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MCAF1 Antibody

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Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Analysis in human testis and skeletal muscle tissues. Corresponding MCAF1 RNA-seq data are presented for the same tissues.
Orthogonal Strategies: Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Analysis in human testis and skeletal muscle tissues. Corresponding MCAF1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: MCAF1 Antibody [NBP1-84610] - Staining of human cell line A-431 shows positivity in nuclei but not nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Staining of human lymph node shows moderate nuclear positivity in germinal center cells.
Immunohistochemistry-Paraffin: MCAF1 Antibody [NBP1-84610] - Staining of human skeletal muscle shows low nuclear positivity in myocytes as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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MCAF1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SMEIDQGEKNEDETSADLVETINENVIEDNKSENILENTDSMETDEIIPILEKLAPSEDELTCFSKTSLLPIDETNPDLEEKMESSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATF7IP
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000-1:2500
  • Knockdown Validated
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MCAF1 Protein (NBP1-84610PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-84610 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MCAF1 Antibody

  • activating transcription factor 7 interacting protein
  • activating transcription factor 7-interacting protein 1
  • ATF7-interacting protein
  • ATFa-associated modulator
  • ATF-interacting protein
  • ATF-IP
  • FLJ10139
  • FLJ10688
  • hAM
  • MBD1-containing chromatin associated factor
  • MCAF1AM
  • MCAFMBD1-containing chromatin-associated factor 1
  • MMCP 6
  • MMCP6
  • MMCP-6
  • p621

Background

MBD1-containing chromatin-associated factor (MCAF) is a 1270-residue mediator in the DNA methylation cycle of a variety of genomic functions. This nuclear protein will bind to the C-terminal transcriptional repression domain of MBD1 to form a blocking complex. This molecule will then prevent transcription from methylated promoters in a histone deacetylation-independent action (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MCAF1 Antibody (NBP1-84610) (0)

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Review for MCAF1 Antibody (NBP1-84610) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-84610:
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(1)
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Images Ratings Applications Species Date Details
Western Blot MCAF1 NBP1-84610
Enlarge 		4
reviewed by:
Wei-Ting Lu
 WB Human 01/18/2019
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Summary

ApplicationWestern Blot
Sample TestedH1944 cell line
SpeciesHuman
Lotr09412

Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Recent Reviews

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Western Blot MCAF1 NBP1-84610
Enlarge
 Wei-Ting Lu
01/18/2019
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol ATF7IP