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30 Results for "mfh"

AF6989

FoxC2 Antibody

(26 Publications)

100 μg

AF6989

FoxC2 was detected in immersion fixed frozen sections of mouse embryo (E15.5) using Sheep Anti-Mouse FoxC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6989) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to periocular mesenchyme. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

YAP and TAZ are required for the maintenance of LVs. The lymphatic vessels in the dorsal skin of E16.5 and E18.5 control and Lyve1-Cre;Yapf/f;Tazf/f embryos were analyzed by whole-mount immunohistochemistry. (A,B) LVs were observed in the collecting lymphatic vessels of E16.5 control and Lyve1-Cre;Yapf/f;Tazf/f embryos (arrows). (C,D) The migrating front of E16.5 control (C) and Lyve1-Cre;Yapf/f;Tazf/f (D) embryos appeared comparable. (E-G) At E18.5, the lymphatic vessels from the left and right sides have merged to form a network in control embryos (E). In contrast, huge gaps were observed in between the migrating fronts of E18.5 Lyve1-Cre;Yapf/f;Tazf/f embryos (F, magenta lines). The lymphatic vessels of mutant embryos were also dilated. The distance between the migrating fronts and the diameter of vessels are quantified in G. (H,I) LVs were observed in the collecting lymphatic vessels of E18.5 control embryos (H, yellow arrows). In contrast, the dilated lymphatic vessels of E18.5 Lyve1-Cre;Yapf/f;Tazf/f embryos lacked LVs (I). The various parameters of lymphatic vascular patterning were quantified and are plotted in G. n=4 embryos per each genotype. ****P<0.0001. Data are mean±s.e.m. Scale bars: 200 µm in A-D; 500 µm in E,F; 200 µm in H,I. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33060128), licensed under a CC-BY license. Not internally tested by R&D Systems.

YAP and TAZ are required for the maintenance of LVs. The lymphatic vessels in the dorsal skin of E16.5 and E18.5 control and Lyve1-Cre;Yapf/f;Tazf/f embryos were analyzed by whole-mount immunohistochemistry. (A,B) LVs were observed in the collecting lymphatic vessels of E16.5 control and Lyve1-Cre;Yapf/f;Tazf/f embryos (arrows). (C,D) The migrating front of E16.5 control (C) and Lyve1-Cre;Yapf/f;Tazf/f (D) embryos appeared comparable. (E-G) At E18.5, the lymphatic vessels from the left and right sides have merged to form a network in control embryos (E). In contrast, huge gaps were observed in between the migrating fronts of E18.5 Lyve1-Cre;Yapf/f;Tazf/f embryos (F, magenta lines). The lymphatic vessels of mutant embryos were also dilated. The distance between the migrating fronts and the diameter of vessels are quantified in G. (H,I) LVs were observed in the collecting lymphatic vessels of E18.5 control embryos (H, yellow arrows). In contrast, the dilated lymphatic vessels of E18.5 Lyve1-Cre;Yapf/f;Tazf/f embryos lacked LVs (I). The various parameters of lymphatic vascular patterning were quantified and are plotted in G. n=4 embryos per each genotype. ****P<0.0001. Data are mean±s.e.m. Scale bars: 200 µm in A-D; 500 µm in E,F; 200 µm in H,I. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33060128), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF6989-SP

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AF5044

FoxC2 Antibody [Unconjugated]

(4 Publications)

100 μg

AF5044

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human FoxC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5044) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for FoxC2 was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

FoxC2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human FoxC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5044) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for FoxC2 at approximately 73 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human FoxC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5044) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF5044-SP

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MAB5044

FoxC2 Antibody (665819)

(1 Publication)

100 μg

MAB5044

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, T47D human breast cancer cell line, and MDA-MB-453 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Human FoxC2 Monoclonal Antibody (Catalog # MAB5044) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FoxC2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

FoxC2 was detected in immersion fixed MDA-MB-453 human breast cancer cell line using Human FoxC2 Monoclonal Antibody (Catalog # MAB5044) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB5044-SP

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H00002303-M02

FoxC2 Antibody (2H3)

(6 Publications)

0.1 mg

H00002303-M02

Western Blot: FoxC2 Antibody (2H3) [H00002303-M02] - Western Blot analysis of FOXC2 expression in PC-12 ( Cat # L012V1 ).

Immunocytochemistry/Immunofluorescence: FoxC2 Antibody (2H3) [H00002303-M02] - Analysis of monoclonal antibody to FOXC2 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: FoxC2 Antibody (2H3) [H00002303-M02] - Immunoperoxidase of monoclonal antibody to FoxC2 on formalin-fixed paraffin-embedded human hepatocellular carcinoma. [antibody concentration 3 ug/ml]

Immunohistochemistry-Paraffin: FoxC2 Antibody (2H3) [H00002303-M02] - Analysis of monoclonal antibody to FOXC2 on formalin-fixed paraffin-embedded human lateral ventricle wall. Antibody concentration 3 ug/ml.

Immunohistochemistry-Paraffin: FoxC2 Antibody (2H3) [H00002303-M02] - Immunoperoxidase of monoclonal antibody to FoxC2 on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 3 ug/ml]

Sandwich ELISA: FoxC2 Antibody (2H3) [H00002303-M02] - Detection limit for recombinant GST tagged FOXC2 is approximately 0.3ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NB100-1269

FoxC2 Antibody

(2 Reviews)

(4 Publications)

0.1 mg

NB100-1269

Western Blot: FoxC2 Antibody [NB100-1269] - Staining of nuclear HEK293 cell lysate with antibody at 2 ug/mL (A) and negative control human pancreas cell lysate with antibody at 1 ug/mL (B) (RIPA buffer 35 ug total protein per lane). Detected by chemiluminescence.

Flow Cytometry: FoxC2 Antibody [NB100-1269] - Analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Reactivity:

Hu, Mu, Rt, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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H00002303-M04

FoxC2 Antibody (1A8)

(1 Publication)

0.1 mg

H00002303-M04

Western Blot: FoxC2 Antibody (1A8) [H00002303-M04] - Analysis of FOXC2 expression in NIH/3T3.

Western Blot: FoxC2 Antibody (1A8) [H00002303-M04] - FOXC2 monoclonal antibody (M04), clone 1A8. Western Blot analysis of FOXC2 expression in PC-12(Cat # L012V1 ).

Western Blot: FoxC2 Antibody (1A8) [H00002303-M04] - FOXC2 monoclonal antibody (M04), clone 1A8 Western Blot analysis of FOXC2 expression in K-562 ( Cat # L009V1 ).

Western Blot: FoxC2 Antibody (1A8) [H00002303-M04] - Analysis of FOXC2 expression in 293.

Sandwich ELISA: FoxC2 Antibody (1A8) [H00002303-M04] - Detection limit for recombinant GST tagged FOXC2 is 3 ng/ml as a capture antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00002303-M16

FoxC2 Antibody (1D4)

(1 Publication)

50 μg

H00002303-M16

Immunocytochemistry/Immunofluorescence: FoxC2 Antibody (1D4) [H00002303-M16] - Analysis of monoclonal antibody to FOXC2 on HeLa cell. Antibody concentration 10 ug/ml

Sandwich ELISA: FoxC2 Antibody (1D4) [H00002303-M16] - Detection limit for recombinant GST tagged FOXC2 is 0.1 ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00002303-M08

FoxC2 Antibody (3E6)

0.1 mg

H00002303-M08

Western Blot: FoxC2 Antibody (3E6) [H00002303-M08] - Analysis of FOXC2 expression in Jurkat (Cat # L017V1).

Western Blot: FoxC2 Antibody (3E6) [H00002303-M08] - Analysis of FOXC2 expression in PC-12 (Cat # L012V1).

Sandwich ELISA: FoxC2 Antibody (3E6) [H00002303-M08] - Detection limit for recombinant GST tagged FOXC2 is 1 ng/ml as a capture antibody.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00002303-M05

FoxC2 Antibody (3H5)

0.1 mg

H00002303-M05

Western Blot: FoxC2 Antibody (3H5) [H00002303-M05] - Analysis of FOXC2 expression in Jurkat (Cat # L017V1).

Western Blot: FoxC2 Antibody (3H5) [H00002303-M05] - Analysis of FOXC2 expression in PC-12 (Cat # L012V1).

Sandwich ELISA: FoxC2 Antibody (3H5) [H00002303-M05] - Detection limit for recombinant GST tagged FOXC2 is 3 ng/ml as a capture antibody.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-45710

FoxC2 Antibody (OTI3E3)

0.1 ml

NBP2-45710

Western Blot: FOXC2 Antibody (3E3) [NBP2-45710] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXC2.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Mouse monoclonal

Host: Mouse monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-70752AF350
Alexa Fluor 405	NBP2-70752AF405
Alexa Fluor 488	NBP2-70752AF488
Alexa Fluor 532	NBP2-70752AF532
Alexa Fluor 594	NBP2-70752AF594
Alexa Fluor 647	NBP2-70752AF647
Alexa Fluor 700	NBP2-70752AF700
Alexa Fluor 750	NBP2-70752AF750
Allophycocyanin	NBP2-70752APC
Biotin	NBP2-70752B
CoraFluor 1	NBP2-70752CL1
DyLight 350	NBP2-70752UV
DyLight 405	NBP2-70752V
DyLight 488	NBP2-70752G
DyLight 550	NBP2-70752R
DyLight 594	NBP2-70752DL594
DyLight 650	NBP2-70752C
DyLight 680	NBP2-70752FR
DyLight 755	NBP2-70752IR
FITC	NBP2-70752F
HRP	NBP2-70752H
Janelia Fluor 525	NBP2-70752JF525
Janelia Fluor 585	NBP2-70752JF585
Janelia Fluor 635	NBP2-70752JF635
Janelia Fluor 669	NBP2-70752JF669
PE	NBP2-70752PE
PerCP	NBP2-70752PCP

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-70752

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NBP2-37409

FoxC2 Antibody (1D11C8) - BSA Free

0.1 ml

NBP2-37409

Western Blot: FoxC2 Antibody (1D11C8) [NBP2-37409] - Western blot analysis using FOXC2 mAb against human FOXC2 recombinant protein. (Expected MW is 47.2 kDa)

ELISA: FoxC2 Antibody (1D11C8) [NBP2-37409] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-37409-0.025ml

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H00002303-P01

Recombinant Human FoxC2 GST (N-Term) Protein

10 μg

H00002303-P01

SDS-Page: Recombinant Human FoxC2 Protein [H00002303-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

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