![Western Blot: Calmodulin Antibody (2D1) [NB120-2860] - Purified Calmodulin using NB120-2860 and NB120-5494.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Western-Blot-NB120-2860-img0028.jpg "Western Blot: Calmodulin Antibody (2D1) [NB120-2860] - Purified Calmodulin using NB120-2860 and NB120-5494.")
| Reactivity | Hu, Mu, Rt, Ba, Bv, ChSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clone | 2D1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Calmodulin purified from Dictyostelium discoideum. |
| Specificity | This detects calmodulin. It does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK). |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CALM1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Detects an approx. 17 kDa protein representing calmodulin from Dictyostelium cell lysate. Addition of EDTA to buffers completely inhibits antibody staining. IHC: Staining of calmodulin in Dictyostelium cells with this antibody results in staining of the contractile vacuoles. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Calmodulin Antibody (NB120-2860)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Staining of rat brain.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NB120-2860-img0008.jpg "Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Staining of rat brain.")
![Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) [NB120-2860] - Normal biopsies of deparaffinized Rat testis tissue.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunohistochemistry-Paraffin-NB120-2860-img0013.jpg "Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) [NB120-2860] - Normal biopsies of deparaffinized Rat testis tissue.")
![Flow Cytometry: Calmodulin Antibody (2D1) [NB120-2860] - Analysis of Calmodulin in MCF-7 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Flow-Cytometry-NB120-2860-img0020.jpg "Flow Cytometry: Calmodulin Antibody (2D1) [NB120-2860] - Analysis of Calmodulin in MCF-7 cells compared to an isotype control (blue).")
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.A2058 melanoma cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NB120-2860-img0015.jpg "Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.A2058 melanoma cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. C6 glioma cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NB120-2860-img0016.jpg "Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. C6 glioma cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. HeLa Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunocytochemistry-Immunofluorescence-NB120-2860-img0017.jpg "Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. HeLa Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) [NB120-2860] - Normal biopsies of deparaffinized Rat cerebellum tissue.](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Immunohistochemistry-Paraffin-NB120-2860-img0012.jpg "Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) [NB120-2860] - Normal biopsies of deparaffinized Rat cerebellum tissue.")
![Flow Cytometry: Calmodulin Antibody (2D1) [NB120-2860] - Analysis of Calmodulin in C6 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Flow-Cytometry-NB120-2860-img0018.jpg "Flow Cytometry: Calmodulin Antibody (2D1) [NB120-2860] - Analysis of Calmodulin in C6 cells compared to an isotype control (blue).")
![Flow Cytometry: Calmodulin Antibody (2D1) [NB120-2860] - Analysis of Calmodulin in PC12 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/Calmodulin-Antibody-2D1-Flow-Cytometry-NB120-2860-img0019.jpg "Flow Cytometry: Calmodulin Antibody (2D1) [NB120-2860] - Analysis of Calmodulin in PC12 cells compared to an isotype control (blue).")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")