ASC/TMS1 Antibody - BSA Free

ASC (apoptosis-associated speck-like protein containing a CARD), also known as TMS1 (target of methylation-induced silencing), was first identified in 1999 as a protein that forms aggregates, or specks, during retinoic acid-induced apoptosis in a human leukemia cell line (1). Furthermore, it was discovered to have a role as a tumor suppressor as methylation silences ASC/TMS1 expression in many tumors (2-5). ASC/TMS1 is synthesized as a 195-amino acid (aa) protein with a theoretical weight of 22 kDa. Structurally the protein contains a N-terminal PYD (pyrin domain) and C-terminal CARD (caspase-recruitment domain) (1-4). Historically, CARD and PYD-containing proteins are known to have crucial functions in regulating apoptosis and immune response pathways (2-5). Furthermore, mutations in many CARD and PYD-containing proteins have been linked to various cancers and inflammatory diseases (2-5). Given its immune response role, it is not surprising that ASC/TMS1 is typically highly expressed in immune cells, specifically in neutrophils and cells of the macrophage/monocyte lineage (5). Additionally, it is expressed in many normal epithelial cell types (4,5).

In regard to immune and inflammatory response, ASC/TMS1 is involved in inflammasome function (3-4). The inflammasome is a multiprotein complex that responds to cellular stress or pathogens and activates inflammatory responses. Specifically, ASC/TMS1 helps assemble the NLRP3 inflammasome complex which then activates caspase-1, followed by stimulation of proinflammatory cytokines including IL-1b and IL-18 (3-4). In terms of the role in regulating apoptosis, multiple studies have revealed that the ASC/TMS1 gene is hypermethylated in many cancers including breast, lung, glioblastomas, and melanomas (2-5). The increased methylation results in decreased gene expression, or silencing, allowing those cancer cells to escape apoptosis (2-5).

References

1. Masumoto, J., Taniguchi, S., Ayukawa, K., Sarvotham, H., Kishino, T., Niikawa, N., Hidaka, E., Katsuyama, T., Higuchi, T., & Sagara, J. (1999). ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells. The Journal of biological chemistry, 274(48), 33835-33838. https://doi.org/10.1074/jbc.274.48.33835

2. McConnell, B. B., & Vertino, P. M. (2004). TMS1/ASC: the cancer connection. Apoptosis: an international journal on programmed cell death. https://doi.org/10.1023/B:APPT.0000012117.32430.0c

3. Salminen, A., Kauppinen, A., Hiltunen, M., & Kaarniranta, K. (2014). Epigenetic regulation of ASC/TMS1 expression: potential role in apoptosis and inflammasome function. Cellular and molecular life sciences : CMLS. https://doi.org/10.1007/s00018-013-1524-9

4. Protti, M. P., & De Monte, L. (2020). Dual Role of Inflammasome Adaptor ASC in Cancer. Frontiers in cell and developmental biology. https://doi.org/10.3389/fcell.2020.00040

5. Parsons, M. J., & Vertino, P. M. (2006). Dual role of TMS1/ASC in death receptor signaling. Oncogene. https://doi.org/10.1038/sj.onc.1209684

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 12 applications: B/N, ELISA, FLOW, Flow Cytometry Control, ICC/IF, IF/IHC, IHC, IHC-P, IM, IP, In Vivo, WB.

Showing Publications 1 - 10 of 45. Show All 45 Publications. Collapse Publications.

Publications using NBP1-78977	Applications	Species
Jing X, Luo X, Fang C, Zhang B N-acetylserotonin inhibits oxidized mitochondrial DNA-induced neuroinflammation by activating the AMPK/PGC-1?/TFAM pathway in neonatal hypoxic-ischemic brain injury model International Immunopharmacology 2023-03-01 (WB, Rat)	WB	Rat
Quan JH, Gao FF, Ma TZ et al. Toxoplasma gondii induces pyroptosis in human placental trophoblast and amniotic cells by inducing ROS production and activation of cathepsin B and NLRP1/NLRP3/NLRC4/AIM2 inflammasome The American journal of pathology 2023-09-21 [PMID: 37741453] (IHC-P, Mouse)	IHC-P	Mouse
Ning J, Pei Z, Wang M et al. Site-specific Atg13 methylation-mediated autophagy regulates epithelial inflammation in PM2.5-induced pulmonary fibrosis Journal of hazardous materials 2023-09-05 [PMID: 37295326]
Li T, Li L, Peng R et al. Abrocitinib Attenuates Microglia-Mediated Neuroinflammation after Traumatic Brain Injury via Inhibiting the JAK1/STAT1/NF-kappa B Pathway Cells 2022-11-13 [PMID: 36429017] (WB, Mouse)	WB	Mouse
Zheng J, Pang Y, Zhang Y et al. Indoor VOCs exposure induced Parkinson-like behaviors through autophagy dysfunction and NLRP3 inflammasome-mediated neuroinflammation Journal of Hazardous Materials 2022-10-01 (ICC/IF, WB, Mouse) Details: ICC/IF Dilutions: 1:200; WB Dilutions: 1:1000	ICC/IF, WB	Mouse
Shih H, Chang C, Lai C, Huang C Therapeutic effect of modulating the NLRP3-regulated transforming growth factor-beta signaling pathway on interstitial cystitis/bladder pain syndrome Biomedicine & Pharmacotherapy Jun 1 2021 (IHC-P, Mouse)	IHC-P	Mouse
Long J, Sun Y, Liu S et al. Ginsenoside Rg1 treats ischemic stroke by regulating CKLF1/CCR5 axis-induced neuronal cell pyroptosis Phytomedicine 2023-11-01 [PMID: 38128394] (WB, Rat)	WB	Rat
Ivarsson J, Ferrara F, Vallese A et al. Comparison of Pollutant Effects on Cutaneous Inflammasomes Activation International Journal of Molecular Sciences 2023-11-23 [PMID: 38068996] (WB, IHC, Human)	WB, IHC	Human
McElwain CJ, Musumeci A, Manna S et al. L-ergothioneine reduces mitochondrial-driven NLRP3 activation in gestational diabetes mellitus Journal of reproductive immunology 2023-11-24 [PMID: 38029485] (WB, Human)	WB	Human
Gratuze M, Schlachetzki JCM, D'Oliveira Albanus R et al. TREM2-independent microgliosis promotes tau-mediated neurodegeneration in the presence of ApoE4 Neuron 2022-11-04 [PMID: 36368315] (WB, Mouse) Details: Dilution used in WB 1:1000	WB	Mouse
Ferrara F, Cordone V, Pecorelli A et al. Ubiquitination as a key regulatory mechanism for O3-induced cutaneous redox inflammasome activation Redox biology 2022-08-21 [PMID: 36027676] (ICC/IF, WB, Human) Details: ICC/IF Dilutions: 1:100; WB Dilutions: 1:1000	ICC/IF, WB	Human
Zaninelli TH, Fattori V, Saraiva-Santos T et al. RvD1 disrupts nociceptor neuron and macrophage activation, and neuroimmune communication reducing pain and inflammation in gouty arthritis in mice British journal of pharmacology 2022-06-18 [PMID: 35716378]
Zhang ZY, Dang SP, Li SS et al. Glucose Fluctuations Aggravate Myocardial Fibrosis via the Nuclear Factor-?B-Mediated Nucleotide-Binding Oligomerization Domain-Like Receptor Protein 3 Inflammasome Activation Frontiers in Cardiovascular Medicine 2022-05-03 [PMID: 35592403] (WB, B/N)	WB, B/N
Jung ES, Suh K, Han J et al. Amyloid-beta activates NLRP3 inflammasomes by affecting microglial immunometabolism through the Syk-AMPK pathway Aging cell 2022-04-27 [PMID: 35474599] (IF/IHC, WB, ICC/IF, Mouse)	IF/IHC, WB, ICC/IF	Mouse
Chen XC, Wu D, Wu HL et al. Metformin improves renal injury of MRL/lpr lupus-prone mice via the AMPK/STAT3 pathway Lupus Science & Medicine 2022-04-11 [PMID: 35414608] (In Vivo)	In Vivo
Lage SL, Amaral EP, Hilligan KL et al. Persistent Oxidative Stress and Inflammasome Activation in CD14highCD16- Monocytes From COVID-19 Patients Frontiers in immunology 2022-01-14 [PMID: 35095880] (Flow Cytometry Control, Human)	Flow Cytometry Control	Human
Zhang C, Wang X, Wang C et al. Qingwenzhike Prescription Alleviates Acute Lung Injury Induced by LPS via Inhibiting TLR4/NF-kB Pathway and NLRP3 Inflammasome Activation Frontiers in Pharmacology 2021-12-23 [PMID: 35002723] (WB, ELISA)	WB, ELISA
Hosoya T, Uchida S, Shibata S Et al. Xanthine Oxidoreductase Inhibitors Suppress the Onset of Exercise-induced AKI in High HPRT Activity Urat1-Uox Double Knockout Mice Journal of the American Society of Nephrology : JASN 2021-11-19 [PMID: 34799437] (WB, Mouse)	WB	Mouse
Pastar I, Sawaya AP, Marjanovic J Et al. Intracellular Staphylococcus aureus triggers pyroptosis and contributes to inhibition of healing due to Perforin-2 suppression The Journal of clinical investigation 2021-11-02 [PMID: 34730110] (WB, Mouse, Human)	WB	Mouse, Human
Li Z, Xu H, Xu Y et al. Morinda officinalis oligosaccharides alleviate depressive-like behaviors in post-stroke rats via suppressing NLRP3 inflammasome to inhibit hippocampal inflammation CNS Neurosci Ther 2021-09-24 [PMID: 34559953]
Li F, Wang C, Wang J et al. Resveratrol Attenuates Exercise-induced Acute Kidney Injury by Inhibiting NLRP3 Inflammasome-mediated Renal Tubular Pyroptosis Research Square 2023-07-24 (ICC/IF, WB, Rat)	ICC/IF, WB	Rat
Guo ML, Chivero ET, Callen SE, Buch S NLRP3 Inflammasome Is Involved in Cocaine-Mediated Potentiation on Behavioral Changes in CX3CR1-Deficient Mice Journal of personalized medicine 2021-09-27 [PMID: 34683104] (WB, Mouse)	WB	Mouse
Dang R, Yang M, Cui C et al. Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling Aging cell 2021-09-16 [PMID: 34529881] (IHC-P, WB, Mouse)	IHC-P, WB	Mouse
Li X, Zhang X, Xia J Et al. Macrophage HIF-2 alpha suppresses NLRP3 inflammasome activation and alleviates insulin resistance Cell reports 2021-08-24 [PMID: 34433035] (ICC/IF)	ICC/IF
Zhang XL, Huang WM, Tang PC et al. Anti-inflammatory and neuroprotective effects of natural cordycepin in rotenone-induced PD models through inhibiting Drp1-mediated mitochondrial fission Neurotoxicology 2021-02-04 [PMID: 33549657] (WB, Rat)	WB	Rat
Gordon M, Meagher P, Connelly K The Effect of Empagliflozin and Liraglutide on the NLRP3 Inflammasome in a rodent model of type 2 Diabetes Mellitus Canadian Journal of Diabetes 2020-11-01 [PMID: 33388277] (WB, Mouse)	WB	Mouse
Verma V, Kumar P, Gupta S et al. Alpha-Hemolysin of uropathogenic E. coli regulates NLRP3 inflammasome activation and mitochondrial dysfunction in THP-1 macrophages Sci Rep 2020-07-28 [PMID: 32724079] (WB, IP, Human)	WB, IP	Human
Cornelius DC, Travis OK, Tramel RW et al. NLRP3 inflammasome inhibition attenuates sepsis-induced platelet activation and prevents multi-organ injury in cecal-ligation puncture PLoS ONE 2020-06-17 [PMID: 32555710] (ICC/IF, Rat) Details: Citation using the Alexa Fluor 647 format of this antibody.	ICC/IF	Rat
Perez-Martinez PI, Rojas-Espinosa O, Hernandez-Chavez VG et al. Anti-inflammatory effect of omega unsaturated fatty acids and dialysable leucocyte extracts on collagen-induced arthritis in DBA/1 mice Int J Exp Pathol 2020-05-28 [PMID: 32459025] (IHC-P, Mouse)	IHC-P	Mouse
Dang S, Zhang Zy, Li Kl et Al. Blockade of beta-adrenergic signaling suppresses inflammasome and alleviates cardiac fibrosis Ann Transl Med 2020-02-01 [PMID: 32175420] (WB, Human)	WB	Human
Kariya S, Okano M, Zhao P et al. Role of Macrophage Migration Inhibitory Factor in NLRP3 Inflammasome Expression in Otitis Media Otology & Neurotology 2020-03-01 [PMID: 31821259] (IHC)	IHC
Verma V, Gupta S, Kumar P et al. Involvement of NLRP3 and NLRC4 Inflammasome in Uropathogenic E. coli Mediated Urinary Tract Infections Front Microbiol 2019-09-26 [PMID: 31551961] (IP, Human)	IP	Human
Lage SL, Dominical VM, Wong CS, Sereti I Evaluation of Canonical Inflammasome Activation in Human Monocytes by Imaging Flow Cytometry Thesis Jun 4 2019 [PMID: 31214205] (Flow, Human) Evaluation of Canonical Inflammasome Activation in Human Monocytes by Imaging Flow Cytometry. Front Immunol. 2019-06-04 [PMID: 31214205] (FLOW, Human) Details: Citation using the Alexa Fluor 647 version of this antibody.	FLOW	Human
Cornelius DC, Baik CH, Travis OK, et al. NLRP3 inflammasome activation in platelets in response to sepsis. Physiol Rep. 2019-05-03 [PMID: 31054188] (IM, Rat)	IM	Rat
Wang X, Pan J, Liu H et al. AIM2 gene silencing attenuates diabetic cardiomyopathy in type 2 diabetic rat model Life Sci. 2019-02-18 [PMID: 30790610] (WB, Rat)	WB	Rat
Kariya S, Okano M, Zhao P et al. NLRP3 inflammasome expression in lipopolysaccharide-induced otitis media. Acta Otolaryngol. 2019-01-24 [PMID: 30676848] (IHC-P, Mouse)	IHC-P	Mouse
Zhou L, Su X, Li B et al. PM2.5 exposure impairs sperm quality through testicular damage dependent on NALP3 inflammasome and miR-183/96/182 cluster targeting FOXO1 in mouse. Sci Rep. 2018-11-19 [PMID: 30476817] (WB, Mouse)	WB	Mouse
Cohen TS, Boland ML, Boland BB et al. S. aureus Evades Macrophage Killing through NLRP3-Dependent Effects on Mitochondrial Trafficking. Cell Rep. 2018-02-27 [PMID: 29490278] (Human)		Human
Liang Y, Liang S, Zhang Y et al. Oral Administration of Compound Probiotics Ameliorates HFD-Induced Gut Microbe Dysbiosis and Chronic Metabolic Inflammation via the G Protein-Coupled Receptor 43 in Non-alcoholic Fatty Liver Disease Rats. Probiotics Antimicrob Proteins 2018-01-20 [PMID: 29353414]
Zhang B, Xu D, She L et al. Silybin inhibits NLRP3 inflammasome assembly through the NAD(+)/SIRT2 pathway in mice with nonalcoholic fatty liver disease. FASEB J. 2017-09-27 [PMID: 28970254] (Mouse)		Mouse
MacPherson Maximilian, Westbom Catherine, Kogan Helen, Shukla Arti Actin polymerization plays a significant role in asbestos-induced inflammasome activation in mesothelial cells in vitro. Histochem Cell Biol. 2016-12-24 [PMID: 28013367] (ICC/IF, Human)	ICC/IF	Human
Kim SJ, Cha JY, Kang HS et al. Corosolic acid ameliorates acute inflammation through inhibition of IRAK-1 phosphorylation in macrophages. BMB Rep. 2016-05-01 [PMID: 26615974] (Mouse)		Mouse
Kariya S, Okano M, Zhao P et al. Activation of NLRP3 inflammasome in human middle ear cholesteatoma and chronic otitis media. Acta Otolaryngol 2015-10-12 [PMID: 26457439]
Yao X, Carlson D, Sun Y et al. Mitochondrial ROS Induces Cardiac Inflammation via a Pathway through mtDNA Damage in a Pneumonia-Related Sepsis Model. PLoS One 2015-01-01 [PMID: 26448624] (WB)	WB
Yao X, Wigginton JG, Maass DL et al. Estrogen-provided cardiac protection following burn trauma is mediated through a reduction in mitochondria-derived DAMPs Am J Physiol Heart Circ Physiol 2014-01-28 [PMID: 24464748] (IHC-P, Rat)	IHC-P	Rat
Show All 45 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: wang xuyang	WB	Rat	12/20/2017	View Summary Application Western Blot Sample Tested H9c2 whole cell lysate Species Rat Lot lot A-4
Enlarge	5 reviewed by: Verified Customer	WB	Mouse	05/08/2016	View Summary Application Western Blot Sample Tested Mouse macrophages Species Mouse

Array NBP1-78977

• ASC/TMS1 Antibodies
• ASC/TMS1 Lysates
• ASC/TMS1 Proteins