Novus Biologicals products are now on bio-techne.com

Myoglobin Antibody (2269B) [Unconjugated]

Images

 
Western blot shows lysates of human heart tissue, mouse heart tissue, rat heart tissue, and human liver tissue. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Myoglobin Monoclonal Antibody (Catalog # ...read more
Myoglobin was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human Myoglobin Monoclonal Antibody (Catalog # MAB97203) at 3 µg/mL for 1 hour at room temperature followed ...read more
Simple Western lane view shows lysates of human heart tissue, mouse heart tissue, rat heart tissue, and human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Myoglobin at approximately 21-24 ...read more
Recombinant Human Myoglobin protein was serially diluted 2-fold and captured by Rabbit Anti-Human Myoglobin Monoclonal Antibody (Catalog # MAB97204) coated on a Clear Polystyrene Microplate (Catalog # DY990). ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ELISA, IHC
Clone
2269B
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Myoglobin Antibody (2269B) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Purifed human cardiac Myoglobin antigen from human heart
Accession # P02144
Specificity
Detects human Myoglobin in direct ELISAs. Detects human, mouse, and rat Myoglabin in Western blots.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry 3-25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myoglobin Antibody (2269B) [Unconjugated]

  • MB
  • MGC13548
  • Myoglobin
  • PVALB

Background

Myoglobin is a 17KDa cytoplasmic oxygen-binding protein encoded by the MB gene and expressed in myocytes of the heart and skeletal muscle. Its name derives from its structural and functional similarity to hemoglobin, the oxygen binding protein found in red blood cells. Functions of myoglobin include oxygen storage and transport, as well as scavenging of NO and reactive oxygen species. Myoglobin also serves as a sensitive marker for muscle injury resulting from cardiac infarction. Myoglobin was the first protein to have its three-dimensional structure determined by X-ray crystallography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-85630
Species: Hu
Applications: IHC, IHC-P, WB
8184-CK
Species: Hu
Applications: EnzAct
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
NB100-56503
Species: Dr, Hu, Mu, Rb, Rt
Applications: Flow-CS, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, Simple Western, WB
AF3844
Species: Hu, Mu
Applications: IHC
NBP2-61118
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
NBP2-35336
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NB300-223
Species: Bv, Ca, Ch, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
664-LI
Species: Hu
Applications: BA
NBP2-46395
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
AF1107
Species: Mu
Applications: IHC, WB
H00003347-M01
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
NB600-922
Species: Ch
Applications: ELISA, IHC, IP, Single-Cell Western, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
DY1707
Species: Hu
Applications: ELISA

Publications for Myoglobin Antibody (MAB97203) (0)

There are no publications for Myoglobin Antibody (MAB97203).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Myoglobin Antibody (MAB97203) (0)

There are no reviews for Myoglobin Antibody (MAB97203). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Myoglobin Antibody (MAB97203) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Myoglobin Antibody (2269B) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Uniprot