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![Western Blot: PrPSc Antibody (3F4) [NBP2-81078] - Human brain cerebral cortex (A), cerebellum (B), and hippocampus (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of PrPSc antibody (3F4) [NBP2-81078]. Cerebral cortex and hippocampus samples were probed using 0.001 ug/ml of NBP2-81078, and cerebellum samples with 0.003 ug/ml of NBP2-81078, before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for unmodified major Prion protein is 27.7 kDa though this protein has several glycosylated forms, may be lipidated, sumoylated and post-translationally cleaved to produce the matrue form of the protein [Uniprot]. NBP2-81078 successfully detected major Prion protein in human brain cerebral cortex, cerebellum, and hippocampus lysates.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-3F4-Western-Blot-NBP2-81078-img0002.jpg "Western Blot: PrPSc Antibody (3F4) [NBP2-81078] - Human brain cerebral cortex (A), cerebellum (B), and hippocampus (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of PrPSc antibody (3F4) [NBP2-81078]. Cerebral cortex and hippocampus samples were probed using 0.001 ug/ml of NBP2-81078, and cerebellum samples with 0.003 ug/ml of NBP2-81078, before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for unmodified major Prion protein is 27.7 kDa though this protein has several glycosylated forms, may be lipidated, sumoylated and post-translationally cleaved to produce the matrue form of the protein [Uniprot]. NBP2-81078 successfully detected major Prion protein in human brain cerebral cortex, cerebellum, and hippocampus lysates.")
![Immunocytochemistry/Immunofluorescence: PrPSc Antibody (3F4) [NBP2-81078] - Immunofluorescence staining of fixed U251 cells with [NBP2-81078]. Immunofluorescence analysis of paraformaldehyde fixed U251 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 3F4 [NBP2-81078] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81078, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-3F4-Immunocytochemistry-Immunofluorescence-NBP2-81078-img0001.jpg)
![Western Blot: PrPSc Antibody (V21-V) [NBP1-71678] - Western blot analysis of prion protein was performed by Drs. Valeriy Ostapchenko and Marco Prado, Robarts Research Institute, the University of Western Ontario, London, Ontario, Canada. Lanes A-C represent extracts (30 ug of total protein per lane) of PrP-KO CF-10 cells (A), mouse hippocampus (B) and HEK293 cells, transfected with mouse prion protein bearing 3F4 epitope (C); lane D corresponds to 30 ng recombinant MoPrP.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-V21-V-Western-Blot-NBP1-71678-img0006.jpg "Western Blot: PrPSc Antibody (V21-V) [NBP1-71678] - Western blot analysis of prion protein was performed by Drs. Valeriy Ostapchenko and Marco Prado, Robarts Research Institute, the University of Western Ontario, London, Ontario, Canada. Lanes A-C represent extracts (30 ug of total protein per lane) of PrP-KO CF-10 cells (A), mouse hippocampus (B) and HEK293 cells, transfected with mouse prion protein bearing 3F4 epitope (C); lane D corresponds to 30 ng recombinant MoPrP.")
![Western Blot: PrPSc Antibody (V21-V) [NBP1-71678] - Western blot analysis of prion protein in mouse brain extract (A), HEK293 cells producing recombinant human prion protein (B), and untransfected HEK 293 cells (C). 200 ug of total protein loaded per well.](http://images.novusbio.com/fullsize/Prion-protein-Antibody-V21-V-Western-Blot-NBP1-71678-img0005.jpg)