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HAI-1/HGFA Inhibitor 1 Antibody [Unconjugated]

 (8 Publications)
 
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Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HAI-1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) followed by ...read more
HAI-1 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Goat Anti-Human HAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) at 5 µg/mL overnight at 4 °C. ...read more
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for HAI‑1 at approximately 88 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
Carrier Free
AF1048-SP
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HAI-1/HGFA Inhibitor 1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human HAI-1
Pro37-Glu449
Accession # NP_003701
Specificity
Detects human HAI-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse
HAI‑1 is observed and less than 1% cross-reactivity with recombinant human HAI-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SPINT1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF1048 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HAI-1/HGFA Inhibitor 1 Antibody [Unconjugated]

  • HAI
  • HAI1
  • HAI-1
  • hepatocyte growth factor activator inhibitor 1
  • Hepatocyte growth factor activator inhibitor type 1
  • kunitz-type protease inhibitor 1
  • MANSC2
  • serine peptidase inhibitor, Kunitz type 1
  • serine protease inhibitor, Kunitz type 1
  • SPINT1

Background

HAI-1 is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matriptase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate 13-acetate or IL-1 beta . The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).

  1. Denda, K. et al. (2002) J. Biol. Chem. 277:14053. 
  2. Shimomura, T. et al. (1997) J. Biol. Chem. 272:6370.
  3. Kataoka, H. et al. (2000) J. Biol. Chem. 275:40453.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HAI-1/HGFA Inhibitor 1 Antibody (AF1048)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-P, Western Blot.


Filter By Application
IHC-P
(2)
Western Blot
(6)
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Human
(7)
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Showing Publications 1 - 8 of 8.
Publications using AF1048 Applications Species
Hatzold, J;Nett, V;Brantsch, S;Zhang, JL;Armistead, J;Wessendorf, H;Stephens, R;Humbert, PO;Iden, S;Hammerschmidt, M; Matriptase-dependent epidermal pre-neoplasm in zebrafish embryos caused by a combination of hypotonic stress and epithelial polarity defects PLoS genetics 2023-08-11 [PMID: 37566613] (Western Blot, Human) Western Blot Human
CJ Wu, M Lu, X Feng, G Nakato, MC Udey Matriptase Cleaves EpCAM and TROP2 in Keratinocytes, Destabilizing Both Proteins and Associated Claudins Cells, 2020-04-21;9(4):. 2020-04-21 [PMID: 32326212] (Western Blot, Human) Western Blot Human
X Du, L Wu, MS Ur Rahman, X Teng, L Teng, J Ye, J Cao Promoter Hypomethylation Is Responsible for Upregulated Expression of HAI-1 in Hepatocellular Carcinoma Dis. Markers, 2019-08-28;2019(0):9175215. 2019-08-28 [PMID: 31558918] (IHC-P, Western Blot, Human) IHC-P, Western Blot Human
T Ishikawa, Y Kimura, H Hirano, S Higashi Matrix metalloproteinase-7 induces homotypic tumor cell aggregation via proteolytic cleavage of the membrane-bound Kunitz-type inhibitor HAI-1 J. Biol. Chem., 2017-10-18;0(0):. 2017-10-18 [PMID: 29046355] (Western Blot, Human) Western Blot Human
AS Murray, FA Varela, TE Hyland, AJ Schoenbeck, JM White, LM Tanabe, SV Todi, K List Phosphorylation of the type II transmembrane serine protease, TMPRSS13 in Hepatocyte Growth Factor Activator Inhibitor-1 and 2-mediated cell surface localization J. Biol. Chem., 2017-07-14;0(0):. 2017-07-14 [PMID: 28710277] (Western Blot, Human) Western Blot Human
Erik W. Martin, Marguerite S. Buzza, Kathryn H. Driesbaugh, Shihui Liu, Yolanda M. Fortenberry, Stephen H. Leppla et al. Targeting the membrane-anchored serine protease testisin with a novel engineered anthrax toxin prodrug to kill tumor cells and reduce tumor burden Oncotarget 2015-10-20 [PMID: 26392335]
Buzza M, Martin E, Driesbaugh K, Desilets A, Leduc R, Antalis T Prostasin is required for matriptase activation in intestinal epithelial cells to regulate closure of the paracellular pathway. J Biol Chem, 2013-02-26;288(15):10328-37. 2013-02-26 [PMID: 23443662] (Western Blot, Human) Western Blot Human
Szabo R, Molinolo A, List K, Bugge TH Matriptase inhibition by hepatocyte growth factor activator inhibitor-1 is essential for placental development. Oncogene, 2006-09-18;26(11):1546-56. 2006-09-18 [PMID: 16983341] (IHC-P, Human) IHC-P Human

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Bioinformatics

Gene Symbol SPINT1
Uniprot