AbSelect™ Antibody Concentration and Clean Up Antibody Purification Kit Summary
| Description |
Antibodies are sometimes only available at low concentrations and often contain low molecular weight substances that interfere in labeling reactions with enzymes, biotin, streptavidin and fluorophores. | Features | Benefits | | Quickly and easily concentrate antibodies and proteins | Easy and quick to use | | Remove non-protein additives such as sodium azide, tris and glycine | High recoveries, around 60 - 100% | | Perform a simple buffer exchange for more favourable conjugation conditions | Suitable for Lightning-Link kits and for other kits |
The antibody concentration and clean-up kit method utilizes a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution. |
| Kit Type |
Antibody Purification Kit |
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze. |
Kit Components
|
Components
|
- 1 bottle of AbSelect Conjugation buffer
- 3 spin cartidges / collecting tube assemblies
|
Notes
This product is manufactured by Expedeon Inc. and distributed by Novus Biologicals.
Background
The Antibody concentration and Clean Up Kit allows for the quick and easy concentration of antibodies and proteins. Antibodies are sometimes only available at low concentrations and often contain low molecular weight substances that interfere in labeling reactions with enzymes, biotin, streptavidin and fluorophores. The kit can also be used to reduce the concentration of many unwanted additives often found in antibody formulations such as azide, glycine or tris. The antibody clean up kit method utilizes a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution. The clean up kit also allows the experimenter to perform a simple buffer exchange to transfer the antibody into a more favorable conjugation buffer.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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