AbSelect™ Generic Antibody Purification Kit Summary
| Description |
Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions. The AbSelect Purification Kit quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum.
The antibody to be purified or cleaned up ideally is in a volume of 100 ul to 0.5 ml. Up to 500 ug of antibody can be purified in each run. The AbSelect method involves capture of the antibody on protein A resin and the removal of unwanted substances by a simple wash procedure. The purified product is then eluted and neutralized.
The components of the AbSelect kit are fully compatible with the Lightning-Link antibody labeling system (available separately), which allows the purified antibody to be rapidly conjugated with a wide variety of labels. |
| Kit Type |
Antibody Purification Kit |
Applications/Dilutions
| Application Notes |
The volume of antibody to be purified or cleaned up should be 0.1-0.5 mL, though larger volumes may be processed by first incubating the antibody sample (combined with the Binding Buffer) with the Protein A resin in a larger vessel (e.g. 2 mL-eppendorf tube) prior to transferring to the spin cartridge in several aliquots, spinning down the excess liquid each time. |
Packaging, Storage & Formulations
Kit Components
|
Components
|
- 1 or 3 spin cartridge / collecting tube assemblies
- 1 or 3 vials of AbSelect Protein A resin
- 1 vial of 10x Binding buffer
- 1 vial of Elution buffer
- 1 vial of Neutralization buffer
- 1 vial of Washing buffer
- 4 or 12 additional collecting tubes
|
Notes
AbSelect® is a registered trademark of Abcam and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.
Background
Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. The AbSelect(TM) Purification Kit quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up ideally is in a volume of 100ul to 0.5ml. Up to 500ug of antibody can be purified in each run. The AbSelect method involves capture of the antibody on protein A resin and the removal of unwanted substances by a simple wash procedure. The purified product is then eluted and neutralized. The components of the AbSelect kit are fully compatible with the Lightning-Link(TM) conjugation system (available separately), which allows the purified antibody to be rapidly conjugated with a wide variety of labels.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
Publications for AbSelect (TM) Generic Kit (860-0010) (0)
There are no publications for AbSelect (TM) Generic Kit (860-0010).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for AbSelect (TM) Generic Kit (860-0010) (0)
There are no reviews for AbSelect (TM) Generic Kit (860-0010).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Additional AbSelect (TM) Generic Products
Blogs on AbSelect (TM) Generic
