![Western Blot: Human Lymph Node Whole Tissue Lysate (Adult Whole Normal) [NB820-59242] - Protein lysate can be validated by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.](http://images.novusbio.com/fullsize/Human-Lymph-Node-Whole-Tissue-Lysate-Adult-Whole-Normal-Western-Blot-NB820-59242-img0001.jpg "Western Blot: Human Lymph Node Whole Tissue Lysate (Adult Whole Normal) [NB820-59242] - Protein lysate can be validated by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Concentration | 5.0 mg/ml |
| Description | We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use. |
| Specificity | Total Protein - Human Adult Normal Tissue: Lymph Node |
| Dilutions |
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| Application Notes | Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors |
| Concentration | 5.0 mg/ml |
| Type | Tissue |
| Life Stage | Adult |
| Tissue | Blood Lymph |
| Protein State | Native |
| Tissue Condition | Normal |
| Subcellular Fraction | Whole |
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