AbSelect™ TCS Purification System Antibody Purification Kit Summary
| Description |
Antibodies are often generated from hybridoma cell lines and supplied in tissue culture supernatant (TCS). TCS itself contains many substances that interfere with antibody labeling reactions. Successful labeling therefore requires antibodies to be purified prior to labeling. The AbSelect TCS system is a fast and simple method to purify these antibodies. AbSelect TCS is prepared by coupling highly purified protein A to agarose beads which can be used to purify IgG fractions from hybridoma supernatants. The method involves the capture of the antibody on the AbSelect TCS resin and the removal of unwanted substances using a simple wash procedure. The purified product is then eluted and neutralized. The components of the AbSelect TCS are fully compatible with the Lightning-Link antibody labeling kits (available separately), which allows the purified antibody to be immediately labeled with an enzyme, fluorescent protein or fluorescent dye, with a hands-on time of under 30 seconds. Benefits: - All components and buffers supplied
- Purify antibody from 10 - 50 mls of hybridoma TCS
|
| Kit Type |
Antibody Purification Kit |
Applications/Dilutions
| Application Notes |
The antibody to be purified should be in 10 to 50 mL of tissue culture supernatant. Up to 5 mg of antibody can be purified in each run. |
Packaging, Storage & Formulations
Kit Components
|
Components
|
- AbSelect™ TCS Protein A Resin (1 vial)
- Binding buffer - 10X (1 bottle)
- Concentrator spin column and collection tube (1 EA)
- Elution buffer (1 bottle)
- Neutralization buffer (1 vial)
- Purification column (1 EA)
- Wash buffer (1 bottle)
|
Notes
AbSelect® is a registered trademark of Abcam and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.
Background
Antibodies are often generated from hybridoma cell lines and supplied in tissue culture supernatant (TCS). TCS itself contains many substances that interfere with antibody labeling reactions. Successful labeling therefore requires antibodies to be purified prior to labeling. The AbSelect TCS system is a fast and simple method to purify these antibodies. The components of the AbSelect TCS are fully compatible with the Lightning-Link antibody labeling kits (available separately), which allows the purified antibody to be immediately labeled with an enzyme, fluorescent protein or fluorescent dye, with a hands-on time of under 30 seconds.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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