![Western Blot: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - Affinity Purified PPAR delta/NR1C2/NR1C2 antibody is shown to detect a predominant band at ~ 40kDa corresponding to PPAR delta/NR1C2 present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30ug of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4 C followed reaction with a 1:10,000 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green). IRDye(R) 800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody---N-terminal-Western-Blot-NB600-637-img0007.jpg "Western Blot: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - Affinity Purified PPAR delta/NR1C2/NR1C2 antibody is shown to detect a predominant band at ~ 40kDa corresponding to PPAR delta/NR1C2 present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30ug of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4 C followed reaction with a 1:10,000 dilution of IRDye(R) 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green). IRDye(R) 800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This affinity purified antibody is directed against mouse PPAR delta/NR1C2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | PPAR delta/NR1C2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of mouse PPAR delta/NR1C2. (Uniprot: P35396) |
| Localization | Nuclear |
| Specificity | No reactivity is expected against other subtypes of PPAR. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PPARD |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This affinity purified antibody has been tested for use in ELISA, IHC, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a single band approximately 43 kDa in size corresponding to PPAR delta by western blot in the appropriate tissue or cell lysate. IHC-P use reported by customer in Gerbil stomach tissue. |
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| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
IHC-P | Other | 01/28/2010 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for PPAR delta/NR1C2 Antibody (NB600-637)Find related products by research area.
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![Immunohistochemistry: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - Immunohistochemistry of PPAR delta/NR1C2/NR1C2 antibody. Antigen Retrieval: HIER pH 6.2. [Right] -Neg Ctrl: Normal rabbit IgG on heart striated muscle, pH6.2 (40X). [Left Top - low mag, Left Bottom - 20X] -Staining: Diffuse cytoplasmatic positivity for PPAR delta/NR1C2 in human myocardiocitys at 1:500.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody---N-terminal-Immunohistochemistry-NB600-637-img0008.jpg "Immunohistochemistry: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - Immunohistochemistry of PPAR delta/NR1C2/NR1C2 antibody. Antigen Retrieval: HIER pH 6.2. [Right] -Neg Ctrl: Normal rabbit IgG on heart striated muscle, pH6.2 (40X). [Left Top - low mag, Left Bottom - 20X] -Staining: Diffuse cytoplasmatic positivity for PPAR delta/NR1C2 in human myocardiocitys at 1:500.")
![ELISA: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - ELISA results of purified PPAR delta/NR1C2 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/PPAR-delta-NR1C2-Antibody---N-terminal-ELISA-NB600-637-img0009.jpg "ELISA: PPAR delta/NR1C2 Antibody - N-terminal [NB600-637] - ELISA results of purified PPAR delta/NR1C2 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.")
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