I would be very grateful if you could let me know a recommended dilution for the VEGF antibody antibody NB100-664

The recommended dilution for IHC is 1:20-1:50 in an ABC method. If instead you are testing on cell culture, I would recommend a starting optimization point of 1:10 -1:2000.

I would like to know the aproximate dilution of the VEGF Antibody (NB100-664) to use it in a Western blot assay.

In regards to your inquiry about our VEGF antibody (NB100-664), I am showing from the lab data that they have tested dilutions in the range of 1:500-1:1000 for this antibody. The optimal dilution should depend on your samples and the level of expression of VEGF, but a good starting point would be 1:1000 and then optimize from there.

What is the minimum amount of protein necessary for the detection of VEGF by Western blot using VEGF Antibody (NB100-664)?

I would suggest at minimum 10ug of protein per well but if you have enough sample you might want to run multiple wells with increasing amounts of protein to optimize the conditions in your hands. You could start with 5ug and do a couple of wells with 10ug, 20ug and 40ug. Also try using different dilutions of primary, start with 1:500-1:1000 and work your way up or down depending on what your blot looks like.

I am interested in buying an antibody to VEGF to detect VEGF in human umbilical vein endothelial cells for western blotting and was wondering if you might have any recommendations? I was thinking about NB100-664. Do you have a recommended amount to use for western blotting? Could I ask you what concentration the antibody is at since it says 0.1mg so what total volume do you send? Also is this the most commonly purchase one for western blotting do you know?

We do not have a recommended dilution noted for this specific antibody, but our general recommendation for Western blot is 1:1000-1:2000.This is a good starting point, but we always recommend optimization by the end user, as the optimal dilution is to some extent dependent on the particular sample/assay. The concentration for this product is stated as 1 mg/ml, so 0.1 mg would be supplied in 0.1 ml.

I am looking for a VEGF-A antibody for the IHC staining of mouse colon. Do you have any publications or application references for this product?

NB100-664 is one of our best sellers with great customer feedback and citations in at least 18 peer reviewed publications from highly esteemed researchers. As far as mouse tissues are concerned, I would recommend that you go through the following paper: Milanesi A, et al. Beta-Cell regeneration mediated by human bone marrow mesenchymal stem cells. PLoS One. 2012;7(8):e42177. doi: 10.1371/journal.pone.0042177. Epub 2012 Aug 7. PMID:22879915 .

Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?

I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

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VEGF Antibody (VG1) - BSA Free

Name: VEGF Antibody (VG1) - BSA Free
Brand: Novus Biologicals
SKU: NB100-664
Rating: 5 (1 reviews)

(89 Publications)

(1 Review)

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Analysis of VEGF in human kidney protein using NB100-664.

U87 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB100-664 at 1 ug/ml overnight at 4C and detected with an ...read more

Immunohistochemistry: VEGF Antibody (VG1) - BSA Free [NB100-664] - HPG induces less VEGF production, less myofibroblast differentiation and lower macrophage activation. The expression of VEGF, alpha-SMA and MAC387 in ...read more

An intracellular stain was performed on U-937 cells with NB100-664 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an ...read more

After transfection with 50 nmol/L miR-1956 mimic for 3 days, the levels of secreted and cellular VEGF were evaluated using western blotting. Representative images of four independent experiments are shown. Data are ...read more

HUVEC and 293T cells transfected with a plasmid expressing human VEGF165 at 1:1000. Image provided by verified customer review.

U-87 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB100-664 at 2 ug/ml overnight at 4C and detected with an ...read more

FFPE human angiosarcoma tissue section using VEGF antibody (clone VG1). The endothelial cells of the blood vessels and most of the cancer cells showed strong positivity for VEGF protein.

Analysis of PerCP conjugate of NB100-664. An intracellular stain was performed on HUVEC cells with VEGF (VG1) antibody NB100-664PCP (blue) and a matched isotype control NBP2-27287PCP (orange). Cells were fixed with 4% ...read more

An intracellular stain was performed on U-937 cells with NB100-664F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an ...read more

HPG induces less VEGF production, less myofibroblast differentiation and lower macrophage activation. The expression of VEGF, alpha-SMA and MAC387 in the peritoneal tissue sections was examined using a routine ...read more

Increased p62 expression is prevented in PINK1 knock‐down cells. (a) SH‐SY5Y cells expressing scrambled shRNA or PINK1 shRNA (117‐2, 118‐3, and 118‐4) were treated with 10 μM carbonyl cyanide ...read more

Aryl hydrocarbon receptor interacting protein (AIP) immunostaining.A and B—Examples of low AIP expression; C and D: Examples of high AIP expression; E—Normal human pituitary staining with omitting primary antibody ...read more

Involvement of OGT in histone methylase MLL/COMPASS-like complex-mediated Nrf2 expression in SNUC5/5-FUR cellsA. OGT and O-GlcNAc expression was determined by Western blotting. *Significantly different from SNUC5 cells ...read more

SC-78, a derivative of regorafenib, exhibited more potent anti-migratory effects than regorafenib.(A) Chemical structure of SC-78. (B) The protein levels of p-VEGFR2, VEGFR2, p-PDGFR, and PDGFR in EAhy926 treated with ...read more

Characterization and internalization of M2-Exos. (A) TEM image of M2-Exos. Scale bar: 200 nm. (B) DLS measurement of M2-Exos size. (C) Western blotting assay of exosomal markers in THP-1-M2 cellular lysate and M2-Exos ...read more

Product Details

Summary

Reactivity	Hu, Mu, Rt, Po, CaSpecies Glossary
Applications	WB, Simple Western, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone	VG1
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Format	BSA Free
Concentration	1 mg/ml

Order Details

View Available Conjugates

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB100-664AF350

Alexa Fluor 405 NB100-664AF405

Alexa Fluor 488 NB100-664AF488

Alexa Fluor 532 NB100-664AF532

Alexa Fluor 594 NB100-664AF594

Alexa Fluor 647 NB100-664AF647

Alexa Fluor 700 NB100-664AF700

Alexa Fluor 750 NB100-664AF750

Allophycocyanin NB100-664APC

Biotin NB100-664B

DyLight 350 NB100-664UV

DyLight 405 NB100-664V

DyLight 488 NB100-664G

DyLight 550 NB100-664R

DyLight 594 NB100-664DL594

DyLight 650 NB100-664C

DyLight 680 NB100-664FR

DyLight 755 NB100-664IR

FITC NB100-664F

HRP NB100-664H

Janelia Fluor 549 NB100-664JF549

Janelia Fluor 646 NB100-664JF646

PE NB100-664PE

PerCP NB100-664PCP

mFluor Violet 450 SE NB100-664MFV450

mFluor Violet 500 SE NB100-664MFV500

mFluor Violet 610 SE NB100-664MFV610

View Available Formulations

Catalog# & Formulation	Size	Price
BSA Free NB100-664SS

Azide and BSA Free NBP2-81007

100% Guarantee on every product

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-664AF350
Alexa Fluor 405	NB100-664AF405
Alexa Fluor 488	NB100-664AF488
Alexa Fluor 532	NB100-664AF532
Alexa Fluor 594	NB100-664AF594
Alexa Fluor 647	NB100-664AF647
Alexa Fluor 700	NB100-664AF700
Alexa Fluor 750	NB100-664AF750
Allophycocyanin	NB100-664APC
Biotin	NB100-664B
DyLight 350	NB100-664UV
DyLight 405	NB100-664V
DyLight 488	NB100-664G
DyLight 550	NB100-664R
DyLight 594	NB100-664DL594
DyLight 650	NB100-664C
DyLight 680	NB100-664FR
DyLight 755	NB100-664IR
FITC	NB100-664F
HRP	NB100-664H
Janelia Fluor 549	NB100-664JF549
Janelia Fluor 646	NB100-664JF646
PE	NB100-664PE
PerCP	NB100-664PCP
mFluor Violet 450 SE	NB100-664MFV450
mFluor Violet 500 SE	NB100-664MFV500
mFluor Violet 610 SE	NB100-664MFV610

VEGF Antibody (VG1) - BSA Free Summary

Immunogen	Recombinant VEGF 189 protein.
Specificity	This VEGF Antibody (VG1) detects the 189, 165 and 121 isoforms of VEGF
Isotype	IgG1 Kappa
Clonality	Monoclonal
Host	Mouse
Gene	VEGFA
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

CyTOF-ready
ELISA
Flow Cytometry
Immunocytochemistry/ Immunofluorescence 1:10-1:500
Immunohistochemistry 1:20-1:100
Immunohistochemistry-Frozen 1:20-1:100
Immunohistochemistry-Paraffin 1:20-1:100
Simple Western
Western Blot 1-2 ug/ml

Application Notes

In IHC a dilution of 1:20-1:50 was used in an ABC method. However, depending on the staining conditions employed, we suggest that the final dilution should be determined by the user. We suggest an incubation period of 30-60 minutes at room temperature. High temperature treatment of formalin-fixed tissue sections using 1mM EDTA, pH 8.0 must be performed prior to the immunostaining. This antibody is CyTOF ready.
Simple Western reported by an internal validation. Separated by Size-All; matrix was 12-230 kDa.

Control

Human Kidney Whole Tissue Lysate (Adult Whole Normal)
Human Artery Whole Tissue Lysate (Adult Whole Normal)

Reviewed Applications

Read 1 Review rated 5

using
NB100-664 in the following applications:

Western Blot

Publications

Read Publications using
NB100-664 in the following applications:

Chemotaxis
1 publication
ELISA
2 publications
FLOW
1 publication
ICC
1 publication
ICC/IF
5 publications
IF/IHC
10 publications
IHC
4 publications
IHC-Fr
2 publications
IHC-P
8 publications
SPR
1 publication
WB
24 publications

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34423682). Use in Porcine reported in scientific literature (PMID:32132871).

Packaging, Storage & Formulations

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.02% Sodium Azide
Concentration	1 mg/ml
Purity	Protein G purified

Alternate Names for VEGF Antibody (VG1) - BSA Free

MVCD1
VAS
vascular endothelial growth factor A
Vascular permeability factor
Vasculotropin
VEGF
VEGFA
VEGF-A
VEGFMGC70609
VPF
VPFvascular endothelial growth factor

Background

Vascular endothelial growth factor (VEGF), also called VEGF-A and vascular permeability factor (VPF), is a secreted homodimeric glycoprotein belonging to the VEGF family with a role in stimulating angiogenesis and vasculogenesis (1,2). More specifically, VEGF-A secretion from most cell types contributes to promoting endothelial cell proliferation and migration, inhibiting apoptosis, increasing vascular permeability, and wound healing (1). The VEGF family consists of several members including VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, VEGF-F, and placenta growth factor (PLGF) (1-4). As a result of alternative splicing of the eight exon VEGFA gene, there are several VEGF-A protein isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length, with VEGF121 and VEGF165 being the two most expressed isoforms (1,5). Full length VEGF-A monomer has a 26 aa signal sequence plus a 206 aa (VEGF206) sequence, with a theoretic molecular weight (MW) of 27 kDa, containing VEGF receptor 1 (VEGFR1) and VEGR2 binding sites and heparin-binding domains (1-3,5,6). VEGF121 lacks heparin affinity and binds the receptor tyrosine kinases (RTKs) VEGFR1 and VEGFR2, whereas VEGF165 has moderate affinity for heparin and, in addition to being a ligand for VEGFR1 and VEGFR2, can also bind the co-receptors neuropilin 1 (NRP1) and NRP2 (1,5). Hypoxia and hypoxia-related genes such as HIF-1, EGF, and PDGF are major regulators angiogenesis and VEGF expression (1,3). VEGF signaling initiated by ligand binding to its receptors results in activation of different pathways including PI3K and MAPK and ultimately guides endothelial cell proliferation, migration, and survival (1,3). While VEGF plays an important role in promoting normal angiogenesis and blood vessel formation, its expression is often upregulated in tumors and other angiogenesis-related pathologies like osteroarthritis (OA) (1-5,7). Given its function, VEGF and its receptors have become a therapeutic target for treating cancer and blocking angiogenesis (4,5,7). A recombinant humanized monoclonal anti-VEGFA antibody called bevacizumab (Avastin) was first approved by the FDA in 2004 for the treatment of a number of cancers (1-3,5). Cancer patients may experience resistance to anti-VEGF antibodies and, as such, clinical studies are exploring combination treatment options with chemotherapies and immune-checkpoint inhibitors (3,5).

References

1. Melincovici CS, Bosca AB, susman S, et al. Vascular endothelial growth factor (VEGF) - key factor in normal and pathological angiogenesis. Rom J Morphol Embryol. 2018;59(2):455-467.

2. Shaik F, Cuthbert GA, Homer-Vanniasinkam S, Muench SP, Ponnambalam S, Harrison MA. Structural Basis for Vascular Endothelial Growth Factor Receptor Activation and Implications for Disease Therapy. Biomolecules. 2020;10(12):1673. https://doi.org/10.3390/biom10121673

3. Apte RS, Chen DS, Ferrara N. VEGF in Signaling and Disease: Beyond Discovery and Development. Cell. 2019;176(6):1248-1264. https://doi.org/10.1016/j.cell.2019.01.021

4. Matsumoto K, Ema M. Roles of VEGF-A signalling in development, regeneration, and tumours. J Biochem. 2014;156(1):1-10. https://doi.org/10.1093/jb/mvu031

5. Itatani Y, Kawada K, Yamamoto T, Sakai Y. Resistance to Anti-Angiogenic Therapy in Cancer-Alterations to Anti-VEGF Pathway. Int J Mol Sci. 2018;19(4):1232. Published 2018 Apr 18. doi:10.3390/ijms19041232

6. Uniprot (P15692)

7. Hamilton JL, Nagao M, Levine BR, Chen D, Olsen BR, Im HJ. Targeting VEGF and Its Receptors for the Treatment of Osteoarthritis and Associated Pain. J Bone Miner Res. 2016;31(5):911-924. https://doi.org/10.1002/jbmr.2828

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-664

Species: Hu, Mu, Rt, Po, Ca

Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, CyTOF-ready

Publications for VEGF Antibody (NB100-664)(89)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 11 applications: Chemotaxis, ELISA, FLOW, ICC, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, SPR, WB.

Showing Publications 1 - 10 of 89. Show All 89 Publications.

Publications using NB100-664	Applications	Species
Goudreau AD, Tanara L, Tzaneva V, Adamo KB Examining the Effects of Gestational Physical Activity and Hofbauer Cell Polarization on Angiogenic Factors International journal of environmental research and public health 2023-07-04 [PMID: 37444145] (ICC/IF, Human) Details: 1:100 ICC/IF dilution	ICC/IF	Human
Goudreau AD, Everest C, Tanara L et al. Characterization of Hofbauer cell polarization and VEGF localization in human term placenta from active and inactive pregnant individuals Physiological reports 2023-06-01 [PMID: 37269190] (IHC-Fr, Human)	IHC-Fr	Human
Lee Y, Lee J, Han H et al. Gingival biotype modification with collagen matrix or autogenous subepithelial connective tissue graft: Histologic and volumetric analyses in a beagle model Heliyon 2023-04-01 [PMID: 37151660] (IHC)	IHC
Cheng Y, Li J, Zhang X et al. Protective Effect of Qingchang Wenzhong Decoction on Colitis and Colitis-Related Carcinogenesis by Regulating Inflammation and Intestinal Fibrosis Journal of inflammation research 2023-04-07 [PMID: 37056910] (IHC-P, Mouse)	IHC-P	Mouse
Li G, Li D, Wu C et al. Homocysteine-targeting compounds as a new treatment strategy for diabetic wounds via inhibition of the histone methyltransferase SET7/9 Experimental & molecular medicine 2022-07-20 [PMID: 35859119] (WB, Human)	WB	Human
Chen y, Chen S, Li T et al. 4-Acetylantroquinonol B enhances cell death and inhibits autophagy by downregulating the PI3K/Akt/MDR1 pathway in gemcitabine-resistant pancreatic cancer cells Oncology Letters 2022-02-18 [PMID: 35251348]
Li D, Fan H, Guo N et al. Xihuang Pill Inhibits The Development of DMBA Combined With Oestrogen- And Progesterone-Induced Precancerous Breast lesions In Rats By The PI3K/AKT/mTOR Signaling Pathway Research Square 2021-09-02 (IHC-P, Rat)	IHC-P	Rat
Feng G, Liu W, Yu Y et al. Angiogenesis coupled with osteogenesis in a bone tissue engineering scaffold enhances bone repair in osteoporotic bone defects Biomedical materials (Bristol, England) 2023-05-05 [PMID: 37144422] (Human)		Human
Liberio BM, Seedorf G, Soranno DE et al. Acute kidney injury decreases pulmonary vascular growth and alveolarization in neonatal rat pups Pediatric research 2023-05-03 [PMID: 37138027]
Kotsaris G, Qazi T, Bucher C et al. Odd skipped-related 1 controls the pro-regenerative response of Fibro-Adipogenic Progenitors bioRxiv 2022-07-04 [PMID: 37019910]
Aoki T, Ono I, Kayahara T et al. Hypoxic microenvironment as a crucial factor triggering events leading to rupture of intracranial aneurysm Research Square 2022-04-07 [PMID: 37015954] (IF/IHC, Rat, Human)	IF/IHC	Rat, Human
Wu M, Yu Z, Matar DY et al. Human Amniotic Membrane Promotes Angiogenesis in an Oxidative Stress Chronic Diabetic Murine Wound Model Advances in wound care 2022-03-16 [PMID: 35293255] (WB, Mouse)	WB	Mouse
Jin Y, Pan Y, Zheng S et al. Inactivation of EGLN3 hydroxylase facilitates Erk3 degradation via autophagy and impedes lung cancer growth Oncogene 2022-02-05 [PMID: 35124697]
Cosgun T, Kisacik O Determination of Nurses' Attitudes towards Nutritional Assessment, Level of Knowledge on Nutritional Care, and Perceived Quality of Care Celal Bayar Universitesi Saglık Bilimleri Enstitusu Dergisi 2021-06-30
Hong Y, Wang Y, Cui Y et al. MicroRNA-124-3p Attenuated Retinal Neovascularization in Oxygen-Induced Retinopathy Mice by Inhibiting the Dysfunction of Retinal Neuroglial Cells through STAT3 Pathway International journal of molecular sciences 2023-07-21 [PMID: 37511525] (IHC, Mouse)	IHC	Mouse
Xiang ZY, Chen SL, Qin XR et al. Changes and related factors of blood CCN1 levels in diabetic patients Frontiers in endocrinology 2023-06-02 [PMID: 37334311] (WB, Rat)	WB	Rat
Xiang X, Zhou L, Lin Z et al. Metformin regulates macrophage polarization via the Shh signaling pathway to improve pulmonary vascular development in bronchopulmonary dysplasia IUBMB life 2021-12-15 [PMID: 34910358] (ELISA, Mouse)	ELISA	Mouse
Saleh T, El-Sadoni M, Alhesa A Et al. Expression of Senescence and Apoptosis Biomarkers in Synchronous Bilateral Breast Cancer: A Case Report Current oncology (Toronto, Ont.) 2021-09-30 [PMID: 34677245] (IF/IHC, Human)	IF/IHC	Human
Li M, Wang Q, Han Q et al. Novel Molecule Nell-1 Promotes the Angiogenic Differentiation of Dental Pulp Stem Cells Frontiers in Physiology 2021-08-26 [PMID: 34512380] (ICC/IF, IHC)	ICC/IF, IHC
de Barros Sene L, Lamana GL, Schwambach Vieira A et al. Gestational Low Protein Diet Modulation on miRNA Transcriptome and Its Target During Fetal and Breastfeeding Nephrogenesis Frontiers in Physiology 2021-06-22 [PMID: 34239447] (IHC)	IHC
Zhu H, Chen D, Xie X et al. Melittin inhibits lung metastasis of human osteosarcoma: Evidence of wnt/beta-catenin signaling pathway participation Toxicon : official journal of the International Society on Toxinology 2021-04-28 [PMID: 33930393]
Mookerjee S Engineering rFVIIa-loaded platelets; a novel approach to treating acute bleeding Thesis 2021-01-01
Doganlar O, Doganlar ZB, Delen E, Dogan A The role of melatonin in angio-miR-associated inhibition of tumorigenesis and invasion in human glioblastoma tumour spheroids Tissue & cell 2021-08-12 [PMID: 34418770]
Zhao Z, Li G, Ruan H Et al. Capturing Magnesium Ions via Microfluidic Hydrogel Microspheres for Promoting Cancellous Bone Regeneration ACS nano 2021-08-03 [PMID: 34342981]
Wang T, Zhang C, Xie H et al. Anti-VEGF therapy prevents MUller intracellular edema by decreasing VEGF-A in diabetic retinopathy Eye and vision (London, England) 2021-04-17 [PMID: 33865457]
Akdas Reis Y, Tapisiz OL, Goktolga U et al. The Effect of Disulfiram in the Prevention of Postoperative Adhesion Formation in an Experimental Rat Uterine Horn Model Reproductive sciences (Thousand Oaks, Calif.) 2021-03-29 [PMID: 33782899] (IF/IHC, Rat)	IF/IHC	Rat
Fan H, Li D, Guo N et al. Xihuang Pill inhibits the development of DMBA combined estrogen and progesterone induced breast precancerous lesions rats by PI3K/AKT/mTOR signaling pathway Research Square 2021-02-10 (IHC-P, Rat)	IHC-P	Rat
Li S, Li Y, Wu Z Et al. Diabetic ferroptosis plays an important role in triggering on inflammation in diabetic wound American journal of physiology. Endocrinology and metabolism 2021-08-23 [PMID: 34423682] (WB, IF/IHC, Rat)	WB, IF/IHC	Rat
Sene LB, Cendes IL, Scarano WR et. al. Impact of gestational low-protein intake on embryonic kidney microRNA expression and in the nephron progenitor cells of the male offspring fetus PLoS One 2021-02-05 [PMID: 33544723]
Bhattacharjee J, Mohammad S, Goudreau AD, Adamo KB Physical activity differentially regulates VEGF, PlGF, and their receptors in the human placenta Physiological reports 2021-01-01 [PMID: 33463910] (IHC-P, Human)	IHC-P	Human
Han X, Sun M, Chen B et al. Lotus seedpod-inspired internal vascularized 3D printed scaffold for bone tissue repair Bioactive Materials 2020-11-07 [PMID: 33313444] (WB)	WB
Cho J, Kim TH, Seok J et al. Vascular remodeling by placenta-derived mesenchymal stem cells restores ovarian function in ovariectomized rat model via the VEGF pathway Laboratory investigation; a journal of technical methods and pathology 2020-12-10 [PMID: 33303971] (WB, Rat)	WB	Rat
So J, Lee J, Ahn Y et al. The synergistic effect of biomimetic electrical stimulation and extracellular-matrix-mimetic nanopattern for upregulating cell activities Biosensors and Bioelectronics 2020-07-01 [PMID: 32823208] (ICC/IF, Mouse) Details: Citation using the Alexa Fluor 488 format of this antibody.	ICC/IF	Mouse
Liu D, Xu H, Zhang C et al. Erythropoietin maintains VE-cadherin expression and barrier function in experimental diabetic retinopathy via inhibiting VEGF/VEGFR2/Src signaling pathway Life Sci. 2020-08-12 [PMID: 32800831]
Bombeiro AL, Pereira BTN, Bonfanti AP, Oliveira ALR. Immunomodulation by dimethyl fumarate treatment improves mouse sciatic nerve regeneration Brain Res Bull. [PMID: 32305403] (FLOW, Mouse)	FLOW	Mouse
Patel, C, Goody, R Et al. Primate model of chronic retinal neovascularization and vascular leakage. Exp Eye Res 2020-06-01 [PMID: 32275921] (Chemotaxis, WB, Mouse)	Chemotaxis, WB	Mouse
Mandell E, Ryan S, Seedorf GJ et al. Maternal Vitamin D Deficiency Causes Sustained Impairment of Lung Structure and Function and Increases Susceptibility to Hyperoxia-Induced Lung Injury in Infant Rats Am. J. Respir. Cell Mol. Biol. 2020-03-05 [PMID: 32135073]
Chou Pr, Lin Yn, Wu Sh et Al. Supercritical Carbon Dioxide-decellularized Porcine Acellular Dermal Matrix combined with Autologous Adipose-derived Stem Cells: Its Role in Accelerated Diabetic Wound Healing Int J Med Sci 2020-02-04 [PMID: 32132871] (IHC-P, Porcine)	IHC-P	Porcine
Li J, Zhou W, Chen W et al. Mechanism of the hypoxia inducible factor 1/hypoxic response element pathway in rat myocardial ischemia/diazoxide post conditioning Mol Med Report 2020-01-28 [PMID: 32016463] (WB, Rat)	WB	Rat
Gao L, Mei S, Zhang S et al. Cardio-renal Exosomes in Myocardial Infarction Serum Regulate Proangiogenic Paracrine Signaling in Adipose Mesenchymal Stem Cells Theranostics 2020-01-01 [PMID: 31938051] (WB, Mouse)	WB	Mouse
Su CC, Hsu TC, Hsiao CH et al. Effects of antibodies against human parvovirus B19 on angiogenic signaling Mol Med Rep 2020-01-09 [PMID: 31922220]
Guo Q, Zhang Y, Zhang S, et al. Genome-wide translational reprogramming of genes important for myocyte functions in overload-induced heart failure Biochim Biophys Acta Mol Basis Dis 2019-12-21 [PMID: 31870714] (WB, Mouse)	WB	Mouse
Wen G, Xu J, Wu T et al. Functionalized polycaprolactone/hydroxyapatite composite microspheres for promoting bone consolidation in a rat distraction osteogenesis model J. Orthop. Res. 2019-11-27 [PMID: 31777101]
Militi A, Cutroneo G, Favaloro A et al. An immunofluorescence study on VEGF and extracellular matrix proteins in human periodontal ligament during tooth movement Heliyon 2019-10-01 [PMID: 31667410] (ICC/IF, Human)	ICC/IF	Human
Lin YC, Chen RY, Liang JA et al. Immunohistochemical Biomarkers of Survival in Patients With Adenocarcinoma of the Uterine Cervix Receiving Chemoradiotherapy Anticancer Res. 2019-06-01 [PMID: 31177173]
Saito H, Tanaka K, Fujiwara M, Iwasaki T et al. Pathological findings of progressive renal involvement in a patient with TAFRO syndrome CEN Case Rep 2019-05-10 [PMID: 31077056]
Dewangan, J;Srivastava, S;Mishra, S;Divakar, A;Kumar, S;Rath, SK; Salinomycin inhibits breast cancer progression via targeting HIF-1 alpha/VEGF mediated tumor angiogenesis in vitro and in vivo Biochem. Pharmacol. 2019-04-24 [PMID: 31028743] (WB, Human)	WB	Human
Shen W, Lee S R et al. A Combination Therapy Targeting Endoglin and VEGF-A Prevents Subretinal Fibro-Neovascularization Caused by Induced MUller Cell Disruption. Invest Ophthalmol Vis Sci 2018-03-12 [PMID: 30592496] (WB, Mouse)	WB	Mouse
Yan Y, Chen H, Zhang H et al. Vascularized 3D printed scaffolds for promoting bone regeneration. Biomaterials. 2018-10-31 [PMID: 30415019] (WB, Rat)	WB	Rat
Chen Y, Gao S, Yan Y, Qian J. Aerosolized deferoxamine administration in mouse model of bronchopulmonary dysplasia improve pulmonary development. Am J Transl Res. 2018-01-15 [PMID: 29423017] (WB, Mouse)	WB	Mouse
Wang C, Wang Q, Gao W et al. Highly efficient local delivery of endothelial progenitor cells significantly potentiates angiogenesis and full-thickness wound healing. Acta Biomater 2018-03-15 [PMID: 29397318]
Xu J, Sun Y, Wu T et al. Enhancement of bone regeneration with the accordion technique via HIF-1alpha/VEGF activation in a rat distraction osteogenesis model J Tissue Eng Regen Med 2017-08-01 [PMID: 28763580] (Rat)		Rat
Farjood Farhad, Vargis Elizabeth Physical disruption of cell-cell contact induces VEGF expression in RPE cells Physical disruption of cell-cell contact induces VEGF expression in RPE cells. Mol Vis. 2017-07-17 [PMID: 28761317] (ICC, Human) Details: Citation using the DyLight 680 version of this antibody.	ICC	Human
Lin Yu-Jung, Shyu Woei-Cherng, Chang Chi-Wei et al. Tumor Hypoxia Regulates Forkhead Box C1 to Promote Lung Cancer Progression. Theranostics 2019-03-05 [PMID: 28435459] (WB, Human)	WB	Human
Du C, Mendelson AA, Guan Q et al. Hyperbranched polyglycerol is superior to glucose for long-term preservation of peritoneal membrane in a rat model of chronic peritoneal dialysis. J Transl Med. 2016-12-13 [PMID: 27964722] (IF/IHC, Rat)	IF/IHC	Rat
Lin YC, Chen RY, Chen SW et al. Immunohistochemical studies and fluorodeoxyglucose uptake on positron emission tomography in pharyngeal cancer for predicting radiotherapy-based treatment outcomes. Clin Otolaryngol. 2016-10-20 [PMID: 27762071] (IF/IHC)	IF/IHC
Su JC, Mar AC, Wu SH et al. Disrupting VEGF-A paracrine and autocrine loops by targeting SHP-1 suppresses triple negative breast cancer metastasis Sci Rep 2016-07-01 [PMID: 27364975] (IF/IHC)	IF/IHC
Chen SW, Lin YC, Chen RY et al. Immunohistochemical overexpression of hypoxia-induced factor 1a associated with slow reduction in (18)fluoro-2-deoxy-D-glucose uptake for chemoradiotherapy in patients with pharyngeal cancer. Eur. J. Nucl. Med. Mol. Imaging 2016-06-16 [PMID: 27311919] (IF/IHC)	IF/IHC
Chen H, Jia P, Kang H et al. Upregulating Hif-1alpha by Hydrogel Nanofibrous Scaffolds for Rapidly Recruiting Angiogenesis Relative Cells in Diabetic Wound. Adv Healthc Mater 2016-04-20 [PMID: 26891197]
Ram M, Singh V, Kumawat S et al. Bilirubin modulated cytokines, growth factors and angiogenesis to improve cutaneous wound healing process in diabetic rats. Int. Immunopharmacol. 2016-01-01 [PMID: 26679676] (WB, Rat) Details: VEGF antibody (clone VG1) was used at 1.5 ug/ml concentration for the analysis of granulation/healing tissues of control and bilirubin-treated diabetic rats (Fig. 4).	WB	Rat
Srinivas C, Ramaiah MJ, Lavanya A et al Novel EPE Analogue Modulates Expression of Angiogenesis Associated microRNAs and Regulates Cell Proliferation by Targeting STAT3 in Breast Cancer PLoS ONE 2015-11-10 [PMID: 26551008] (WB, Human)	WB	Human
Andre H, Tunik S, Aronsson M, Kvanta A. Hypoxia-Inducible Factor-1alpha Is Associated With Sprouting Angiogenesis in the Murine Laser-Induced Choroidal Neovascularization Model. ARVO Journals. 2015-10-01 [PMID: 26501235] (WB, Mouse)	WB	Mouse
Ram M, Singh V, Kumawat S et al. Deferoxamine modulates cytokines and growth factors to accelerate cutaneous wound healing in diabetic rats. Eur J Pharmacol 2015-10-05 [PMID: 26101070] (Rat)		Rat
Shao J, Zhang Y, Yang T et al. HIF-1alpha disturbs osteoblasts and osteoclasts coupling in bone remodeling by up-regulating OPG expression In Vitro Cell. Dev. Biol. Anim. 2015-04-10 [PMID: 25859979] (WB, Mouse)	WB	Mouse
Tam JCW, Ko CH, Lau KM et al. A Chinese 2-herb formula (NF3) promotes hindlimb ischemia-induced neovascularization and wound healing in diabetic rats. Journal of Diabetes and … 2014-03-21 [PMID: 24731763] (IHC-Fr, Rat)	IHC-Fr	Rat
Milanesi A, Lee JW, Li Z et al. Beta-Cell Regeneration Mediated by Human Bone Marrow Mesenchymal Stem Cells PLoS One 2012-01-01 [PMID: 22879915] (IF/IHC, ICC/IF, Mouse)	IF/IHC, ICC/IF	Mouse
Hong DS, Kurzrock R, Oh Y et al. A Phase 1 Dose-Escalation, Pharmacokinetic, and Pharmacodynamic Evaluation of eIF-4E Antisense Oligonucleotide LY2275796 in Patients with Advanced Cancer. Clin Cancer Res. 2011-08-10 [PMID: 21831956]
Ptaszynska MM, Pendrak ML, Bandle RW, Stracke ML, Roberts DD. Positive feedback between vascular endothelial growth factor-A and autotaxin in ovarian cancer cells. Mol Cancer Res;6(3):352-63. 2008-03-01 [PMID: 18337445] (WB, Human)	WB	Human
Couvelard A, O'Toole D, Turley H, Leek R, Sauvanet A, Degott C, Ruszniewski P, Belghiti J, Harris AL, Gatter K, Pezzella F. Microvascular density and hypoxia-inducible factor pathway in pancreatic endocrine tumours: negative correlation of microvascular density and VEGF expression with tumour progression. Br J Cancer;92(1):94-101. 2005-01-17 [PMID: 15558070]
Bhave S, Esposito M, Swain L et al. Loss of Bone Morphogenetic Protein (BMP)-9 Reduces Survival and Increases MMP Activity after Myocardial Infarction JACC: Basic to Translational Science 2023-08-01 (WB, Mouse)	WB	Mouse
Quimbaya P, Garzon V, Bustos R et al. Real-time quantification of proteins secreted of conditioned media from mesenchymal stromal cells (MSC) in co-culture with hematopoietic progenitor cells Sensing and Bio-Sensing Research 2023-11-01 (SPR, Human)	SPR	Human
Parlakgumus H Ayse, Aka Bolat Filiz, Bulgan Kilicdag Esra et al. Atorvastatin for ovarian torsion: effects on follicle counts, AMH, and VEGF expression. Eur J Obstet Gynecol Reprod Biol. 2014-01-20 [PMID: 24507756] (Rat)		Rat
Johansen FF, Hasseldam H, Rasmussen RS et al. Drug-Induced Hypothermia as Beneficial Treatment before and after Cerebral Ischemia. Pathobiology. 2013-08-23 [PMID: 23989388]
Fedorova LV, Sodhi K, Gatto-Weis C et al. Peroxisome Proliferator-Activated Receptor delta Agonist, HPP593, Prevents Renal Necrosis under Chronic Ischemia. PLoS One 2013-05-15 [PMID: 23691217] (WB, Rat)	WB	Rat
Kunze D, Wuttig D, Kausch I et al. Antisense-mediated inhibition of survivin, hTERT and VEGF in bladder cancer cells in vitro and in vivo. Int J Oncol 2008-05-01 [PMID: 18425331] (IHC-P, Mouse)	IHC-P	Mouse
Van der Auwera I, Van Laere SJ, Van den Eynden GG, Benoy I, van Dam P, Colpaert CG, Fox SB, Turley H, Harris AL, Van Marck EA, Vermeulen PB, Dirix LY. Increased angiogenesis and lymphangiogenesis in inflammatory versus noninflammatory breast cancer by real-time reverse transcriptase-PCR gene expression quantification. Clin Cancer Res;10(23):7965-71. 2004-12-01 [PMID: 15585631]
Giatromanolaki A, Koukourakis MI, Simopoulos C, Polychronidis A, Sivridis E. Vascular endothelial growth factor (VEGF) expression in operable gallbladder carcinomas. Eur J Surg Oncol;29(10):879-83. 2003-12-01 [PMID: 14624781]
Kapsoritakis A, Sfiridaki A, Maltezos E, Simopoulos K, Giatromanolaki A, Sivridis E, Koukourakis MI. Vascular endothelial growth factor in inflammatory bowel disease. Int J Colorectal Dis. 18(5):418-22. PubMed PMID: 12761641. 2003-09-01 [PMID: 12761641]
Giatromanolaki A, Koukourakis MI, Sivridis E, Turley H, Wykoff CC, Gatter KC, Harris AL. DEC1 (STRA13) protein expression relates to hypoxia- inducible factor 1-alpha and carbonic anhydrase-9 overexpression in non-small cell lung cancer. J Pathol;200(2):222-8. 2003-06-01 [PMID: 12754744]
Giatromanolaki A, Sivridis E, Maltezos E et al. Hypoxia inducible factor 1alpha and 2alpha overexpression in inflammatory bowel disease. J Clin Pathol. 2003-03-01 [PMID: 12610101]
Salgado R, Benoy I, Weytjens R, Van Bockstaele D, Van Marck E, Huget P, Hoylaerts M, Vermeulen P, Dirix LY. Arterio-venous gradients of IL-6, plasma and serum VEGF and D-dimers in human cancer. Br J Cancer;87(12):1437-44. 2002-12-02 [PMID: 12454774]
Zhang HT, Scott PA, Morbidelli L, Peak S, Moore J, Turley H, Harris AL, Ziche M, Bicknell R. The 121 amino acid isoform of vascular endothelial growth factor is more strongly tumorigenic than other splice variants in vivo. Br J Cancer;83(1):63-8. 2000-07-01 [PMID: 10883669]
Koukourakis MI, Giatromanolaki A, Thorpe PE, Brekken RA, Sivridis E, Kakolyris S, Georgoulias V, Gatter KC, Harris AL. Vascular endothelial growth factor/KDR activated microvessel density versus CD31 standard microvessel density in non-small cell lung cancer. Cancer Res;60(11):3088-95. 2000-06-01 [PMID: 10850461]
Adams J, Carder PJ, Downey S, Forbes MA, MacLennan K, Allgar V, Kaufman S, Hallam S, Bicknell R, Walker JJ, Cairnduff F, Selby PJ, Perren TJ, Lansdown M, Banks RE. Vascular endothelial growth factor (VEGF) in breast cancer: comparison of plasma, serum, and tissue VEGF and microvessel density and effects of tamoxifen. Cancer Res;60(11):2898-905. 2000-06-01 [PMID: 10850435]
Giatromanolaki A, Koukourakis MI, Sivridis E, O'Byrne K, Cox G, Thorpe PE, Gatter KC, Harris AL. Coexpression of MUC1 glycoprotein with multiple angiogenic factors in non-small cell lung cancer suggests coactivation of angiogenic and migration pathways. Clin Cancer Res;6(5):1917-21. 2000-05-01 [PMID: 10815916]
O'Byrne KJ, Koukourakis MI, Giatromanolaki A, Cox G, Turley H, Steward WP, Gatter K, Harris AL. Vascular endothelial growth factor, platelet-derived endothelial cell growth factor and angiogenesis in non-small-cell lung cancer. Br J Cancer;82(8):1427-32. 2000-04-01 [PMID: 10780522] (WB, IHC-P, Human)	WB, IHC-P	Human
Kakolyris S, Giatromanolaki A, Koukourakis M, Leigh IM, Georgoulias V, Kanavaros P, Sivridis E, Gatter KC, Harris AL. Assessment of vascular maturation in non-small cell lung cancer using a novel basement membrane component, LH39: correlation with p53 and angiogenic factor expression. Cancer Res;59(21):5602-7. 1999-11-01 [PMID: 10554041]
Turley H, Scott PA, Watts VM et al. Expression of VEGF in routinely fixed material using a new monoclonal antibody VG1. J Pathol. 1998-11-01 [PMID: 10211122] (IHC-P, ELISA, WB, Human) Details: IF/ICC, (Fig 1.1, 1.2): COS cells transfected with VEGF isoforms 121 and 165, respectively. IHC (Paraffin), Fig 1 (3-8): human peripheral blood, small capillary, ovary, bone marrow, kidney capillaries, endometrial gland, breast, placenta, lung cancer, mel	IHC-P, ELISA, WB	Human
Giatromanolaki A, Koukourakis MI, Kakolyris S, Turley H, O'Byrne K, Scott PA, Pezzella F, Georgoulias V, Harris AL, Gatter KC. Vascular endothelial growth factor, wild-type p53, and angiogenesis in early operable non-small cell lung cancer. Clin Cancer Res;4(12):3017-24. 1998-12-01 [PMID: 9865915] (IF/IHC, Human)	IF/IHC	Human
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Review for VEGF Antibody (NB100-664) (1) 51

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reviewed by:
Anon ymous

Human

02/28/2014

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Application	Western Blot
Sample Tested	HUVEC and 293T cells
Species	Human

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FAQs for VEGF Antibody (NB100-664). (Showing 1 - 6 of 6 FAQs).

Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
- I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.
I would be very grateful if you could let me know a recommended dilution for the VEGF antibody antibody NB100-664
- The recommended dilution for IHC is 1:20-1:50 in an ABC method. If instead you are testing on cell culture, I would recommend a starting optimization point of 1:10 -1:2000.
I would like to know the aproximate dilution of the VEGF Antibody (NB100-664) to use it in a Western blot assay.
- In regards to your inquiry about our VEGF antibody (NB100-664), I am showing from the lab data that they have tested dilutions in the range of 1:500-1:1000 for this antibody. The optimal dilution should depend on your samples and the level of expression of VEGF, but a good starting point would be 1:1000 and then optimize from there.
What is the minimum amount of protein necessary for the detection of VEGF by Western blot using VEGF Antibody (NB100-664)?
- I would suggest at minimum 10ug of protein per well but if you have enough sample you might want to run multiple wells with increasing amounts of protein to optimize the conditions in your hands. You could start with 5ug and do a couple of wells with 10ug, 20ug and 40ug. Also try using different dilutions of primary, start with 1:500-1:1000 and work your way up or down depending on what your blot looks like.
I am interested in buying an antibody to VEGF to detect VEGF in human umbilical vein endothelial cells for western blotting and was wondering if you might have any recommendations? I was thinking about NB100-664. Do you have a recommended amount to use for western blotting? Could I ask you what concentration the antibody is at since it says 0.1mg so what total volume do you send? Also is this the most commonly purchase one for western blotting do you know?
- We do not have a recommended dilution noted for this specific antibody, but our general recommendation for Western blot is 1:1000-1:2000.This is a good starting point, but we always recommend optimization by the end user, as the optimal dilution is to some extent dependent on the particular sample/assay. The concentration for this product is stated as 1 mg/ml, so 0.1 mg would be supplied in 0.1 ml.
I am looking for a VEGF-A antibody for the IHC staining of mouse colon. Do you have any publications or application references for this product?
- NB100-664 is one of our best sellers with great customer feedback and citations in at least 18 peer reviewed publications from highly esteemed researchers. As far as mouse tissues are concerned, I would recommend that you go through the following paper: Milanesi A, et al. Beta-Cell regeneration mediated by human bone marrow mesenchymal stem cells. PLoS One. 2012;7(8):e42177. doi: 10.1371/journal.pone.0042177. Epub 2012 Aug 7. PMID:22879915.

Control Lysate(s)

Human Kidney Whole Tissue Lysate (Adult Whole Normal)
Human Artery Whole Tissue Lysate (Adult Whole Normal)

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Blogs on VEGF. Showing 1-10 of 23 blog posts - Show all blog posts.

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Unlocking the Potential of Biosimilars in Immuno-Oncology
By Jennifer Jones, M.S.Biosimilar Antibodies: Imitation Meets InnovationIn the ever-evolving medical landscape, a new class of pharmaceuticals is emerging as a game-changer, poised to transform the way we approach... Read full blog post.

Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff... Read full blog post.

Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysis
By Rosa Moreno, PhD. Detecting HIF alpha and beyond: Best controls for hypoxia Western blot analysisPhysiological low levels of oxygen induce normal hypoxic events across biological systems. This hypoxic state activ... Read full blog post.

Neurovascular signaling for repair enhances brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Stroke is a leading cause of mortality and morbidity worldwide. Cellular players – neurons, astrocytes, endothelial and stromal cells – involved in post-stroke repair t... Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ... Read full blog post.

Chemotherapy-induced metastasis: An unexpected foe?
By Yoskaly Lazo-Fernandez, PhD IntroductionEvidence has accumulated recently indicating that common cancer therapies might stimulate metastasis in a significant number of cancer patients1. In fact, neoadjuvant che... Read full blog post.

Getting Physical: Link between Lipid Metabolism and Hypoxia Target Genes
By Jamshed Arslan Pharm.D. von Hippel-Lindau (VHL) disease is associated with tumors arising in multiple organs. Activation of hypoxia-inducible factor (HIF)-alpha underlies the VHL disease pathogenesis. In normoxia... Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins. The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein ... Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells. Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol... Read full blog post.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair. Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl... Read full blog post.

Showing 1-10 of 23 blog posts - Show all blog posts.

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Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.

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	Anon ymous 02/28/2014
Application:	WB
Species:	Human

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Bioinformatics

Gene Symbol	VEGFA
Entrez	7422(Human) 22339(Mouse) 83785(Rat)
Uniprot	P15692()

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

VEGF Antibody (VG1) - BSA Free

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Publications for VEGF Antibody (NB100-664)(89)

Review for VEGF Antibody (NB100-664) (1) 51

Summary

Product General Protocols

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FAQs for VEGF Antibody (NB100-664). (Showing 1 - 6 of 6 FAQs).

Control Lysate(s)

Secondary Antibodies

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Additional VEGF Products

Research Areas for VEGF Antibody (NB100-664)

Blogs on VEGF. Showing 1-10 of 23 blog posts - Show all blog posts.

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