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BAG3 Antibody

Images

 
Western Blot: BAG3 Antibody [NBP2-15543] - BAG3 antibody detects BAG3 protein by Western blot analysis. A. 50 ug mouse heart lysate/extract 7. 5 % SDS-PAGEBAG3 antibody dilution: 1:1000.
Immunohistochemistry-Paraffin: BAG3 Antibody [NBP2-15543] - BAG3 in high-grade serous carcinoma. 1:100 dilution, pH=6 retrieval buffer, Dako FLEX+ protocol. This image was submitted via customer Review.
Western Blot: BAG3 Antibody [NBP2-15543] - BAG3 antibody detects BAG3 protein by Western blot analysis. A. 30 ug K562 whole cell lysate/extract 7. 5 % SDS-PAGEBAG3 antibody dilution: 1:1000.
Immunohistochemistry-Paraffin: BAG3 Antibody [NBP2-15543] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RMSpecies Glossary
Applications WB, IHC, WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

BAG3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human BAG3. The exact sequence is proprietary.
Predicted Species
Rat (95%), Bovine (94%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BAG3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting 1:100 - 1:2000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP2-15543 in the following applications:

Publications
Read Publication using
NBP2-15543 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for BAG3 Antibody

  • BAG family molecular chaperone regulator 3
  • BAG-3
  • BAG-family molecular chaperone regulator-3
  • Bcl-2-associated athanogene 3
  • BCL2-associated athanogene 3
  • BCL2-binding athanogene 3
  • Bcl-2-binding protein Bis
  • BIS
  • CAIR-1
  • Docking protein CAIR-1
  • MGC104307

Background

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for BAG3 Antibody (NBP2-15543)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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IHC-P
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Review for BAG3 Antibody (NBP2-15543) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-15543:
Filter by Applications
IHC-P
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin BAG3 NBP2-15543
Enlarge
3
reviewed by:
Ben Davidson
IHC-P Human 01/30/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman ovarian carcinoma
SpeciesHuman
LotAR100-112619-04

Comments

Comments1:100 dilution, pH=6 retrieval buffer, Dako FLEX+ protocol

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BAG3 Antibody (NBP2-15543) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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5
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3
1
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1
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Ben Davidson
01/30/2017
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol BAG3
Uniprot