse Ig](http://images.novusbio.com/fullsize/Bestrophin-1-Antibody-E6-6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80593-img0004.jpg "Immunohistochemistry: Bestrophin 1 Antibody (E6-6) - Azide and BSA Free [NBP2-80593] - Bestrophin 1 was detected in immersion fixed paraffin sections of human small intestine using t Mouse Anti-Human Bestrophin 1 Monoclonal Antibody (Catalog # NB300-164) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse Ig")
![Western Blot: Bestrophin 1 Antibody (E6-6) - Azide and BSA Free [NBP2-80593] - Detection of Bestrophin (68 kDa) from human RPE cell lysate. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Bestrophin-1-Antibody-E6-6-Azide-and-BSA-Free-Western-Blot-NBP2-80593-img0001.jpg "Western Blot: Bestrophin 1 Antibody (E6-6) - Azide and BSA Free [NBP2-80593] - Detection of Bestrophin (68 kDa) from human RPE cell lysate. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: Bestrophin 1 Antibody (E6-6) - Azide and BSA Free [NBP2-80593] - X-Z confocal single image scan of transiently transfected cells with different BEST1 cDNA constructs showing mislocalization of mutants Y85H, Q96R, L100R and Y227N. Cells were stained for Best1 (green), beta-catenin (red) and nuclei (blue). Scale bar = 10 um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/14/7/15121), licensed under a CC-BY license. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Bestrophin-1-Antibody-E6-6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80593-img0003.jpg "Immunocytochemistry/Immunofluorescence: Bestrophin 1 Antibody (E6-6) - Azide and BSA Free [NBP2-80593] - X-Z confocal single image scan of transiently transfected cells with different BEST1 cDNA constructs showing mislocalization of mutants Y85H, Q96R, L100R and Y227N. Cells were stained for Best1 (green), beta-catenin (red) and nuclei (blue). Scale bar = 10 um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/14/7/15121), licensed under a CC-BY license. Image from the standard format of this antibody.")
![Dual RNAscope ISH-IHC: Bestrophin 1 Antibody (E6-6) - Azide and BSA Free [NBP2-80593] - Formalin-fixed paraffin-embedded tissue sections of human duodenum were probed for Bestrophin 1 mRNA (ACD RNAScope Probe, catalog # 433181; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human (Novus Biologicals catalog # NB300-164) at 0.3ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). IHC signal is confined to cytoplasm. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Bestrophin-1-Antibody-E6-6-Azide-and-BSA-Free-Dual-RNAscope-ISH-IHC-NBP2-80593-img0006.jpg "Dual RNAscope ISH-IHC: Bestrophin 1 Antibody (E6-6) - Azide and BSA Free [NBP2-80593] - Formalin-fixed paraffin-embedded tissue sections of human duodenum were probed for Bestrophin 1 mRNA (ACD RNAScope Probe, catalog # 433181; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human (Novus Biologicals catalog # NB300-164) at 0.3ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). IHC signal is confined to cytoplasm. Image from the standard format of this antibody.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")