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Annexin A2 Antibody

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Knockout Validated: Annexin A2 Antibody [NBP1-31310] - Wild-type (WT) and Annexin II knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin II antibody [N1], N-term. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Genetic Strategies: Knockout Validated: Annexin A2 Antibody [NBP1-31310] - Wild-type (WT) and Annexin II knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ...read more
Western Blot: Annexin A2 Antibody [NBP1-31310] - Analysis. Non-transfected (-) and human Annexin I or Annexin II-transfected (+) whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted ...read more
Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody [NBP1-31310] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Annexin A2 Antibody antibody (Green) at 1:500 ...read more
Immunohistochemistry-Paraffin: Annexin A2 Antibody [NBP1-31310] - Human pancreas tissue, using ANXA2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Western Blot: Annexin A2 Antibody [NBP1-31310] - A. 30 ug A549 whole cell lysate/extract, B. 30 ug HCT116 whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.
Immunohistochemistry-Paraffin: Annexin A2 Antibody [NBP1-31310] - Human pancreas tissue, using ANXA2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Product Details

Summary
Reactivity Hu, Rt, Po, Bv, Ca, Ch, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IP, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

    

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Annexin A2 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Annexin A2. The exact sequence is proprietary.
Localization
Secreted, extracellular space, extracellular matrix, basement membrane, Melanosome
Predicted Species
Rat (100%), Porcine (100%), Bovine (100%), Chicken (93%), Canine (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ANXA2
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500
  • Knockdown Validated
  • Knockout Validated
  • Western Blot 1:500-1:3000
Application Notes
Knockdown validation (PMID: 31585940).
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-31310 in the following applications:

  • ICC/IF
    2 publications
  • IP
    1 publication
  • WB
    3 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31585940).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Annexin A2 Antibody

  • Annexin A2
  • Annexin II
  • annexin-2
  • ANX2
  • ANX2L4
  • ANX2L4LPC2
  • ANXA2
  • CAL1H
  • Calpactin I heavy chain
  • calpactin I heavy polypeptide
  • Calpactin-1 heavy chain
  • chromobindin 8
  • chromobindin-8
  • LIP2
  • LIP2PAP-IV
  • Lipocortin II
  • Lipocortin-2
  • LPC2D
  • LPC2DP36
  • p36
  • PAP-IV
  • Placental anticoagulant protein IV
  • Protein I

Background

The Annexins are a family of structurally similar proteins. Annexins bind to phospholipids and may be involved in regulation of membrane transport, membrane channel activity, and interaction of the cell membrane with the extracellular matrix. Annexin II is a calcium regulated, membrane binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Annexin A2 Antibody (NBP1-31310)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(2)
IP
(1)
WB
(3)
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(2)
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(1)
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Showing Publications 1 - 8 of 8.
Publications using NBP1-31310 Applications Species
Herrera-López EE, Guerrero-Escalera D, Aguirre-Maldonado I et al. Annexins A2 and A5 are potential early biomarkers of hepatocarcinogenesis Scientific reports 2023-04-28 [PMID: 37117324]
Weiss R, Bushi D, Mindel E et al. Autoantibodies to Annexin A2 and cerebral thrombosis: Insights from a mouse model Lupus 2021-02-07 [PMID: 33554716] (WB, Mouse) WB Mouse
Lu H, Xie Y, Tran L et al. Chemotherapy-induced S100A10 recruits KDM6A to facilitate OCT4-mediated breast cancer stemness J Clin Invest. 2020-05-19 [PMID: 32427586] (WB, IP, Human) WB, IP Human
Xiao Y, Zhao H, Tian L et al. S100A10 Is a Critical Mediator of GAS6/AXL-Induced Angiogenesis in Renal Cell Carcinoma Cancer Res 2019-10-06 [PMID: 31585940] (WB, Human) WB Human
Liao L, Zheng B, Yi B et al. Annexin A2-modulated proliferation of pulmonary arterial smooth muscle cells depends on caveolae and caveolin-1 in hepatopulmonary syndrome Exp. Cell Res. 2017-07-17 [PMID: 28729092] (ICC/IF) ICC/IF
Weiss R, Bitton A, Nahary L et al. Cross-reactivity between annexin A2 and Beta-2-glycoprotein I in animal models of antiphospholipid syndrome. Immunol. Res. 2016-07-23 [PMID: 27449504]
Weiss R, Bitton A, Ben Shimon M et al. Annexin A2, autoimmunity, anxiety and depression J. Autoimmun. 2016-06-29 [PMID: 27372915]
Rogers SW, Gahring LC. Upregulation of Nicotinic Acetylcholine Receptor alph4+beta2 through a Ligand-Independent PI3Kbeta Mechanism That Is Enhanced by TNFalpha and the Jak2/p38Mapk Pathways. PLoS One 2015-01-01 [PMID: 26619345] (ICC/IF) ICC/IF

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Annexin A2 Antibody (NBP1-31310). (Showing 1 - 1 of 1 FAQs).

  1. I would like to know how the immunogen was produced and purified.
    • The immunogen is a synthetic peptide, so it was not raised in any expression system. The peptide is conjugated to KLH prior to immunization.

Secondary Antibodies

 

Isotype Controls

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Blogs on Annexin A2.

Check out the latest blog posts on Annexin A2.
Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness
By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax...  Read full blog post.
Integrin Beta 1/CD29 - a cell adhesion and cell signaling protein with diverse functions
Integrins are a large family of trasmembrane proteins involved in cell adhesion and form a link between the intracellular cyskeletal proteins and extracellular matrix proteins. Integrins exist as heterodimers consisting of alpha and beta subunits. ...  Read full blog post.
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Bioinformatics

Gene Symbol ANXA2
Entrez
Uniprot