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SM047 Antibody (SM047)

Images

 
Western Blot: SM047 Antibody (SM047) [NBP1-97921] - SM047 immunoprecipitates immunoblotted with the SM047 antibody. Lane 1) UWOV1 lysate, Lane 2) ovarian cancer patients serum and Lane 3) pooled normal serum.
Immunocytochemistry/ Immunofluorescence: SM047 Antibody (SM047) [NBP1-97921] - Staining of a cell from an ovarian cancer cell line, labeled with SMO47 antibody. The green fluorescence shows where the antibody has bound ...read more
Immunohistochemistry: SM047 Antibody (SM047) [NBP1-97921] - Immunohistochemical analysis of SM047 in human ovarian cancer using NBP1-97921. Trypsin antigen retrieval was preformed (0.1%, 37C 10 min).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
SM047
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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SM047 Antibody (SM047) Summary

Immunogen
Cell membranes from human ovarian serous cystadenocarcinmoma and cells from human ovarian cancer cell line UWOV1.
Specificity
Glycocalyx of human ovarian carcinoma cells.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10-1:100
  • Western Blot 1:1000-1:5000
Application Notes
This SM047 antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In Western blot a band can be seen above 200 kDa. For IHC-P, trypsin antigen retrieval is recommended (0.1%, 37C 10 min).
Publications
Read Publication using NBP1-97921.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for SM047 Antibody (SM047)

  • anti-SMO47 antibody

Background

SM047 is a monoclonal antibody that binds to an epitope on a multivalent antigen which is associated with the glycocalyx of ovarian carcinoma (OvC) cells and a limited variety of other malignant and normal tissue. SM047 is strongly expressed in most ovarian serous adenocarcinomas and in other female genital tract adenocarcinomas. However, it does not react with ovarian mucinous tumors. The SM047 antibody may be useful in confirming the origin of an ovarian adenocarcinoma. See PMID: 11422498 for additional information on SM047.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SM047 Antibody (NBP1-97921)(1)

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Product General Protocols

View specific protocols for SM047 Antibody (NBP1-97921): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SM047 Antibody (NBP1-97921) (0)

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Secondary Antibodies

 

Isotype Controls

Additional SM047 Products

Array NBP1-97921

Research Areas for SM047 Antibody (NBP1-97921)

Find related products by research area.

Blogs on SM047.

SM047: A Powerful Ovarian Carcinoma Marker
The SM047 antibody is an IgM monoclonal antibody that was developed by McCluggage's group at the UK Royal Group of Hospitals and recognizes a multivalent antigen from ovarian carcinoma (OvC) cells (1). Early studies indicate that the epitope is speci...  Read full blog post.

SM047: A Marker for Ovarian Adenocarcinoma
Glandular tissues synthesise substances for release, such as hormones and enzymes. The term adenocarcinoma is used to describe a cancer that originates within a glandular tissue, for example the ovary.The incidence of ovarian cancer is low, yet ovar...  Read full blog post.

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