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Nucleostemin Antibody [Unconjugated]

 (9 Publications)
 
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Western blot shows lysates of PC-3 human prostate cancer cell line and SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse/Rat Nucleostemin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1638) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Nucleostemin at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of PC-3 human prostate cancer cell line and SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse/Rat Nucleostemin Antigen ...read more
Nucleostemin was detected in immersion fixed U2OS human osteosarcoma cell line using 10 µg/mL Goat Anti-Human/Mouse/Rat Nucleostemin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1638) for 3 hours at room ...read more
Simple Western lane view shows lysates of PC‑3 human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Nucleostemin at approximately 83 kDa (as indicated) using 5 µg/mL of Goat ...read more
Embryonic stem cell markers. Immunohistochemistry: A. NANOG; B. OCT4. The majority of nuclei in the NANOG IHC are brown indicating the presence of NANOG. Some, but not all, nuclei are positive for OCT4. Negative ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF1638-SP
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Nucleostemin Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant rat Nucleostemin
Lys2-Ile538
Accession # Q811S9
Specificity
Detects human, mouse, rat Nucleostemin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant rat Nucleostemin C-terminal peptide (aa 281-538) is observed. Rat Nucleostemin specific IgG was purified by first passing the sera over a rat Nucleostemin aa 2-538 column and then passing the bound fraction over a rat Nucleostemin aa 281-538 column to removed rat Nucleostemin C-terminal specific IgG. 
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GNL3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.25 ug/mL
Publications
Read Publications using
AF1638 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nucleostemin Antibody [Unconjugated]

  • C77032
  • E2IG3NNP47
  • estradiol-induced nucleotide binding protein
  • GNL3
  • guanine nucleotide binding protein-like 3 (nucleolar)
  • guanine nucleotide-binding protein-like 3
  • MGC800
  • Novel nucleolar protein 47
  • NSE2-induced gene 3 protein
  • Nucleolar GTP-binding protein 3
  • Nucleostemin

Background

Nucleostemin is a protein found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell-cycle progression in stem cells and cancer cells (1-3).
  1. Tsai, R.Y. and R.D. McKay (2002) Genes Dev. 16:2991.
  2. Baddoo, M. et al. (2003) J. Cell Biochem. 89:1235.
  3. Normile, D. (2002) Science 298:1869.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nucleostemin Antibody (AF1638)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC, IHC-Fr, IHC-P, Western Blot.


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ICC
(4)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(3)
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Human
(5)
Mouse
(1)
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Showing Publications 1 - 9 of 9.
Publications using AF1638 Applications Species
J Gil-Ranedo, C Gallego-Ga, JM Almendral Viral targeting of glioblastoma stem cells with patient-specific genetic and post-translational p53 deregulations Cell Reports, 2021-09-07;36(10):109673. 2021-09-07 [PMID: 34496248] (ICC, Human) ICC Human
Mohamed Kodiha, Hicham Mahboubi, Dusica Maysinger, Ursula Stochaj Gold Nanoparticles Impinge on Nucleoli and the Stress Response in MCF7 Breast Cancer Cells Nanobiomedicine (Rij) 1/1/2016 [PMID: 29942378]
Yoshida , R, Nakayama , H, Nagata , M, Hirosue , A, Tanaka , T, Kawahara , K, Nakagawa , Y, Matsuoka , Y, Sakata , J, Arita , H, Hiraki , A, Shinohara , M, Ito , T Overexpression of nucleostemin contributes to an advanced malignant phenotype and a poor prognosis in oral squamous cell carcinoma. Br J Cancer, 2014-10-14;111(12):2308-15. 2014-10-14 [PMID: 25314067] (IHC-P, Western Blot, Human) IHC-P, Western Blot Human
Sauder C, Koziel J, Choi M, Fox M, Grimes B, Badve S, Blosser R, Radovich M, Lam C, Vaughan M, Herbert B, Clare S Phenotypic plasticity in normal breast derived epithelial cells. BMC Cell Biol, 2014-06-10;15(0):20. 2014-06-10 [PMID: 24915897] (ICC, Human) ICC Human
Haitong Su, Mohamed Kodiha, Sunghoon Lee, Ursula Stochaj Identification of Novel Markers That Demarcate the Nucleolus during Severe Stress and Chemotherapeutic Treatment PLoS ONE 11/6/2013 [PMID: 24223222]
Yamashita M, Nitta E, Nagamatsu G, Ikushima Y, Hosokawa K, Arai F, Suda T Nucleostemin is indispensable for the maintenance and genetic stability of hematopoietic stem cells. Biochem Biophys Res Commun, 2013-10-16;441(1):196-201. 2013-10-16 [PMID: 24140061] (IHC-Fr, Mouse) IHC-Fr Mouse
E. De Nevi, P. Marco-Salazar, D. Fondevila,1, E. Blasco, L. Pérez, M. Pumarola Immunohistochemical study of doublecortin and nucleostemin in canine brain European Journal of Histochemistry 3/20/2013 [PMID: 23549468]
Huang M, Whang P, Chodaparambil JV Reactive oxygen species regulate nucleostemin oligomerization and protein degradation. J. Biol. Chem., 2011-01-17;286(13):11035-46. 2011-01-17 [PMID: 21242306] (ICC, Western Blot, Human) ICC, Western Blot Human
Huang M, Itahana K, Zhang Y, Mitchell BS Depletion of guanine nucleotides leads to the Mdm2-dependent proteasomal degradation of nucleostemin. Cancer Res., 2009-03-24;69(7):3004-12. 2009-03-24 [PMID: 19318567] (ICC, Western Blot, Human) ICC, Western Blot Human

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Bioinformatics

Gene Symbol GNL3
Uniprot