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APBB3 Antibody (OTI5G3)

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Western Blot: APBB3 Antibody (5G3) [NBP2-01367] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APBB3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: APBB3 Antibody (5G3) [NBP2-01367] Staining of paraffin-embedded Human prostate tissue using anti-APBB3 mouse monoclonal antibody.
Flow Cytometry: APBB3 Antibody (5G3) [NBP2-01367] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-APBB3 antibody, and then analyzed ...read more
Immunohistochemistry-Paraffin: APBB3 Antibody (5G3) [NBP2-01367] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-APBB3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: APBB3 Antibody (5G3) [NBP2-01367] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-APBB3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: APBB3 Antibody (5G3) [NBP2-01367] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-APBB3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: APBB3 Antibody (5G3) [NBP2-01367] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-APBB3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: APBB3 Antibody (5G3) [NBP2-01367] - Staining of paraffin-embedded Human colon tissue using anti-APBB3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: APBB3 Antibody (5G3) [NBP2-01367] - Staining of paraffin-embedded Human endometrium tissue using anti-APBB3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: APBB3 Antibody (5G3) [NBP2-01367] - Staining of paraffin-embedded Human pancreas tissue using anti-APBB3 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI5G3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.39 mg/ml

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APBB3 Antibody (OTI5G3) Summary

Immunogen
Full length human recombinan protein of human APBB3(NP_573419) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
APBB3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500
Theoretical MW
52.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.39 mg/ml
Purity
Immunogen affinity purified

Alternate Names for APBB3 Antibody (OTI5G3)

  • amyloid beta (A4) precursor protein-binding, family B, member 3
  • amyloid precursor interacting protein
  • Fe65L2
  • FE65L2amyloid beta A4 precursor protein-binding family B member 3
  • FE65-like protein 2
  • MGC150555
  • MGC87674
  • Protein Fe65-like 2
  • SRA

Background

The protein encoded by this gene is a member of the APBB protein family. It is found in the cytoplasm and binds to the intracellular domain of the Alzheimer's disease beta-amyloid precursor protein (APP) as well as to other APP-like proteins. It is thought that the protein encoded by this gene may modulate the internalization of APP. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol APBB3
Entrez