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CD117/c-kit Antibody [Unconjugated]

Name: CD117/c-kit Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF1356
Rating: 4.3 (4 reviews)

(110 Publications)

(4 Reviews)

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CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. ...read more

Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9-2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse ...read more

Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit ...read more

Localization of MAFB in mouse testes.(A) Localization of MAFB in E18.5 mouse testes. Double immunostaining of MAFB with E-cadherin (ECAD), GATA4, or STAR is shown. Nuclei were counterstained with DAPI. The color of each ...read more

Spermatogenesis development of adult Mafb-cKO mice.Six-week-old mice were injected with tamoxifen and testes of cKO mice at 3- and 8-months of age were examined compared to those of age-matched controls (n = 4 for each ...read more

Product Details

Summary

Reactivity	Hu, MuSpecies Glossary
Applications	WB, Simple Western, Flow, IHC, CyTOF-ready, Dual ISH-IHC
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

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Biotin BAF1356

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Carrier Free AF1356-SP

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Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF1356

CD117/c-kit Antibody [Unconjugated] Summary

Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit Gln25-Thr519 (Ala207Glu) Accession # P05532
Specificity	Detects human and mouse CD117/c-kit in direct ELISAs and Western blots.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	KIT
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

CyTOF-ready
Dual RNAscope ISH-IHC 5-15 ug/mL
Flow Cytometry 2.5 ug/10^6 cells
Immunohistochemistry 5-15 ug/mL
Simple Western 5 ug/mL
Western Blot 0.1 ug/mL

Reviewed Applications

Read 4 Reviews rated 4.3

using
AF1356 in the following applications:

Western Blot
Immunofluorescence
Immunocytochemistry

Publications

Read Publications using
AF1356 in the following applications:

Flow Cytometry
5 publications
ICC
10 publications
ICC/IF
1 publication
IF
1 publication
IHC
32 publications
IHC-Fr
2 publications
IHC-P
7 publications
Neutralization
1 publication
Simple Western
1 publication
Western Blot
9 publications

Packaging, Storage & Formulations

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD117/c-kit Antibody [Unconjugated]

CD117 antigen
CD117
ckit
c-kit
EC 2.7.10
EC 2.7.10.1
KIT
PBT
piebald trait
Proto-oncogene c-Kit
proto-oncogene tyrosine-protein kinase Kit
SCF R
SCFR
SCFRmast/stem cell growth factor receptor
soluble KIT variant 1
Tyrosine-protein kinase Kit
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD117/c-kit Antibody (AF1356)(110)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Porcine, Transgenic Mouse.

We have publications tested in 10 applications: Flow Cytometry, ICC, ICC/IF, IF, IHC, IHC-Fr, IHC-P, Neutralization, Simple Western, Western Blot.

Showing Publications 1 - 10 of 110. Show All 110 Publications.

Publications using AF1356	Applications	Species
Iga, T;Kobayashi, H;Kusumoto, D;Sanosaka, T;Fujita, N;Tai-Nagara, I;Ando, T;Takahashi, T;Matsuo, K;Hozumi, K;Ito, K;Ema, M;Miyamoto, T;Matsumoto, M;Nakamura, M;Okano, H;Shibata, S;Kohyama, J;Kim, KK;Takubo, K;Kubota, Y; Spatial heterogeneity of bone marrow endothelial cells unveils a distinct subtype in the epiphysis Nature cell biology 2023-10-05 [PMID: 37798545] (IHC, Mouse)	IHC	Mouse
Lei, WL;Li, YY;Du, Z;Su, R;Meng, TG;Ning, Y;Hou, G;Schatten, H;Wang, ZB;Han, Z;Sun, F;Qian, WP;Liu, C;Sun, QY; SRSF1-mediated alternative splicing is required for spermatogenesis International journal of biological sciences 2023-09-11 [PMID: 37781512] (IHC, Mouse)	IHC	Mouse
Li X, Ji S et al. Adeno-associated virus-9 reverses delayed gastric emptying of solids in diabetic mice. Neurogastroenterol Motil 2024-09-13 [PMID: 37702100] (Simple Western, Mouse) Details: 1:1000	Simple Western	Mouse
Ru-pin Alicia Chi, Xiaojiang Xu, Jian-Liang Li, Xin Xu, Guang Hu, Paula Brown et al. WNK1 is required during male pachynema to sustain fertility iScience 9/15/2023 [PMID: 37694147]
Elodie Grockowiak, Claudia Korn, Justyna Rak, Veronika Lysenko, Adrien Hallou, Francesca M. Panvini et al. Different niches for stem cells carrying the same oncogenic driver affect pathogenesis and therapy response in myeloproliferative neoplasms Nature Cancer 8/7/2023 [PMID: 37550517]
Hansen, SL;Larsen, HL;Pikkupeura, LM;Maciag, G;Guiu, J;Müller, I;Clement, DL;Mueller, C;Johansen, JV;Helin, K;Lerdrup, M;Jensen, KB; An organoid-based CRISPR-Cas9 screen for regulators of intestinal epithelial maturation and cell fate Science advances 2023-07-14 [PMID: 37436979] (IHC, Mouse)	IHC	Mouse
Zhang, X;Liu, Y;Sosa, F;Gunewardena, S;Crawford, PA;Zielen, AC;Orwig, KE;Wang, N; Transcriptional metabolic reprogramming implements meiotic fate decision in mouse testicular germ cells Cell reports 2023-07-04 [PMID: 37405912] (ICC/IF, IHC, Western Blot, Mouse)	ICC/IF, IHC, Western Blot	Mouse
Liu C, Wang L, Li Y et al. RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis Nucleic acids research 6/28/2023 [PMID: 37378420]
Nguyen, VTT;Taheri, N;Choi, EL;Kellogg, TA;Linden, DR;Hayashi, Y; Insulin-like growth factor1 preserves gastric pacemaker cells and motor function in aging via ERK1/2 activation Cellular and molecular gastroenterology and hepatology 2023-06-08 [PMID: 37301443] (IHC, Western Blot, Mouse)	IHC, Western Blot	Mouse
Nakajima-Takagi, Y;Oshima, M;Takano, J;Koide, S;Itokawa, N;Uemura, S;Yamashita, M;Andoh, S;Aoyama, K;Isshiki, Y;Shinoda, D;Saraya, A;Arai, F;Yamaguchi, K;Furukawa, Y;Koseki, H;Ikawa, T;Iwama, A; Polycomb repressive complex 1.1 coordinates homeostatic and emergency myelopoiesis eLife 2023-06-02 [PMID: 37266576] (IHC, Transgenic Mouse, Mouse)	IHC	Transgenic Mouse, Mouse
Hao, X;Shen, Y;Chen, N;Zhang, W;Valverde, E;Wu, L;Chan, HL;Xu, Z;Yu, L;Gao, Y;Bado, I;Michie, LN;Rivas, CH;Dominguez, LB;Aguirre, S;Pingel, BC;Wu, YH;Liu, F;Ding, Y;Edwards, DG;Liu, J;Alexander, A;Ueno, NT;Hsueh, PR;Tu, CY;Liu, LC;Chen, SH;Hung, MC;Lim, B;Zhang, XH; Osteoprogenitor-GMP crosstalk underpins solid tumor-induced systemic immunosuppression and persists after tumor removal Cell stem cell 2023-05-04 [PMID: 37146584] (IHC, Mouse)	IHC	Mouse
Yi Jacky Peng, Xinyu Thomas Tang, Hui Sophie Shu, Wenjie Dong, Hongfang Shao, Bo O. Zhou Sertoli cells are the unique source of stem cell factor for spermatogenesis Development 3/15/2023 [PMID: 36861441]
A Uchida, K Imaimatsu, H Suzuki, X Han, H Ushioda, M Uemura, K Imura-Kish, R Hiramatsu, HM Takase, Y Hirate, A Ogura, M Kanai-Azum, A Kudo, Y Kanai SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse Nature Communications, 2022-12-21;13(1):7860. 2022-12-21 [PMID: 36543770] (IHC, Mouse)	IHC	Mouse
T Klein-Rode, K Micklich, A Sanz-Moren, M Tost, J Calzada-Wa, T Adler, M Klaften, S Sabrautzki, B Aigner, M Kraiger, V Gailus-Dur, H Fuchs, German Mou, A Gründer, H Pahl, E Wolf, M Hrabe de A, B Rathkolb New C3H KitN824K/WT cancer mouse model develops late-onset malignant mammary tumors with high penetrance Scientific Reports, 2022-11-17;12(1):19793. 2022-11-17 [PMID: 36396684] (IHC, Mouse)	IHC	Mouse
Z Zheng, H He, XT Tang, H Zhang, F Gou, H Yang, J Cao, S Shi, Z Yang, G Sun, X Xie, Y Zeng, A Wen, Y Lan, J Zhou, B Liu, BO Zhou, T Cheng, H Cheng Uncovering the emergence of HSCs in the human fetal bone marrow by single-cell RNA-seq analysis Cell Stem Cell, 2022-11-03;29(11):1562-1579.e7. 2022-11-03 [PMID: 36332570] (Flow Cytometry, Human)	Flow Cytometry	Human
Oleksandr Kirsanov, Taylor Johnson, Taylor Malachowski, Bryan A. Niedenberger, Emma A. Gilbert, Debajit Bhowmick et al. Modeling mammalian spermatogonial differentiation and meiotic initiation in vitro Development 11/15/2022 [PMID: 36250451]
Peart NJ, Johnson TA, Lee S et al. The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice PLOS Genetics 9/21/2022 [PMID: 36129965]
Rahul Mallick, Erika Gurzeler, Pyry I. Toivanen, Tiina Nieminen, Seppo Ylä-Herttuala Novel Designed Proteolytically Resistant VEGF-B186R127S Promotes Angiogenesis in Mouse Heart by Recruiting Endothelial Progenitor Cells Frontiers in Bioengineering and Biotechnology 8/4/2022 [PMID: 35992336]
Lixin Wang, Pu-Qing Yuan, Collin Challis, Sripriya Ravindra Kumar, Yvette Taché Transduction of Systemically Administered Adeno-Associated Virus in the Colonic Enteric Nervous System and c-Kit Cells of Adult Mice Frontiers in Neuroanatomy 6/6/2022 [PMID: 35734536]
Sarah C. Moody, Penny A. F. Whiley, Patrick S. Western, Kate L. Loveland The Impact of Activin A on Fetal Gonocytes: Chronic Versus Acute Exposure Outcomes Front Endocrinol (Lausanne) 5/31/2022 [PMID: 35721752]
Hirano K, Nonami Y, Nakamura Y et al. Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis Communications Biology 5/26/2022 [PMID: 35618762]
Xi Chen, Shanshan Li, Xiaoli Liu, Jingjie Zhao, Lanting Wu, Ran You et al. Stimulation of C-Kit+ Retinal Progenitor Cells by Stem Cell Factor Confers Protection Against Retinal Degeneration Frontiers in Pharmacology 3/31/2022 [PMID: 35431956]
S Klingler, KS Hsu, G Hua, ML Martin, M Adileh, T Baslan, Z Zhang, PB Paty, Z Fuks, AM Brown, R Kolesnick Disruption of the crypt niche promotes outgrowth of mutated colorectal tumor stem cells JCI Insight, 2022-03-08;7(5):. 2022-03-08 [PMID: 35260534] (IHC, Mouse)	IHC	Mouse
J Heil, V Olsavszky, K Busch, K Klapproth, C de la Torr, C Sticht, K Sandorski, J Hoffmann, H Schönhaber, J Zierow, M Winkler, CD Schmid, T Staniczek, DE Daniels, J Frayne, G Metzgeroth, D Nowak, S Schneider, M Neumaier, V Weyer, C Groden, HJ Gröne, K Richter, C Mogler, MM Taketo, K Schledzews, C Géraud, S Goerdt, PS Koch Bone marrow sinusoidal endothelium controls terminal erythroid differentiation and reticulocyte maturation Nature Communications, 2021-11-29;12(1):6963. 2021-11-29 [PMID: 34845225] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
IC Young, B Wu, J Andricovic, ST Chuang, R Li, A Tzatsos, RC Wu, MY Wu Differentiation of fetal hematopoietic stem cells requires ARID4B to restrict autocrine KITLG/KIT-Src signaling Cell Reports, 2021-11-23;37(8):110036. 2021-11-23 [PMID: 34818550] (Western Blot, Human)	Western Blot	Human
A Heinrich, B Bhandary, SJ Potter, N Ratner, T DeFalco Cdc42 activity in Sertoli cells is essential for maintenance of spermatogenesis Cell Reports, 2021-10-26;37(4):109885. 2021-10-26 [PMID: 34706238] (IHC, Mouse)	IHC	Mouse
F Progatzky, M Shapiro, SH Chng, B Garcia-Cas, CH Classon, S Sevgi, A Laddach, AC Bon-Frauch, R Lasrado, M Rahim, EM Amaniti, S Boeing, K Shah, LJ Entwistle, A Suárez-Bon, MS Wilson, B Stockinger, V Pachnis Regulation of intestinal immunity and tissue repair by enteric glia Nature, 2021-10-20;599(7883):125-130. 2021-10-20 [PMID: 34671159] (IHC, Mouse)	IHC	Mouse
K Kimura, M Breitbach, FA Schildberg, M Hesse, BK Fleischman Bone marrow CD73+ mesenchymal stem cells display increased stemness in vitro and promote fracture healing in vivo Bone Reports, 2021-09-29;15(0):101133. 2021-09-29 [PMID: 34632004] (IHC, Mouse)	IHC	Mouse
Seung-Ju Hwang, Jing-Hua Wang, Jin-Seok Lee, Hwa-Dong Lee, Tae-Joon Choi, Seo-Hyung Choi et al. Yeokwisan, a Standardized Herbal Formula, Enhances Gastric Emptying via Modulation of the Ghrelin Pathway in a Loperamide-induced Functional Dyspepsia Mouse Model Frontiers in Pharmacology 9/22/2021 [PMID: 34630123]
T Aschacher, K Schmidt, O Aschacher, E Eichmair, U Baranyi, B Winkler, M Grabenwoeg, A Spittler, F Enzmann, B Messner, J Riebandt, G Laufer, M Bergmann, M Ehrlich Telocytes in the human ascending aorta: Characterization and exosome-related KLF-4/VEGF-A expression Journal of Cellular and Molecular Medicine, 2021-09-25;0(0):. 2021-09-25 [PMID: 34562312] (Flow Cytometry, Human)	Flow Cytometry	Human
Azzurra Codino, Tomasz Turowski, Louie N. van de Lagemaat, Ivayla Ivanova, Andrea Tavosanis, Christian Much et al. NANOS2 is a sequence-specific mRNA-binding protein that promotes transcript degradation in spermatogonial stem cells iScience 7/23/2021 [PMID: 34278268]
Rivero-Hinojosa S, Pugacheva EM, Kang S et al. The combined action of CTCF and its testis-specific paralog BORIS is essential for spermatogenesis Nature Communications 6/22/2021 [PMID: 34158481]
Jelinkova S, Sleiman Y, FojtIk P et al. Dystrophin Deficiency Causes Progressive Depletion of Cardiovascular Progenitor Cells in the Heart International Journal of Molecular Sciences 5/10/2021 [PMID: 34068508]
K Matsumoto, M Suenaga, Y Mizutani, K Matsui, A Yoshida, T Nakamoto, S Kato Role of transient receptor potential vanilloid subtype 2 in lower oesophageal sphincter in rat acid reflux oesophagitis Journal of pharmacological sciences, 2021-04-07;146(3):125-135. 2021-04-07 [PMID: 34030795] (IHC, Rat)	IHC	Rat
Sang-woo Lee, Junchul Kim, Xin Cong, Guang-Yan Yu, Ji Hyun Ryu, Kyungpyo Park Efficient Surface Immobilization of Chemically Modified Hyaluronans for Enhanced Bioactivity and Survival of In Vitro-Cultured Embryonic Salivary Gland Mesenchymal Cells Polymers (Basel) 4/9/2021 [PMID: 33918722]
Okiko Habara, Catriona Y. Logan, Masami Kanai-Azuma, Roeland Nusse, Hinako M. Takase WNT signaling in pre-granulosa cells is required for ovarian folliculogenesis and female fertility Development 5/1/2021 [PMID: 33914868]
W Jia, L Kong, H Kidoya, H Naito, F Muramatsu, Y Hayashi, HY Hsieh, D Yamakawa, DK Hsu, FT Liu, N Takakura Indispensable role of Galectin-3 in promoting quiescence of hematopoietic stem cells Nature Communications, 2021-04-09;12(1):2118. 2021-04-09 [PMID: 33837181] (IHC, Mouse)	IHC	Mouse
Shinnosuke Suzuki, John R. McCarrey, Brian P. Hermann Differential RA responsiveness among subsets of mouse late progenitor spermatogonia Reproduction 6/1/2021 [PMID: 33835049]
S Suzuki, JR McCarrey, BP Hermann An mTORC1-dependent switch orchestrates the transition between mouse spermatogonial stem cells and clones of progenitor spermatogonia Cell Reports, 2021-02-16;34(7):108752. 2021-02-16 [PMID: 33596419] (Flow Cytometry, ICC, Mouse)	Flow Cytometry, ICC	Mouse
Sarah Guimbal, Lauriane Cornuault, Paul Rouault, Pierre-Louis Hollier, Candice Chapouly, Marie-Lise Bats et al. Mast Cells Are the Trigger of Small Vessel Disease and Diastolic Dysfunction in Diabetic Obese Mice Arteriosclerosis, Thrombosis, and Vascular Biology 4/1/2021 [PMID: 33567863]
K Imura-Kish, A Uchida, N Tsunekawa, H Suzuki, HM Takase, Y Hirate, M Kanai-Azum, R Hiramatsu, M Kurohmaru, Y Kanai Low retinoic acid levels mediate regionalization of the Sertoli valve in the terminal segment of mouse seminiferous tubules Scientific Reports, 2021-01-13;11(1):1110. 2021-01-13 [PMID: 33441739] (IHC, Mouse)	IHC	Mouse
Salah A Baker, Wesley A Leigh, Guillermo Del Valle, Inigo F De Yturriaga, Sean M Ward, Caroline A Cobine et al. Ca2+ signaling driving pacemaker activity in submucosal interstitial cells of Cajal in the murine colon eLife 1/5/2021 [PMID: 33399536]
Lifei Hou, Richard A. Voit, Vijay G. Sankaran, Timothy A. Springer, Koichi Yuki CD11c regulates hematopoietic stem and progenitor cells under stress Blood Advances 12/22/2020 [PMID: 33351105]
T Su, Y Yang, S Lai, J Jeong, Y Jung, M McConnell, T Utsumi, Y Iwakiri Single-cell transcriptomics reveals zone-specific alterations of liver sinusoidal endothelial cells in cirrhosis Cellular and Molecular Gastroenterology and Hepatology, 2020-12-16;0(0):. 2020-12-16 [PMID: 33340713] (IHC, Mouse)	IHC	Mouse
Iromi Wanigasuriya, Quentin Gouil, Sarah A Kinkel, Andrés Tapia del Fierro, Tamara Beck, Ellise A Roper et al. Smchd1 is a maternal effect gene required for genomic imprinting eLife 11/13/2020 [PMID: 33186096]
N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer Nat Commun, 2020-10-14;11(1):5171. 2020-10-14 [PMID: 33057002] (IHC, Western Blot, Mouse)	IHC, Western Blot	Mouse
EI Crosse, S Gordon-Key, S Rybtsov, A Binagui-Ca, H Felchle, NC Nnadi, K Kirschner, T Chandra, S Tamagno, DJ Webb, F Rossi, RA Anderson, A Medvinsky Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development Cell Stem Cell, 2020-09-17;0(0):. 2020-09-17 [PMID: 32946788] (IHC, Human)	IHC	Human
C Sogawa-Fuj, A Hanagata, Y Fujiwara, Y Ishida, H Tomiyasu, T Kunieda, H Nakatomi, M Hori Defective development and microcirculation of intestine in Npr2 mutant mice Sci Rep, 2020-09-08;10(1):14761. 2020-09-08 [PMID: 32901096] (IHC, Western Blot, Mouse)	IHC, Western Blot	Mouse
DE Ebeid, F Firouzi, CY Esquer, JM Navarrete, BJ Wang, NA Gude, MA Sussman PIM1 Promotes Survival of Cardiomyocytes by Upregulating c-Kit Protein Expression Cells, 2020-08-31;9(9):. 2020-08-31 [PMID: 32878131] (Western Blot, Mouse)	Western Blot	Mouse
Y Hayashi, DT Asuzu, MR Bardsley, GB Gajdos, SM Kvasha, DR Linden, RA Nagy, SA Saravanape, SA Syed, Y Toyomasu, H Yan, EN Chini, SJ Gibbons, TA Kellogg, K Khazaie, M Kuro-O, JM Espindola, MP Singh, JG Tidball, M Wehling-He, G Farrugia, T Ordog Wnt-induced, TRP53-mediated Cell Cycle Arrest of Precursors Underlies Interstitial Cell of Cajal Depletion during Aging Cell Mol Gastroenterol Hepatol, 2020-08-07;0(0):. 2020-08-07 [PMID: 32771388] (Western Blot, Human, Mouse)	Western Blot	Human, Mouse
MM Mikedis, Y Fan, PK Nicholls, T Endo, EK Jackson, SA Cobb, DG de Rooij, DC Page DAZL mediates a broad translational program regulating expansion and differentiation of spermatogonial progenitors Elife, 2020-07-20;9(0):. 2020-07-20 [PMID: 32686646] (IHC-P, Mouse)	IHC-P	Mouse
Jessica M. Stringer, Amy Winship, Nadeen Zerafa, Matthew Wakefield, Karla Hutt Oocytes can efficiently repair DNA double-strand breaks to restore genetic integrity and protect offspring health Proceedings of the National Academy of Sciences 5/26/2020 [PMID: 32381741]
A Heinrich, SJ Potter, L Guo, N Ratner, T DeFalco Distinct Roles for Rac1 in Sertoli Cell Function during Testicular Development and Spermatogenesis Cell Rep, 2020-04-14;31(2):107513. 2020-04-14 [PMID: 32294451] (IF, Mouse)	IF	Mouse
Liu C, Ma Z, Cai Z et Al. Identification of primordial germ cell-like cells as liver metastasis initiating cells in mouse tumour models Cell Discov 3/24/2020 [PMID: 32218989]
Alcmène Chalazonitis, ZhiShan Li, Tuan D. Pham, Jason Chen, Meenakshi Rao, Päivi Lindholm et al. Cerebral dopamine neurotrophic factor is essential for enteric neuronal development, maintenance, and regulation of gastrointestinal transit Journal of Comparative Neurology 10/1/2020 [PMID: 32154930]
M Hou, J Han, G Li, MY Kwon, J Jiang, S Emani, ES Taglauer, JA Park, EB Choi, M Vodnala, YW Fong, SM Emani, IO Rosas, MA Perrella, X Liu Multipotency of mouse trophoblast stem cells Stem Cell Res Ther, 2020-02-13;11(1):55. 2020-02-13 [PMID: 32054514] (ICC, IHC, Human, Mouse)	ICC, IHC	Human, Mouse
Heather Tillman, Laura J. Janke, Amy Funk, Peter Vogel, Jerold E. Rehg Morphologic and Immunohistochemical Characterization of Spontaneous Lymphoma/Leukemia in NSG Mice Veterinary Pathology 1/1/2020 [PMID: 31736441]
So EY, Sun C, Wu KQ et al. Lipid phosphatase SHIP-1 regulates chondrocyte hypertrophy and skeletal development J. Cell. Physiol. 7/9/2019 [PMID: 31287165]
JMD Legrand, AL Chan, HM La, FJ Rossello, ML Änkö, FV Fuller-Pac, RM Hobbs DDX5 plays essential transcriptional and post-transcriptional roles in the maintenance and function of spermatogonia Nat Commun, 2019;10(1):2278. 2019 [PMID: 31123254] (IHC, Human)	IHC	Human
P Agarwal, S Isringhaus, H Li, AJ Paterson, J He, Á Gomariz, T Nagasawa, C Nombela-Ar, R Bhatia Mesenchymal Niche-Specific Expression of Cxcl12 Controls Quiescence of Treatment-Resistant Leukemia Stem Cells Cell Stem Cell, 2019-03-21;0(0):. 2019-03-21 [PMID: 30905620] (Neutralization, Mouse)	Neutralization	Mouse
H Kidoya, F Muramatsu, T Shimamura, W Jia, T Satoh, Y Hayashi, H Naito, Y Kunisaki, F Arai, M Seki, Y Suzuki, T Osawa, S Akira, N Takakura Regnase-1-mediated post-transcriptional regulation is essential for hematopoietic stem and progenitor cell homeostasis Nat Commun, 2019-03-06;10(1):1072. 2019-03-06 [PMID: 30842549] (IHC, Mouse)	IHC	Mouse
Takahiko Imai, Hiroki Tanaka, Yoko Hamazaki, Nagahiro Minato Rap1 signal modulators control the maintenance of hematopoietic progenitors in bone marrow and adult long‐term hematopoiesis Cancer Science 4/1/2019 [PMID: 30767320]
KI Korski, DA Kubli, BJ Wang, FG Khalafalla, MM Monsanto, F Firouzi, OH Echeagaray, T Kim, RM Adamson, WP Dembitsky, ÅB Gustafsson, MA Sussman Hypoxia Prevents Mitochondrial Dysfunction and Senescence in Human c-Kit+ Cardiac Progenitor Cells Stem Cells, 2019-01-30;0(0):. 1/30/2019 [PMID: 30629785]
G Liu, L Chen, Q Cai, H Wu, Z Chen, X Zhang, P Lu Streptozotocin?induced diabetic mice exhibit reduced experimental choroidal neovascularization but not corneal neovascularization Mol Med Rep, 2018-09-03;0(0):. 9/3/2018 [PMID: 30221697]
Hue M. La, Ai-Leen Chan, Julien M. D. Legrand, Fernando J. Rossello, Christina G. Gangemi, Antonella Papa et al. GILZ-dependent modulation of mTORC1 regulates spermatogonial maintenance Development 3/16/2018 [PMID: 30126904]
HM La, JA Mäkelä, AL Chan, FJ Rossello, CM Nefzger, JMD Legrand, M De Seram, JM Polo, RM Hobbs Identification of dynamic undifferentiated cell states within the male germline Nat Commun, 2018-07-19;9(1):2819. 7/19/2018 [PMID: 30026551]
Lina Vasiliauskaitė, Rebecca V. Berrens, Ivayla Ivanova, Claudia Carrieri, Wolf Reik, Anton J. Enright et al. Defective germline reprogramming rewires the spermatogonial transcriptome Nature Structural & Molecular Biology 5/1/2018 [PMID: 29728652]
Jiao Zhao, Jing An, Shi Liu Electroacupuncture at ST36 Increases Bone Marrow-Derived Interstitial Cells of Cajal via the SDF-1/CXCR4 and mSCF/Kit-ETV1 Pathways in the Stomach of Diabetic Mice Evidence-Based Complementary and Alternative Medicine 1/23/2018 [PMID: 29599809]
D Xie, Y Liao, B Wu, Y Chen, W Lin, D Lu, S Gao, S Zhu, C Peng, MH Jiang Cardiac Nestin+ Cells Derived from Early Stage of Dilated Cardiomyopathy Enhanced the Survival of the Doxorubicin-Injured Cardiac Muscle HL-1 Cells Int Heart J, 2018-01-01;59(1):180-189. 2018-01-01 [PMID: 29375111] (ICC, IHC, Mouse)	ICC, IHC	Mouse
ZF Hosseini, DA Nelson, N Moskwa, LM Sfakis, J Castracane, M Larsen FGF2-dependent mesenchyme and laminin-111 are niche factors in salivary gland organoids J. Cell. Sci., 2018-02-20;0(0):. 2/20/2018 [PMID: 29361536]
S Saeidi, F Shapouri, RU de Iongh, F Casagranda, JM Sutherland, PS Western, EA McLaughlin, M Familari, GR Hime Esrp1 is a marker of mouse fetal germ cells and differentially expressed during spermatogenesis PLoS ONE, 2018-01-11;13(1):e0190925. 2018-01-11 [PMID: 29324788] (IHC-P, Mouse)	IHC-P	Mouse
DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2017-11-13;15(1):39-46. 2017-11-13 [PMID: 29320487] (IHC, Mouse)	IHC	Mouse
Y Kawai, A Oda, Y Kanai, R Goitsuka Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis PLoS ONE, 2018-01-02;13(1):e0190702. 2018-01-02 [PMID: 29293683] (IHC-P, Mouse)	IHC-P	Mouse
JC Harrell, TM Shroka, BM Jacobsen Estrogen induces c-Kit and an aggressive phenotype in a model of invasive lobular breast cancer Oncogenesis, 2017-11-27;6(11):2. 2017-11-27 [PMID: 29176653] (Western Blot, Human)	Western Blot	Human
A Magadum, Y Ding, L He, T Kim, MD Vasudevara, Q Long, K Yang, N Wickramasi, HV Renikunta, N Dubois, G Weidinger, Q Yang, FB Engel Live cell screening platform identifies PPAR? as a regulator of cardiomyocyte proliferation and cardiac repair Cell Res., 2017-06-16;0(0):. 2017-06-16 [PMID: 28621328] (ICC, Mouse)	ICC	Mouse
Dexter A. Myrick, Michael A. Christopher, Alyssa M. Scott, Ashley K. Simon, Paul G. Donlin-Asp, William G. Kelly et al. KDM1A/LSD1 regulates the differentiation and maintenance of spermatogonia in mice PLOS ONE 5/12/2017 [PMID: 28498828]
C Carrieri, S Comazzetto, A Grover, M Morgan, A Buness, C Nerlov, D O'Carroll A transit-amplifying population underpins the efficient regenerative capacity of the testis J. Exp. Med., 2017-05-01;0(0):. 2017-05-01 [PMID: 28461596] (ICC, Mouse)	ICC	Mouse
MM Monsanto, KS White, T Kim, BJ Wang, K Fisher, K Ilves, FG Khalafalla, A Casillas, K Broughton, S Mohsin, WP Dembitsky, MA Sussman Concurrent Isolation of Three Distinct Cardiac Stem Cell Populations from a Single Human Heart Biopsy Circ. Res., 2017-04-26;0(0):. 2017-04-26 [PMID: 28446444] (ICC, Human)	ICC	Human
MY Lee, SE Ha, C Park, PJ Park, R Fuchs, L Wei, BG Jorgensen, D Redelman, SM Ward, KM Sanders, S Ro Transcriptome of interstitial cells of Cajal reveals unique and selective gene signatures PLoS ONE, 2017-04-20;12(4):e0176031. 2017-04-20 [PMID: 28426719] (IHC-Fr, Mouse)	IHC-Fr	Mouse
L Luo, J Tang, K Nishi, C Yan, PU Dinh, J Cores, T Kudo, J Zhang, TS Li, K Cheng Fabrication of Synthetic Mesenchymal Stem Cells for the Treatment of Acute Myocardial Infarction in Mice Circ. Res, 2017-03-15;0(0):. 2017-03-15 [PMID: 28298296] (IHC, Mouse)	IHC	Mouse
AL Feng, YY Xiang, L Gui, G Kaltsidis, Q Feng, WY Lu Paracrine GABA and insulin regulate pancreatic alpha cell proliferation in a mouse model of type 1 diabetes Diabetologia, 2017-03-09;0(0):. 2017-03-09 [PMID: 28280900] (IHC, Mouse)	IHC	Mouse
Kellie S. Agrimson, Jennifer Onken, Debra Mitchell, Traci B. Topping, Hélio Chiarini-Garcia, Cathryn A. Hogarth et al. Characterizing the Spermatogonial Response to Retinoic Acid During the Onset of Spermatogenesis and Following Synchronization in the Neonatal Mouse Testis1 Biology of Reproduction 10/13/2016 [PMID: 27488029]
Radiation Exposure Decreases the Quantity and Quality of Cardiac Stem Cells in Mice PLoS ONE, 2016-05-19;11(5):e0152179. 2016-05-19 [PMID: 27195709] (IHC, Mouse)	IHC	Mouse
Oraly Sanchez-Ferras, Guillaume Bernas, Omar Farnos, Aboubacrine M. Touré, Ouliana Souchkova, Nicolas Pilon A direct role for murine Cdx proteins in the trunk neural crest-gene regulatory network Development 4/15/2016 [PMID: 26952979]
Liu Q, Yang R, Huang X, Zhang H, He L, Zhang L, Tian X, Nie Y, Hu S, Yan Y, Zhang L, Qiao Z, Wang Q, Lui K, Zhou B Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes. Cell Res, 2015-12-04;26(1):119-30. 2015-12-04 [PMID: 26634606] (ICC, Mouse)	ICC	Mouse
Nishat Sultana, Lu Zhang, Jianyun Yan, Jiqiu Chen, Weibin Cai, Shegufta Razzaque et al. Resident c-kit(+) cells in the heart are not cardiac stem cells Nature Communications 10/30/2015 [PMID: 26515110]
Tanaka Y, Inoue-Yokoo T, Kulkeaw K, Yanagi-Mizuochi C, Shirasawa S, Nakanishi Y, Sugiyama D Embryonic Hematopoietic Progenitor Cells Reside in Muscle before Bone Marrow Hematopoiesis. PLoS ONE, 2015-09-21;10(9):e0138621. 2015-09-21 [PMID: 26389592] (IHC, Mouse)	IHC	Mouse
Terry L. Powley, Cherie N. Hudson, Jennifer L. McAdams, Elizabeth A. Baronowsky, Robert J. Phillips Vagal Intramuscular Arrays: The Specialized Mechanoreceptor Arbors That Innervate the Smooth Muscle Layers of the Stomach Examined in the Rat Journal of Comparative Neurology 3/1/2016 [PMID: 26355387]
Quijada P, Hariharan N, Cubillo J, Bala K, Emathinger J, Wang B, Ormachea L, Bers D, Sussman M, Poizat C Nuclear Calcium/Calmodulin-dependent Protein Kinase II Signaling Enhances Cardiac Progenitor Cell Survival and Cardiac Lineage Commitment. J Biol Chem, 2015-08-31;290(42):25411-26. 2015-08-31 [PMID: 26324717] (IHC-P, Mouse)	IHC-P	Mouse
Iglesias J, Cairney C, Ferrier R, McDonald L, Soady K, Kendrick H, Pringle M, Morgan R, Martin F, Smalley M, Blyth K, Stein T Annexin A8 identifies a subpopulation of transiently quiescent c-kit positive luminal progenitor cells of the ductal mammary epithelium. PLoS ONE, 2015-03-24;10(3):e0119718. 2015-03-24 [PMID: 25803307] (IHC-P, Mouse)	IHC-P	Mouse
Shiu J, Piazuelo M, Ding H, Czinn S, Drakes M, Banerjee A, Basappa N, Kobayashi K, Fricke W, Blanchard T Gastric LTi cells promote lymphoid follicle formation but are limited by IRAK-M and do not alter microbial growth. Mucosal Immunol, 2015-01-21;8(5):1047-59. 2015-01-21 [PMID: 25603827] (IHC-P, Mouse)	IHC-P	Mouse
Kay-Dietrich Wagner, Julien Cherfils-Vicini, Naoki Hosen, Peter Hohenstein, Eric Gilson, Nicholas D. Hastie et al. The Wilms’ tumour suppressor Wt1 is a major regulator of tumour angiogenesis and progression Nature Communications 12/16/2014 [PMID: 25510679]
Kohtarou Konno, Hiromi Takahashi-Iwanaga, Motokazu Uchigashima, Kyoko Miyasaka, Akihiro Funakoshi, Masahiko Watanabe et al. Cellular and subcellular localization of cholecystokinin (CCK)-1 receptors in the pancreas, gallbladder, and stomach of mice Histochemistry and Cell Biology 3/1/2015 [PMID: 25249350]
Smith A, Lewis F, Aquila I, Waring C, Nocera A, Agosti V, Nadal-Ginard B, Torella D, Ellison G Isolation and characterization of resident endogenous c-Kit+ cardiac stem cells from the adult mouse and rat heart. Nat Protoc, 2014-06-19;9(7):1662-81. 2014-06-19 [PMID: 24945383] (ICC, Mouse)	ICC	Mouse
Hiromichi Shimizu, Ryuichi Okamoto, Go Ito, Satoru Fujii, Toru Nakata, Kohei Suzuki et al. Distinct expression patterns of Notch ligands, Dll1 and Dll4, in normal and inflamed mice intestine PeerJ 5/1/2014 [PMID: 24860699]
van Berlo J, Kanisicak O, Maillet M, Vagnozzi R, Karch J, Lin S, Middleton R, Marban E, Molkentin J c-kit+ cells minimally contribute cardiomyocytes to the heart. Nature, 2014-05-07;0(0):. 2014-05-07 [PMID: 24805242] (Western Blot, Mouse)	Western Blot	Mouse
Toko , Haruhiro, Hariharan , Nirmala, Konstandin , Mathias, Ormachea , Lucia, McGregor , Michael, Gude , Natalie, Sundararaman , Balaji, Joyo , Eri, Joyo , Anya Y, Collins , Brett, Din , Shabana, Mohsin , Sadia, Uchida , Takafumi, Sussman , Mark A Differential regulation of cellular senescence and differentiation by prolyl isomerase Pin1 in cardiac progenitor cells. J Biol Chem, 2013-12-27;289(9):5348-56. 2013-12-27 [PMID: 24375406] (IHC, Mouse)	IHC	Mouse
Michael McGregor, Nirmala Hariharan, Anya Y Joyo, Robert L Margolis, Mark A Sussman CENP-A is essential for cardiac progenitor cell proliferation Cell Cycle 3/1/2014 [PMID: 24362315]
Ye L, Zhang P, Duval S, Su L, Xiong Q, Zhang J Thymosin beta4 increases the potency of transplanted mesenchymal stem cells for myocardial repair. Circulation, 2013-09-10;128(11):S32-41. 2013-09-10 [PMID: 24030419] (IHC-P, Porcine)	IHC-P	Porcine
Fang , Shentong, Wei , Jing, Pentinmikko , Nalle, Leinonen , Hannele, Salven , Petri Generation of Functional Blood Vessels from a Single c-kit+ Adult Vascular Endothelial Stem Cell. PLoS Biol, 2012-10-16;10(10):e1001407. 2012-10-16 [PMID: 23091420] (IHC, Mouse)	IHC	Mouse
Terry L. Powley, Jared M. Gilbert, Elizabeth A. Baronowsky, Cherie N. Billingsley, Felecia N. Martin, Robert J. Phillips Vagal sensory innervation of the gastric sling muscle and antral wall: implications for gastro-esophageal reflux disease? Neurogastroenterology & Motility 10/1/2012 [PMID: 22925069]
Nakamura-Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, Nagasawa T, Suda T, Kubota Y Extracellular matrix protein tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration. Blood, 2012-05-02;119(23):5429-37. 2012-05-02 [PMID: 22553313] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Moon TC, Yoshimura T, Parsons T, Befus AD Microenvironmental regulation of inducible nitric oxide synthase expression and nitric oxide production in mouse bone marrow-derived mast cells. J. Leukoc. Biol., 2012-01-18;91(4):581-90. 2012-01-18 [PMID: 22262798] (ICC, Mouse)	ICC	Mouse
Koh BH, Roy R, Hollywood MAet al. PDGFRa cells in mouse urinary bladder: A new class of interstitial cells. J Cell Mol Med 12/12/2011 [PMID: 22151424]
Miyamoto K, Yoshida S, Kawasumi M Osteoclasts are dispensable for hematopoietic stem cell maintenance and mobilization. J. Exp. Med., 2011-10-17;208(11):2175-81. 2011-10-17 [PMID: 22006978] (IHC, Mouse)	IHC	Mouse
Gaoqin Liu, Peirong Lu, Longbiao Li, Hui Jin, Xuefei He, Naofumi Mukaida et al. Critical role of SDF-1 alpha -induced progenitor cell recruitment and macrophage VEGF production in the experimental corneal neovascularization Mol Vis 8/10/2011 [PMID: 21850188]
Yuichi Tamura, Keisuke Matsumura, Motoaki Sano, Hidenori Tabata, Kensuke Kimura, Masaki Ieda et al. Neural Crest–Derived Stem Cells Migrate and Differentiate Into Cardiomyocytes After Myocardial Infarction Arteriosclerosis, Thrombosis, and Vascular Biology 3/1/2011 [PMID: 21212399]
Jenna M. Tabor-Godwin, Chelsea M. Ruller, Nolan Bagalso, Naili An, Robb R. Pagarigan, Stephanie Harkins et al. A Novel Population of Myeloid Cells Responding to Coxsackievirus Infection Assists in the Dissemination of Virus within the Neonatal CNS Journal of Neuroscience 6/23/2010 [PMID: 20573913]
Fifadara NH, Aye CC, Raghuwanshi SK, Richardson RM, Ono SJ CCR1 expression and signal transduction by murine BMMC results in secretion of TNF-alpha, TGFbeta-1 and IL-6. Int. Immunol., 2009-07-10;21(8):991-1001. 2009-07-10 [PMID: 19592420] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
Shin JS, Shelburne CP, Jin C, LeFurgey EA, Abraham SN Harboring of particulate allergens within secretory compartments by mast cells following IgE/FcepsilonRI-lipid raft-mediated phagocytosis. J. Immunol., 2006-11-01;177(9):5791-800. 2006-11-01 [PMID: 17056503] (ICC, Mouse)	ICC	Mouse
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Reviews for CD117/c-kit Antibody (AF1356) (4) 4.34

Average Rating: 4.3

(Based on 4 reviews)

We have 4 reviews tested in 1 species: Mouse.

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03/23/2018

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Application	Western Blot
Sample Tested	mouse es cells
Species	Mouse
Lot	IEO0217011

reviewed by:
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Mouse

01/05/2015

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Application	Western Blot
Sample Tested	See PMID 24375406
Species	Mouse

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01/05/2015

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Application	Immunofluorescence
Sample Tested	See PMID 22262798
Species	Mouse

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Mouse

01/05/2015

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Application	Immunocytochemistry
Sample Tested	See PMID 24375406
Species	Mouse

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Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene ... Read full blog post.

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SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog #AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog #F0107).

Goat IgG Isotype Control [Unconjugated]
AB-108-C

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.

TNF-alpha [Unconjugated]
210-TA-005

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4.3

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	Verified Customer 03/23/2018
Application:	WB
Species:	Mouse

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	Verified Customer 01/05/2015
Application:	WB
Species:	Mouse

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	Verified Customer 01/05/2015
Application:	IF
Species:	Mouse

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Bioinformatics

Gene Symbol	KIT
Uniprot	P05532()

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

CD117/c-kit Antibody [Unconjugated]

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Publications for CD117/c-kit Antibody (AF1356)(110)

Reviews for CD117/c-kit Antibody (AF1356) (4) 4.34

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