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NDUFB6 Antibody

Images

 
Western Blot: NDUFB6 Antibody [NBP1-92172] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). ...read more
Immunocytochemistry/ Immunofluorescence: NDUFB6 Antibody [NBP1-92172] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.
Immunohistochemistry-Paraffin: NDUFB6 Antibody [NBP1-92172] - Staining of human prostate shows strong cytoplasmic positivity with granular pattern in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NDUFB6 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LRELRRRWLKDQELSPREPVLPPQKMGPMEKFWNK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NDUFB6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NDUFB6 Protein (NBP1-92172PEP)
Publications
Read Publications using
NBP1-92172 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (83%). Human reactivity reported in scientific literature (PMID: 25605331).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NDUFB6 Antibody

  • B17
  • CI
  • CI-B17
  • complex I, mitochondrial respiratory chain, B17 subunit
  • Complex I-B17
  • MGC13675
  • NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6 (17kD, B17)
  • NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa
  • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
  • NADH-ubiquinone oxidoreductase B17 subunit
  • NADH-ubiquinone oxidoreductase beta subunit, 6

Background

NDUFB6 is encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NDUFB6 Antibody (NBP1-92172)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IF/IHC, WB.


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IF/IHC
(1)
WB
(2)
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Human
(2)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NDUFB6