Novus Biologicals is one of the leading antibody suppliers of high affinity human epitope tag reagents, such as the HA tag antibody. Widely used in Western blot, ELISA and Immunohistochemistry assays, they offer a useful and reliable method for detecting, analyzing and purifying proteins from a wide range of species.
Short, immunoreactive epitope tags are highly useful reagents. They are small enough to avoid interfering with the protein under test, yet have a high degree of immunoreactivity, due to the fact they are generally derived from viral genes. The HA tag, for example, is derived from amino acids 98-106 of the human influenza haemagglutinin (HA) protein, an antigenic glycoprotein found on the surface of the human influenza virus.
Developed around 20 years ago, the HA tag can be expressed from a wide range of engineered recombinant proteins, and is widely used as a general epitope tag for expression vectors. The HA tag antibody recognizes over-expressed HA-tagged proteins in a wide range of cells, including transfected mammalian cells. For easy immunochemical analysis and visualization, it is usually engineered onto the N (amino) or C (carboxy) terminus of the protein being studied.
The HA tag antibody has proven invaluable in human disease research, being extensively used in both biochemistry and cell biology to track and isolate proteins, and precipitate them with other proteins in the pathway. They have proven invaluable in the complex field of apoptosis. However, in 2007 H. Zhang, et al. reported HA tag cleavage and loss of immunoreactivity during apoptosis. Fortunately, Novus also offers plenty of alternatives, such as c-Myc and V-5 epitope tags.
Novus Biologicals offers many HA tag reagents for your research needs including:
