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4-1BB Ligand/TNFSF9 Antibody (282220) [Alexa Fluor® 647]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
282220
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 647
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

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4-1BB Ligand/TNFSF9 Antibody (282220) [Alexa Fluor® 647] Summary

Immunogen
E. coli-derived recombinant human 4‑1BB Ligand/TNFSF9
Arg71-Glu254
Accession # P41273.1
Specificity
Detects human 4‑1BB Ligand/TNFSF9 Ligand in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) 4-1BB Ligand, recobinant human (rh) APRIL, rhBAFF, rmEDA-A2, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhLymphotoxin alpha 1/ beta 2, rhLymphotoxin alpha 2/ beta 1, rhOX40 Ligand, rhTNF-alpha , rhTRAIL, rhTRANCE, or rhTWEAK is observed.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TNFSF9
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 4-1BB Ligand/TNFSF9 Antibody (282220) [Alexa Fluor® 647]

  • 41BB Ligand
  • 4-1BB Ligand
  • 4-1BBL
  • 4-1BB-L
  • CD137L
  • homolog of mouse 4-1BB-L
  • receptor 4-1BB ligand
  • TNFSF9
  • tumor necrosis factor (ligand) superfamily, member 9
  • tumor necrosis factor ligand superfamily member 9

Background

4-1BB Ligand (4-1BBL; also CD137L) is a 32 kDa type II transmembrane protein that belongs to the TNF superfamily (TNFSF) molecules (1‑4). The human 4-1BBL cDNA encodes a 254 amino acid (aa) protein that contains a 25 aa N-terminal cytoplasmic domain, a 23 aa transmembrane segment, and a 206 aa C-terminal extracellular region (5). The extracellular domain (ECD) of 4-1BBL has a jelly-roll, beta -sandwich tertiary structure that is similar to other TNFSF members. There is only one cysteine in the human ECD, and no potential N-linked glycosylation sites. The potential exists, however, for O-linked glycosylation. The human 4-1BBL ECD shares 32% and 35% aa identity with mouse and rat ECD, respectively. In the cytoplasmic domain, human 4-1BBL is 55 aa shorter than the equivalent region in rodents. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated T cells, neurons and astrocytes (2, 3, 6). A bioactive 26 kDa soluble form of 4-1BBL, presumably generated by MMP cleavage, occurs in humans (4). Human 4-1BBL signals through both CD137/4-1BB and itself. Its cytoplasmic tail participates in reverse signaling that induces apoptosis in T cells and cytokine secretion (IL-6; TNF-alpha ) by monocytes (7, 8). 4-1BBL binding to CD137/4-1BB produces a number of effects. It seems to play a key role in the T cell recall response. It maintains T cell numbers at the end of a primary response, and induces CD4+ and CD8+ T cells to proliferate and secrete cytokines such as IL-2 and IFN-gamma in CD4+ cells, and IFN-gamma in CD8+ cells (9, 10).

  1. Kwon, B. et al. (2003) Exp. Mol. Med. 35:8.
  2. Salih, H.R. et al. (2002) Int. J. Clin. Pharmacol. Ther. 40:348.
  3. Vinay, D.S. and B.S. Kwon (1998) Semin. Immunol. 1:481.
  4. Salih, H.R. et al. (2001) J. Immunol. 167:4059.
  5. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
  6. Reali, C. et al. (2003) J. Neurosci. Res. 74:67.
  7. Michel, J. et al. (1999) Immunology 98:42.
  8. Langstein, J. et al. (1998) J. Immunol. 160:2488.
  9. Wen, T. et al. (2002) J. Immunol. 168:4897.
  10. Dawicki, W. and T.H. Watts (2004) Eur. J. Immunol. 34:743.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TNFSF9
Uniprot