![Western Blot: Collagen X alpha 1 Antibody (JF0961) [NBP2-66988] - Analysis of Collagen X alpha 1 on rat liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Collagen-X-alpha-1-Antibody-JF0961-Western-Blot-NBP2-66988-img0002.jpg "Western Blot: Collagen X alpha 1 Antibody (JF0961) [NBP2-66988] - Analysis of Collagen X alpha 1 on rat liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB |
| Clone | JF0961 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human Collagen X alpha 1 aa 550-599 / 680. (SwissProt: Q03692 Human; SwissProt: Q05306 Mouse; Unigene:20579 Rat) |
| Localization | Extracellular matrix. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
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![Western Blot: Collagen X alpha 1 Antibody (JF0961) [NBP2-66988] - Analysis of Collagen X alpha 1 on human liver tissue lysates with Rabbit anti-Collagen X alpha 1 antibody at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Collagen-X-alpha-1-Antibody-JF0961-Western-Blot-NBP2-66988-img0003.jpg "Western Blot: Collagen X alpha 1 Antibody (JF0961) [NBP2-66988] - Analysis of Collagen X alpha 1 on human liver tissue lysates with Rabbit anti-Collagen X alpha 1 antibody at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
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