![Simple Western: Cytokeratin 19 Antibody [NB100-687] - Lane view shows a specific band for Cytokeratin 19 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under standard reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Simple-Western-NB100-687-img0008.jpg "Simple Western: Cytokeratin 19 Antibody [NB100-687] - Lane view shows a specific band for Cytokeratin 19 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under standard reducing conditions using the 12-230 kDa separation system.")
| Reactivity | Hu, Mu, Rt, Pm-OrSpecies Glossary |
| Applications | WB, Simple Western, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Synthetic peptide derived from the C-terminal region of human Cytokeratin 19 (between residues 350-400) [UniProt P08727] |
| Localization | Cytoplasmic. |
| Marker | Epithelial Cell Marker |
| Predicted Species | Orangutan (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | KRT19 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This Cytokeratin 19 antibody is useful for Western Blot where a band is seen ~44 kDa, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry-paraffin sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS and 30% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
IHC-P | Mouse | 03/04/2009 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 19 Antibody (NB100-687)Find related products by research area.
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![Western Blot: Cytokeratin 19 Antibody [NB100-687] - Western blot detection of YAP and TAZ in proteins extracted from isolated hepatocytes and cholangiocytes and total liver tissue. Antibodies against CK19 and HNF4a were used to confirm purity of the populations and b-actin was used as a loading control. Representative results from a single experiment with n = 3 independent cell isolations and n = 2 total liver tissues. Image collected and cropped by CiteAb from the following publication(//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Western-Blot-NB100-687-img0010.jpg "Western Blot: Cytokeratin 19 Antibody [NB100-687] - Western blot detection of YAP and TAZ in proteins extracted from isolated hepatocytes and cholangiocytes and total liver tissue. Antibodies against CK19 and HNF4a were used to confirm purity of the populations and b-actin was used as a loading control. Representative results from a single experiment with n = 3 independent cell isolations and n = 2 total liver tissues. Image collected and cropped by CiteAb from the following publication(//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - TUDCA induces intermediate states of mesenchymal and epithelial cells in primary cultures of rat HSC during hepatic differentiation. Under control conditions HSC remained negative for K19 (Cytokeratin 19) expression, (d) but this epithelial marker protein was induced in HSC cultures after treatment with 2 uM TUDCA for 14 days (red). (f) K19 was found to be co-expressed with desmin (green), which indicated the origin of epithelial progenitor cells from HSC. (I) Cells with different states of maturation were still found after 21 days of TUDCA treatment. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep13320), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Immunocytochemistry-Immunofluorescence-NB100-687-img0013.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - TUDCA induces intermediate states of mesenchymal and epithelial cells in primary cultures of rat HSC during hepatic differentiation. Under control conditions HSC remained negative for K19 (Cytokeratin 19) expression, (d) but this epithelial marker protein was induced in HSC cultures after treatment with 2 uM TUDCA for 14 days (red). (f) K19 was found to be co-expressed with desmin (green), which indicated the origin of epithelial progenitor cells from HSC. (I) Cells with different states of maturation were still found after 21 days of TUDCA treatment. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep13320), licensed under a CC-BY license.")
![Immunohistochemistry: Cytokeratin 19 Antibody [NB100-687] - Inhibition of MST improves liver regeneration in aged mice. Representative photomicrograph of an aged liver treated with siMST and stained with H&E and by IHC for CK19 and Ki67. Arrows indicate ductal regions with no signs of reaction or oval cell expansion. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Immunohistochemistry-NB100-687-img0012.jpg "Immunohistochemistry: Cytokeratin 19 Antibody [NB100-687] - Inhibition of MST improves liver regeneration in aged mice. Representative photomicrograph of an aged liver treated with siMST and stained with H&E and by IHC for CK19 and Ki67. Arrows indicate ductal regions with no signs of reaction or oval cell expansion. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - Analysis of Cytokeratin 19 in MCF7 cells using Cytokeratin 19 antibody (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Immunocytochemistry-Immunofluorescence-NB100-687-img0004.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - Analysis of Cytokeratin 19 in MCF7 cells using Cytokeratin 19 antibody (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Flow Cytometry: Cytokeratin 19 Antibody [NB100-687] - An intracellular stain was performed on MCF7 cells with Cytokeratin 19 Antibody NB100-687AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Flow-Cytometry-NB100-687-img0009.jpg "Flow Cytometry: Cytokeratin 19 Antibody [NB100-687] - An intracellular stain was performed on MCF7 cells with Cytokeratin 19 Antibody NB100-687AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Western Blot: Cytokeratin 19 Antibody [NB100-687] - Analysis of Cytokeratin 19 in 1) HepG2 and 2) MCF7 lysates.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Western-Blot-NB100-687-img0006.jpg "Western Blot: Cytokeratin 19 Antibody [NB100-687] - Analysis of Cytokeratin 19 in 1) HepG2 and 2) MCF7 lysates.")
![Immunohistochemistry: Cytokeratin 19 Antibody [NB100-687] - Staining of Cytokeratin 19 in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-Immunohistochemistry-NB100-687-img0007.jpg "Immunohistochemistry: Cytokeratin 19 Antibody [NB100-687] - Staining of Cytokeratin 19 in human kidney carcinoma using DAB with hematoxylin counterstain.")
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody - BSA Free [NB100-687] - TUDCA induces intermediate states of mesenchymal & epithelial cells in primary cultures of rat HSC during hepatic differentiation.Freshly isolated rat HSC (a) exhibited typical lipid droplets that contained retinoids & (b) expressed the mesenchymal filament proteins vimentin (red) & desmin (green) as investigated by immunofluorescence. (c) Under control conditions HSC remained negative for K19 expression, (d) but this epithelial marker protein was induced in HSC cultures after treatment with 2 μM TUDCA for 14 days (red). (e–g) K19 was found to be co-expressed with desmin & vimentin (green), which indicated the origin of epithelial progenitor cells from HSC. (h–k) Also K18, Afp & Mrp2 (red), which served as markers for hepatic differentiation, were co-expressed with vimentin & desmin (green) after 21 days of TUDCA treatment. Cells with different states of maturation were still found after 21 days of TUDCA treatment. Some cells developed into hepatocyte-like cells with (j) dominant K18 filaments (white arrows) while others remained immature with persisting desmin (red arrow) or (l) K19 protein residues. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep13320), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-687_rabbit-polyclonal-cytokeratin-19-antibody-31020241683916.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody - BSA Free [NB100-687] - TUDCA induces intermediate states of mesenchymal & epithelial cells in primary cultures of rat HSC during hepatic differentiation.Freshly isolated rat HSC (a) exhibited typical lipid droplets that contained retinoids & (b) expressed the mesenchymal filament proteins vimentin (red) & desmin (green) as investigated by immunofluorescence. (c) Under control conditions HSC remained negative for K19 expression, (d) but this epithelial marker protein was induced in HSC cultures after treatment with 2 μM TUDCA for 14 days (red). (e–g) K19 was found to be co-expressed with desmin & vimentin (green), which indicated the origin of epithelial progenitor cells from HSC. (h–k) Also K18, Afp & Mrp2 (red), which served as markers for hepatic differentiation, were co-expressed with vimentin & desmin (green) after 21 days of TUDCA treatment. Cells with different states of maturation were still found after 21 days of TUDCA treatment. Some cells developed into hepatocyte-like cells with (j) dominant K18 filaments (white arrows) while others remained immature with persisting desmin (red arrow) or (l) K19 protein residues. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep13320), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")