I ordered the MMP-2 ms monoclonal ab 8B4, and after several tries, I cannot get it to work. I have tried in my cell lysates, concentrated conditioned media and a uterine tissue, and I am still not able to see it. Would it be possible for your company to send me a lysate that I can use as a positive control so that I can test it in something where it should be expressed? Also, are there any already known conditions for western blotting?

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Accepted Answer

Our lab does not have any of the positive control biopsy lysate available to send you as a control. We do offer some transfected lysates that may be of interest to you. I also have some additional information about expected samples that would express the protein. You may also find this information on UniProt useful.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, ELISA, ICC/IF, IHC, IP, KD
Clone	8B4
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Format	BSA Free
Concentration	1 mg/ml

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB200-114AF350

Alexa Fluor 405 NB200-114AF405

Alexa Fluor 488 NB200-114AF488

Alexa Fluor 532 NB200-114AF532

Alexa Fluor 594 NB200-114AF594

Alexa Fluor 647 NB200-114AF647

Alexa Fluor 700 NB200-114AF700

Alexa Fluor 750 NB200-114AF750

Biotin NB200-114B

DyLight 350 NB200-114UV

DyLight 405 NB200-114V

DyLight 488 NB200-114G

DyLight 550 NB200-114R

DyLight 594 NB200-114DL594

DyLight 650 NB200-114C

DyLight 680 NB200-114FR

DyLight 755 NB200-114IR

FITC NB200-114F

HRP NB200-114H

Janelia Fluor 549 NB200-114JF549

Janelia Fluor 646 NB200-114JF646

mFluor Violet 450 SE NB200-114MFV450

mFluor Violet 500 SE NB200-114MFV500

mFluor Violet 610 SE NB200-114MFV610

Catalog# & Formulation	Size	Price
BSA Free NB200-114SS

Azide and BSA Free NBP2-80854

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB200-114AF350
Alexa Fluor 405	NB200-114AF405
Alexa Fluor 488	NB200-114AF488
Alexa Fluor 532	NB200-114AF532
Alexa Fluor 594	NB200-114AF594
Alexa Fluor 647	NB200-114AF647
Alexa Fluor 700	NB200-114AF700
Alexa Fluor 750	NB200-114AF750
Biotin	NB200-114B
DyLight 350	NB200-114UV
DyLight 405	NB200-114V
DyLight 488	NB200-114G
DyLight 550	NB200-114R
DyLight 594	NB200-114DL594
DyLight 650	NB200-114C
DyLight 680	NB200-114FR
DyLight 755	NB200-114IR
FITC	NB200-114F
HRP	NB200-114H
Janelia Fluor 549	NB200-114JF549
Janelia Fluor 646	NB200-114JF646
mFluor Violet 450 SE	NB200-114MFV450
mFluor Violet 500 SE	NB200-114MFV500
mFluor Violet 610 SE	NB200-114MFV610

MMP-2 Antibody (8B4) - (Pro and Active) - BSA Free

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Alternate Names for MMP-2 Antibody (8B4) - (Pro and Active) - BSA Free

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Publications for MMP-2 Antibody (NB200-114)(21)

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Immunogen	Activated recombinant human MMP-2. [Uniprot: P08253]
Specificity	This is specific for pro and active MMP2.
Isotype	IgG1 Kappa
Clonality	Monoclonal
Host	Mouse
Gene	MMP2
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.05% Sodium Azide
Concentration	1 mg/ml
Purity	Protein G purified

Publications using NB200-114	Applications	Species
Sun DP, Huang HY, Chou CL et al. Punicalagin is cytotoxic to human colon cancer cells by modulating cell proliferation, apoptosis, and invasion Human & experimental toxicology 2023-11-07 [PMID: 37933160] (WB, Human)	WB	Human
Gerwinn T, Salemi S, Schori LJ et al. Improved contractile potential in detrusor microtissues from pediatric patients with end stage lower urinary tract dysfunction Frontiers in Cell and Developmental Biology 2022-10-04 [PMID: 36268506] (ICC/IF)	ICC/IF
DIACONU GS, MIHALACHE CG, POPESCU G Clinical and pathological considerations in lumbar herniated disc associated with inflammatory lesions rjme.ro 2021-01-01 [PMID: 35673814] (IF/IHC, Human)	IF/IHC	Human
Neff LS, Zhang Y, Van Laer AO et al. Mechanisms that limit regression of myocardial fibrosis following removal of left ventricular pressure overload American journal of physiology. Heart and circulatory physiology 2022-07-01 [PMID: 35657618]
Damerell V, Ambele MA, Salisbury S et al. The c-Myc/TBX3 Axis Promotes Cellular Transformation of Sarcoma-Initiating Cells Frontiers in Oncology 2022-01-25 [PMID: 35145908] (WB)	WB
PREDA SA, PREDESCU AM, STOICA LE Histopathological and immunohistochemical changes of the marginal periodontium in patients with Turner syndrome changes 2021-01-01 [PMID: 34609427] (IF/IHC, Human)	IF/IHC	Human
Betsou A, Lambropoulou M, Georgakopoulou AE et al. The hepatoprotective effect of silibinin after hepatic ischemia/reperfusion in a rat model is confirmed by immunohistochemistry and qRT-PCR The Journal of pharmacy and pharmacology 2021-04-13 [PMID: 33847359]
Perek B, Kowalska K et al. Infiltration of CD68+ cells correlates positively with matrix metalloproteinase 2 expression in the arteries used as aortocoronary bypass grafts. Possible clinical implications. Cardiol J 2019-06-09 [PMID: 31489609] (IF/IHC, Human)	IF/IHC	Human
Shang S, Ji X, Zhang L et al. Macrophage ABHD5 suppresses NF-kappa B-dependent matrix metalloproteinase expression and cancer metastasis Cancer Res. 2019-08-22 [PMID: 31439546] (KD, Mouse)	KD	Mouse
Thompson PJ, Shah A, Ntranos V et al. Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes Cell Metab. 2019-02-14 [PMID: 30799288] (IF/IHC, Mouse)	IF/IHC	Mouse
Erikson JM, Valente AJ, Mummidi S et al. Targeting TRAF3IP2 by genetic and interventional approaches inhibits ischemia/reperfusion-induced myocardial injury and adverse remodeling J. Biol. Chem 2017-01-04 [PMID: 28053087] (WB, Mouse)	WB	Mouse
Sakamuri SS, Valente AJ, Siddesha JM et al. TRAF3IP2 mediates aldosterone/salt-induced cardiac hypertrophy and fibrosis. Mol. Cell. Endocrinol. 2016-04-01 [PMID: 27040306] (WB, Mouse)	WB	Mouse
Siddesha Jalahalli M, Valente Anthony J, Yoshida Tadashi et al. Docosahexaenoic acid reverses angiotensin II-induced RECK suppression and cardiac fibroblast migration. Cell Signal. 2014-01-19 [PMID: 24447911] (WB, Mouse)	WB	Mouse
Huang L, Cai YJ, Lung I et al. A study of the combination of triamcinolone and 5-fluorouracil in modulating keloid fibroblasts in vitro. J Plast Reconstr Aesthet Surg 2013-06-27 [PMID: 23810214] (WB, Human)	WB	Human
Raaf L, Noll C, Cherifi Mel H et al. Myocardial fibrosis and TGFB expression in hyperhomocysteinemic rats. Mol Cell Biochem 347(1-2):63-70. 2011-01-01 [PMID: 20938722] (WB, Mouse)	WB	Mouse
von Leithner PL, Ciurtin C, Jeffery G. Microscopic mammalian retinal pigment epithelium lesions induce widespread proliferation with differences in magnitude between center and periphery. Mol Vis 16:570-81. 2010-03-31 [PMID: 20360994] (ICC/IF, IF/IHC, Rat)	ICC/IF, IF/IHC	Rat
Qian J, Dong A, Kong M et al. Suppression of type 1 Insulin-like growth factor receptor expression by small interfering RNA inhibits A549 human lung cancer cell invasion in vitro and metastasis in xenograft nude mice. Acta Biochim Biophys Sin (Shanghai). [PMID: 17277889] (WB, Mouse)	WB	Mouse
Locke NR, Royce SG, Wainewright JS et al. Comparison of airway remodeling in acute, subacute, and chronic models of allergic airways disease. Am J Respir Cell Mol Biol 36(5):625-32. 2007-05-01 [PMID: 17237192] (IHC-P, Mouse)	IHC-P	Mouse
Ribeiro-Silva A, Ribeiro do Vale F, Zucoloto S. Vascular endothelial growth factor expression in the basal subtype of breast carcinoma. Am J Clin Pathol 125(4):512-8. 2006-04-01 [PMID: 16627261] (IHC-P, Human)	IHC-P	Human
Wang XM, Yu DM, McCaughan GW et al. Fibroblast activation protein increases apoptosis, cell adhesion, and migration by the LX-2 human stellate cell line. Hepatology 42(4):935-45. 2005-10-01 [PMID: 16175601] (ICC/IF, Human)	ICC/IF	Human
Dong B, Sato M, Sakurada A et al. Computed tomographic images reflect the biologic behavior of small lung adenocarcinoma: they correlate with cell proliferation, microvascularization, cell adhesion, degradation of extracellular matrix, and K-ras mutation. J Thorac Cardiovasc Surg 130(3):733-9. 2005-09-01 [PMID: 16153921] (IHC-P, Human)	IHC-P	Human
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