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Chk2 [p Thr68] Antibody [Unconjugated]

 (9 Publications)
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-Chk2 (T68) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1626) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Chk2 (T68) was detected at approximately 64 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 60 minutes. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human ...read more
Phopsho-Chk2 (T68) was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line treated with 1 µM camptothecin using Rabbit Anti-Human Phospho-Chk2 (T68) Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF1626-SP
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Chk2 [p Thr68] Antibody [Unconjugated] Summary

Immunogen
Phosphopeptide containing human Chk2 T68 site
Modification
p Thr68
Specificity
Detects human Chk2 when phosphorylated at T68.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHEK2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF1626 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chk2 [p Thr68] Antibody [Unconjugated]

  • CDS1
  • CHEK2
  • CHK2 checkpoint homolog (S. pombe)
  • Chk2
  • EC 2.7.11
  • EC 2.7.11.1
  • HuCds1
  • LFS2
  • PP1425
  • Rad53
  • S.pombe) homolog

Background

ATM phosphorylates the Checkpoint kinase on T68 in response to genotoxic insults. Phosphorylation of T68 promotes the formation of Chk2 oligomers. Oligomerization is a critical event in the activation of the Chk2 kinase as it is required for phosphorylation of additional Chk2 residues that lead to full activation and signaling to downstream effectors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Chk2 [p Thr68] Antibody [Unconjugated]
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Publications for Chk2 Antibody (AF1626)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC, Western Blot.


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ICC
(1)
Western Blot
(7)
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Human
(7)
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Showing Publications 1 - 9 of 9.
Publications using AF1626 Applications Species
Boichuk S, Bikinieva F, Valeeva E et al. Establishment and Characterization of Multi-Drug Resistant p53-Negative Osteosarcoma SaOS-2 Subline Diagnostics (Basel) 2023-08-11 [PMID: 37627905]
Goutham Narayanan Subramanian, Jessica Greaney, Zhe Wei, Olivier Becherel, Martin Lavin, Hayden Anthony Homer Oocytes mount a noncanonical DNA damage response involving APC-Cdh1–mediated proteolysis Journal of Cell Biology 2020-04-06 [PMID: 32328643]
EM Chan, T Shibue, JM McFarland, B Gaeta, M Ghandi, N Dumont, A Gonzalez, JS McPartlan, T Li, Y Zhang, J Bin Liu, JB Lazaro, P Gu, CG Piett, A Apffel, SO Ali, R Deasy, P Keskula, RWS Ng, EA Roberts, E Reznichenk, L Leung, M Alimova, M Schenone, M Islam, YE Maruvka, Y Liu, J Roper, S Raghavan, M Giannakis, YY Tseng, ZD Nagel, A D'Andrea, DE Root, JS Boehm, G Getz, S Chang, TR Golub, A Tsherniak, F Vazquez, AJ Bass WRN helicase is a synthetic lethal target in microsatellite unstable cancers Nature, 2019-04-10;568(7753):551-556. 2019-04-10 [PMID: 30971823] (ICC, Western Blot, Human) ICC, Western Blot Human
Shi Y, Felley-Bosco E, Marti T, Orlowski K, Pruschy M, Stahel R Starvation-induced activation of ATM/Chk2/p53 signaling sensitizes cancer cells to cisplatin. BMC Cancer, 2012-12-04;12(0):571. 2012-12-04 [PMID: 23211021] (Western Blot, Human) Western Blot Human
Boichuk S, Hu L, Hein J Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen. J. Virol., 2010-06-02;84(16):8007-20. 2010-06-02 [PMID: 20519379] (Western Blot, Human) Western Blot Human
Badie S, Liao C, Thanasoula M, Barber P, Hill MA, Tarsounas M RAD51C facilitates checkpoint signaling by promoting CHK2 phosphorylation. J. Cell Biol., 2009-05-18;185(4):587-600. 2009-05-18 [PMID: 19451272] (Western Blot, Human) Western Blot Human
Leonard JM, Ye H, Wetmore C, Karnitz LM Sonic Hedgehog signaling impairs ionizing radiation-induced checkpoint activation and induces genomic instability. J. Cell Biol., 2008-10-27;183(3):385-91. 2008-10-27 [PMID: 18955550] (Western Blot, Human) Western Blot Human
Hein J, Boichuk S, Wu J, Cheng Y, Freire R, Jat PS, Roberts TM, Gjoerup OV Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding. J. Virol., 2008-10-15;83(1):117-27. 2008-10-15 [PMID: 18922873] (Western Blot, Human) Western Blot Human
Sakasai R, Tibbetts R RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage. J. Biol. Chem., 2008-03-12;283(20):13549-55. 2008-03-12 [PMID: 18337245] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol CHEK2