![Western Blot: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Aldo-keto Reductase 1C4/AKR1C4 monoclonal antibody (M01), clone 2C11 Western Blot analysis of Aldo-keto Reductase 1C4/AKR1C4 expression in MCF-7 [L046V1].](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C4-AKR1C4-Antibody-2C11-Western-Blot-H00001109-M01-img0018.jpg "Western Blot: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Aldo-keto Reductase 1C4/AKR1C4 monoclonal antibody (M01), clone 2C11 Western Blot analysis of Aldo-keto Reductase 1C4/AKR1C4 expression in MCF-7 [L046V1].")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 2C11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | AKR1C4 (AAH20744, 1 a.a. ~ 323 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDPKYQRVELNDGHFMPVLGFGTYAPPEVPRNRAVEVTKLAIEAGFRHIDSAYLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWCTFFQPQMVQPALESSLKKLQLDYVDLYLLHFPMALKPGETPLPKDENGKVIFDTVDLSATWEVMEKCKDAGLAKSIGVSNFNYRQLEMILNKPGLKYKPVCNQVECHPYLNQSKLLDFCKSKDIVLVAHSALGTQRHKLWVDPNSPVLLEDPVLCALAKKHKRTPALIALRYQLQRGVVVLAKSYNEQRIRENIQVFEFQLTSEDMKVLDGLNRNYRYVVMDFLMDHPDYPFSDEY |
| Specificity | AKR1C4 - aldo-keto reductase family 1, member C4 (chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | AKR1C4 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Aldo-keto Reductase 1C4/AKR1C4 Antibody (H00001109-M01)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Analysis of monoclonal antibody to AKR1C4 on HepG2 cell. Antibody concentration 15 ug/ml.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C4-AKR1C4-Antibody-2C11-Immunocytochemistry-Immunofluorescence-H00001109-M01-img0011.jpg "Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Analysis of monoclonal antibody to AKR1C4 on HepG2 cell. Antibody concentration 15 ug/ml.")
![Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Analysis of monoclonal antibody to AKR1C4 on formalin-fixed paraffin-embedded human liver tissue. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C4-AKR1C4-Antibody-2C11-Immunohistochemistry-Paraffin-H00001109-M01-img0013.jpg "Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Analysis of monoclonal antibody to AKR1C4 on formalin-fixed paraffin-embedded human liver tissue. Antibody concentration 3 ug/ml.")
![Western Blot: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Analysis of AKR1C4 expression in transfected 293T cell line by AKR1C4 monoclonal antibody (M01), clone 2C11.Lane 1: AKR1C4 transfected lysate(37.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C4-AKR1C4-Antibody-2C11-Western-Blot-H00001109-M01-img0016.jpg "Western Blot: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Analysis of AKR1C4 expression in transfected 293T cell line by AKR1C4 monoclonal antibody (M01), clone 2C11.Lane 1: AKR1C4 transfected lysate(37.2 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Aldo-keto reductases contribute to melphalan resistance in MM cells. Western analysis confirmed up-regulation of AKR1C2 and C3, but not C4. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0119857), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C4-AKR1C4-Antibody-2C11-Western-Blot-H00001109-M01-img0017.jpg "Western Blot: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Aldo-keto reductases contribute to melphalan resistance in MM cells. Western analysis confirmed up-regulation of AKR1C2 and C3, but not C4. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0119857), licensed under a CC-BY license.")
![Sandwich ELISA: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Detection limit for recombinant GST tagged AKR1C4 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C4-AKR1C4-Antibody-2C11-Sandwich-ELISA-H00001109-M01-img0014.jpg "Sandwich ELISA: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Detection limit for recombinant GST tagged AKR1C4 is 3 ng/ml as a capture antibody.")
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![Western Blot Aldo-keto Reductase 1C1/AKR1C1 Antibody (859026) [Unconjugated]](https://images.novusbio.com/images2/Aldoketo_Reductase_1C1_MAB6529_Western_Blot_13068.jpg)
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")