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PLD1 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: PLD1 Antibody [NBP2-34078] - Staining in human gallbladder and skeletal muscle tissues using anti-PLD1 antibody. Corresponding PLD1 RNA-seq data are presented ...read more
Immunocytochemistry/ Immunofluorescence: PLD1 Antibody [NBP2-34078] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PLD1 Antibody [NBP2-34078] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: PLD1 Antibody [NBP2-34078] - Staining of human gallbladder shows strong positivity in glandular cells.
Immunohistochemistry-Paraffin: PLD1 Antibody [NBP2-34078] -Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PLD1 Antibody [NBP2-34078] -Staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: PLD1 Antibody [NBP2-34078] -Staining of human kidney shows moderate cytoplasmic positivity in cells in glomeruli.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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PLD1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: NEPRVNTSALQKIAADMSNIIENLDTRELHFEGEEVDYDVSPSDPKIQEVYIPFSAIYNTQGFKEPNIQTYLSGCPIKAQVLEVERFTSTTRVPSINLYTIELTHGEFKWQVKRKFKHFQEFHR
Predicted Species
Mouse (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PLD1 Protein (NBP2-34078PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PLD1 Antibody

  • Choline phosphatase 1
  • EC 3.1.4.4
  • hPLD1
  • Phosphatidylcholine-hydrolyzing phospholipase D1
  • phospholipase D1
  • phospholipase D1, phophatidylcholine-specific
  • phospholipase D1, phosphatidylcholine-specific
  • PLD 1
  • PLD1

Background

Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC-specific PLD activity has been implicated in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis (Hammond et al., 1995 [PubMed 8530346]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PLD1
Uniprot