58K Golgi Protein Antibody (58K-9)

The 58K Golgi protein is one of the most commonly used Golgi markers and has been proposed to implicate in linking Golgi to microtubules and it peripherally associates with cytosolic surface of Golgi membranes. This protein shows extensive overall sequence identity with porcine FTCD and has been suggested to represent rat FTCD (formiminotransferase-cyclodeaminase), a metabolic enzyme involved in the conversion of histidine to glutamic acid. FTCD's formiminotransferase activity facilitate transfer of a a formimino group from N-formimino-L-glutamic acid to tetrahydrofolate to generate glutamic acid and 5-formiminotetrahydrofolate, and the cyclodeaminase activity then converts the 5-formiminotetrahydrofolate to 5,10-methenyl tetrahydrofolate and ammonia. FTCD appears to be a dynamic component of Golgi and cycles between Golgi and earlier compartments of the secretory pathway. It also binds and promotes bundling of vimentin filaments originating from the Golgi, and mutations in this gene encoding for 58K Golgi Protein i.e. FTCD are associated with glutamate formiminotransferase deficiency (FIGLU-URIA).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 3 applications: ICC/IF, IHC-Fr, WB.

Showing Publications 1 - 10 of 15. Show All 15 Publications. Collapse Publications.

Publications using NB600-412	Applications	Species
Tao Yu, Dan Hou, Jiaqi Zhao, Xuan Lu, Wendy K. Greentree, Qian Zhao, Min Yang, Don-Gerard Conde, Maurine E. Linder, Hening Lin NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation Cell reports 2024-05-22 [PMID: 38583156]
Andreeva L, David L, Rawson S et al. NLRP3 cages revealed by full-length mouse NLRP3 structure control pathway activation Cell 2021-12-22 [PMID: 34861190]
Matteo Spinelli, Salvatore Fusco, Marco Mainardi, Federico Scala, Francesca Natale, Rosita Lapenta, Andrea Mattera, Marco Rinaudo, Domenica Donatella Li Puma, Cristian Ripoli, Alfonso Grassi, Marcello D’Ascenzo, Claudio Grassi Brain insulin resistance impairs hippocampal synaptic plasticity and memory by increasing GluA1 palmitoylation through FoxO3a Nature Communications 2017-12-08 [PMID: 29222408]
Martin PT, Golden B, Okerblom J et al. A Comparative Study of N-glycolylneuraminic Acid (Neu5Gc) and Cytotoxic T Cell (CT) Carbohydrate Expression in Normal and Dystrophin-Deficient Dog and Human Skeletal Muscle. PLoS ONE 2014-02-07 [PMID: 24505439] (IHC-Fr, Human)	IHC-Fr	Human
Murphy AJ, Bijl N, Yvan-Charvet L et al. Cholesterol efflux in megakaryocyte progenitors suppresses platelet production and thrombocytosis. Nat Med 2013-05-01 [PMID: 23584088] (ICC/IF, Mouse)	ICC/IF	Mouse
Nakonechnaya AO, Jefferson HS, Chen X, Shewchuk BM. Differential effects of exogenous and autocrine growth hormone on LNCaP prostate cancer cell proliferation and survival J Cell Biochem 2012-12-13 [PMID: 23238889] (ICC/IF, Human)	ICC/IF	Human
Azad AK, Torrelles JB, Schlesinger LS. Mutation in the DC-SIGN cytoplasmic triacidic cluster motif markedly attenuates receptor activity for phagocytosis and endocytosis of mannose-containing ligands by human myeloid cells. J Leukoc Biol;84(6):1594-603. 2008-12-01 [PMID: 18772280]
Young KG, Pinheiro B, Kothary R. A Bpag1 isoform involved in cytoskeletal organization surrounding the nucleus. Exp Cell Res;312(2):121-34. 2006-01-15 [PMID: 16289082] (ICC/IF, Rat, Mouse)	ICC/IF	Rat, Mouse
Gao YS, Alvarez C, Nelson DS, Sztul E. Molecular cloning, characterization, and dynamics of rat formiminotransferase cyclodeaminase, a Golgi-associated 58-kDa protein. J Biol Chem;273(50):33825-34. 1998-12-11 [PMID: 9837973] (ICC/IF, Rat)	ICC/IF	Rat
Bashour AM, Bloom GS. 58K, a microtubule-binding Golgi protein, is a formiminotransferase cyclodeaminase. J Biol Chem;273(31):19612-7. 1998-07-31 [PMID: 9677387] (WB, Rat)	WB	Rat
Hennig D, Scales SJ, Moreau A, Murley LL, De Mey J, Kreis TE. A formiminotransferase cyclodeaminase isoform is localized to the Golgi complex and can mediate interaction of trans-Golgi network-derived vesicles with microtubules. J Biol Chem;273(31):19602-11. 1998-07-31 [PMID: 9677386] (WB, ICC/IF, Human, Chicken)	WB, ICC/IF	Human, Chicken
Lippincott-Schwartz J, Cole NB, Marotta A, Conrad PA, Bloom GS. Kinesin is the motor for microtubule-mediated Golgi-to-ER membrane traffic. J Cell Biol;128(3):293-306. 1995-02-01 [PMID: 7844144] (ICC/IF, Rat, Human)	ICC/IF	Rat, Human
Bloom GS, Brashear TA. A novel 58-kDa protein associates with the Golgi apparatus and microtubules. J Biol Chem 1989-09-25 [PMID: 2777777] (WB, ICC/IF, Rat)	WB, ICC/IF	Rat
Zhou A, Dong X, Tang B TNK2/ACK1 Strengthen Influenza A Virus Infection by Blocking Viral Matrix 2 Protein(M2) into Lysosome to Degradation Research Square 2021-01-29 (ICC/IF)	ICC/IF
Bharat V, Hsieh CH, Wang X Mitochondrial Defects in Fibroblasts of Pathogenic MAPT Patients Frontiers in cell and developmental biology 2021-11-03 [PMID: 34805172] (WB, Human)	WB	Human
Show All 15 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	2 reviewed by: Verified Customer	ICC	Mouse	03/22/2023	View Summary Application Immunocytochemistry Sample Tested Mouse Brain Endothelial Cells Species Mouse Lot A-8 Comments Comments ***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
	4 reviewed by: Ali Almousawi	ICC	Mouse	03/13/2023	View Summary Application Immunocytochemistry Sample Tested Cultured Brain Endothelial cells,Cultured Mouse brain endothelial cells,Mouse Brain Endothelial cells (mBECs) Species Mouse Lot A-8
Enlarge	4 reviewed by: Verified Customer	ICC	Mouse	11/19/2018	View Summary Application Immunocytochemistry Sample Tested mouse vascular smooth muscle cell Species Mouse Lot A2

Array NB600-412

• 58K Golgi Protein Antibodies
• 58K Golgi Protein Lysates
• 58K Golgi Protein Proteins