Aflatoxin B1 ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard curve. Aflatoxin B ELISA Kit is a competitive binding enzyme immunoassay for the quantitative measurement of aflatoxin B. |
| Assay Type |
Competitive ELISA |
| Sample Volume |
25 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
|
Components
|
- 96-wells microtiter plate (#S). Twelve strips of 8 detachable wells, coated with rabbitAnti-Aflatoxin antibody
- Aflatoxin-Alkaline Phosphatase conjugate (AFX-ALP). (#3)
- Calibrator containing 0, 0.5, 2.0 and 8.0 ng/mL of Aflatoxin B1
- p-Nitrophenyl Phosphate (pNPP) substrate. Ready to use. (#5)
- Stop Solution, 3 N NaOH. (#7)
- Wash Buffer (10xPBS-Tween). Dilute 10 fold with distilled or deionized water to 150mL prior to use. (#6)
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Aflatoxin B1 ELISA Kit (Colorimetric)
Background
Aflatoxin B1 is a naturally occurring mycotoxin produced by two types of mold: Aspergillus flavus and Aspergillus parasiticus. These molds commonly affect grains which are grown under stressful conditions such as drought and also grows on food that is stored in hot and humid conditions.
There are 13 known Aflatoxins produced, with aflatoxin B1 considered as the most toxic. In fact, Aflatoxin B1 is the most potent hepatocellular carcinoma (HCC) causing chemical currently known. Specifically, Aflatoxin B1 is thought to case mutations in the p53 gene which interfere with the gene's critical tumor suppressing functions.
Aflatoxin B1 are useful tools for studying hepatocellular carcinoma formation with wide ranging implications into public health and food saftey.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Aflatoxin B1 ELISA Kit (KA1412). (Showing 1 - 1 of 1 FAQ).
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My question was how sensitive is your most sensitive aflatoxin antibody? Say in a standard ELISA plate format. I could not find any info of that kind on your website.
- We have one kit available, cat# KA1412, that can detect Aflatoxin levels of less than 1 ng/mL. The only other sensitivity information we have is that NB120-1017 can detect Aflatoxin when used at a concentration of 5ug/mL and NB100-2908 has a recommended dilution of 1:2000-1:10000 for use in ELISA.
![ELISA: Aflatoxin B1 ELISA Kit (Colorimetric) [KA1412] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.](http://images.novusbio.com/fullsize/Aflatoxin-B1-ELISA-Kit-Colorimetric-ELISA-KA1412-img0001.jpg "ELISA: Aflatoxin B1 ELISA Kit (Colorimetric) [KA1412] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.")
![Dot Blot: Aflatoxin B1 Antibody (6A10) [NB600-443] - HCT-8 cells stably-transfected with empty vector or shCYP3A5 were treated with DMSO or 10 mM AFB1 for 72 h. AFB1 DNA adduct (6A10) were detected using immunodot-blot assay. DNA adduct measured by multi gauge software (bottom panel), respectively. Image collected and cropped by CiteAb from the following publication (oncotarget.com/article/8914/text/), licensed under a CC-BY license.](https://images.novusbio.com/images/Aflatoxin-B1-Antibody-6A10-Dot-Blot-NB600-443-img0004.jpg "Dot Blot: Aflatoxin B1 Antibody (6A10) [NB600-443] - HCT-8 cells stably-transfected with empty vector or shCYP3A5 were treated with DMSO or 10 mM AFB1 for 72 h. AFB1 DNA adduct (6A10) were detected using immunodot-blot assay. DNA adduct measured by multi gauge software (bottom panel), respectively. Image collected and cropped by CiteAb from the following publication (oncotarget.com/article/8914/text/), licensed under a CC-BY license.")