BAG3 Antibody

The BAG proteins are a family of chaperone regulators that modulate a number of diverse processes including proliferation, survival, stress responses, tumorigenesis, neuronal differentiation, growth arrest and apoptosis (reviewed Takayama and Reed, 2001; Doong et al, 2002, and Doukhanina et al. 2006). BAG proteins have been characterized as co-chaperones and interact with the chaperone heat shock proteins 70, both constitutive Hsc70 and inducible Hsp70. BAG proteins bind through their BAG domain to the ATPase domain of Hsc70/Hsp70, and can modulate either positively or negatively the functions of the Hsc70/Hsp70 chaperone proteins. The BAG domain has been shown to contribute to the anti-apoptotic activity of BAG- family proteins. The anti-apoptotic activities of BAG-family proteins may be dependent on their interactions with Hsc70/Asp70 and/or binding to Bcl-2. In addition to the conserved BAG domain, BAG-family proteins also contain additional domains which enable them to interact with specific target proteins or to target them to specific locations within cells. The BAG family contains at least six family members, including BAG-1 and its various isoforms [including BAG-1S , BAG-1M (RAP46/HAP46), and BAG-1L, BAG2, BAG3 (CAIR-1; Bis,), BAG4 (SODD), BAG5 and BAG6 (Scythe, BAT3). Increased BAG-3 expression has been described in pancretic cancer compared to normal pancreas, Detectible BAG-3 expression has also been described inserveral pancreatric cancer cell lines. Please see Liao et al (2001) for details. In normal organs, BAG-3 protein has been found to be abundantly expressed in muscle. Please see Homma et al for details. The following amino acids (aa) lengths and molecular weights (kDa) have been described for human BAG proteins (reviewed in Takayama et al, 2001 and Doong et al, 2002): BAG-1 (230 aa., 34 kDa), BAG-1S (219 aa, 29 kDa), BAG-1M (274 aa, 46 kDa), BAG-1L (345 aa, 52 kDa), BAG-2 [212 aa; 24 kDa (Arndt et al. 2005)], BAG-3 (575 aa, 74 kDa), BAG-4 (456 aa; 60 kDa), BAG-5 ([442 aa; 51 kDa (kalia et al. 2004)], and BAG-6 (1129 aa; 150 kDa). IMG-5668 recognizes both BAG-3; BAG-3 migrates at ~74-80 kDa on SDS-PAGE.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.

Showing Publications 1 - 10 of 13. Show All 13 Publications. Collapse Publications.

Publications using NBP2-27398	Applications	Species
Damiani V, Lamolinara A, Cicalini I et al. Pancreatic beta-cell specific BAG3 knockout results in chronic hyperinsulinemia inducing insulin resistance Molecular metabolism 2023-06-10 [PMID: 37308077] (ICC/IF, Mouse)	ICC/IF	Mouse
Lu S, Hu J, Arogundade OA et al. Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition Nature Cell Biology 2022-09-08 [PMID: 36075972]
Damiani V, Cufaro MC, Fucito M et al. Proteomics Approach Highlights Early Changes in Human Fibroblasts-Pancreatic Ductal Adenocarcinoma Cells Crosstalk Cells 2022-03-29 [PMID: 35406724] (WB, Human)	WB	Human
Yapa Abeywardana M, Samarasinghe KTG, Munkanatta Godage D, Ahn YH Identification and Quantification of Glutathionylated Cysteines under Ischemic Stress Journal of proteome research 2021-08-12 [PMID: 34382403]
Li X, Lin G, Liu T Et al. Postnatal development of BAG3 expression in mouse cerebral cortex and hippocampus Brain structure & function 2021-08-06 [PMID: 34357438]
Mukherjee, T, Ramaglia, V Et al. The eIF2 alpha kinase HRI triggers the autophagic clearance of cytosolic protein aggregates. J Biol Chem 2020-12-02 [PMID: 33410405] (WB, Mouse)	WB	Mouse
Chien CY, Wen TJ, Cheng YH et al. Diabetes Upregulates Oxidative Stress and Downregulates Cardiac Protection to Exacerbate Myocardial Ischemia/Reperfusion Injury in Rats Antioxidants (Basel) 2020-07-29 [PMID: 32751309] (IHC-P, WB, Rat)	IHC-P, WB	Rat
Ormeno F, Hormazabal J, Moreno J et Al. Chaperone Mediated Autophagy Degrades TDP-43 Protein and Is Affected by TDP-43 Aggregation Front Mol Neurosci 2020-02-18 [PMID: 32132902] (WB, Human, Rat)	WB	Human, Rat
Li X, Lu J, Kan Q et al. Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells Sci Rep 2017-07-11 [PMID: 28698547] (WB, Human)	WB	Human
Aust Stefanie, Pils Sophie, Polterauer Stefan et al. Expression of Bcl-2 and the antiapoptotic BAG family proteins in ovarian cancer. Appl Immunohistochem Mol Morphol. 2013-12-01 [PMID: 23455184] (IHC-P, Human)	IHC-P	Human
Zhang Y, Wang JH, Lu Q, Wang YJ. Bag3 promotes resistance to apoptosis through Bcl-2 family members in non-small cell lung cancer. Oncol Rep. 2012-01-01 [PMID: 21971866]
Goldman A, Chen HD, Roesly HB et al. Characterization of squamous esophageal cells resistant to bile acids at acidic pH: implication for Barrett's esophagus pathogenesis. Am J Physiol Gastrointest Liver Physiol. 2011-02-01 [PMID: 21127259] (WB, Human) Details: BAG-3 (IMG-5676). WB: Normal human esophageal epithelial cell lines (HET1A & HET1AR), Fig 4D.	WB	Human
Liao Q, Ozawa F, Friess H et al. The anti-apoptotic protein BAG-3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines. FEBS Lett. 2001-08-17 [PMID: 11513873] (IHC-P, WB, Human) Details: WB: human tissues and cell lines (Figs 1, 4-6); IHC (P): human pancreas tissue (Fig 2).	IHC-P, WB	Human
Show All 13 Publications. Collapse Publications.

Array NBP2-27398

• BAG3 Antibodies
• BAG3 ELISA Kit
• BAG3 Proteins